Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice
Grifola frondosa polysaccharide (GFP) as the natural compounds have been reported to exert diverse bioactivities. The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 diabetic mice. The changes of microbiota in faeces of the diabetic mice upon hig...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000744 |
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| author | Xiaoxiang Gao Dan Liu Luying Gao Yuezhen Ouyang Yuxi Wen Chao Ai Yuqing Chen Chao Zhao |
| author_facet | Xiaoxiang Gao Dan Liu Luying Gao Yuezhen Ouyang Yuxi Wen Chao Ai Yuqing Chen Chao Zhao |
| author_sort | Xiaoxiang Gao |
| collection | DOAJ |
| description | Grifola frondosa polysaccharide (GFP) as the natural compounds have been reported to exert diverse bioactivities. The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 diabetic mice. The changes of microbiota in faeces of the diabetic mice upon high fat diet were determined and the V3 region of the 16S rRNA was sequenced by Illumina MiSeq high-throughput sequencing platform. Eighty operational taxonomic unit were identified to be shared by all samples, and the quality and richness of sequencing were assessed via rarefaction and rank abundance curves. The diversity of gut flora was slightly improved in diabetic mice after GFP treatment. The composition and relative abundance of gut microbiota at phylum and genus levels were altered by GFP. The abundance of Robinsoniella, Flavonifractor, Anaerotruncus, and Desulfvibrio were found to concentrate on diabetic mice through LEfSe analysis. Furthermore, Alloprevotella and Burkholderia had strong relevancy with other gut flora. Based on the findings, GFP might be a desired candidate on ameliorating the intestinal unbalance in diabetes. |
| format | Article |
| id | doaj-art-2a5dbd68239f4e10a0b67c4e608541de |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-2a5dbd68239f4e10a0b67c4e608541de2025-02-03T10:41:39ZengTsinghua University PressFood Science and Human Wellness2213-45302022-01-011116873Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic miceXiaoxiang Gao0Dan Liu1Luying Gao2Yuezhen Ouyang3Yuxi Wen4Chao Ai5Yuqing Chen6Chao Zhao7College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaDepartment of Pediatrics, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Department of Food Science & Technology, National University of Singapore, Singapore 117543, SingaporeCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China; Corresponding author at: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.Grifola frondosa polysaccharide (GFP) as the natural compounds have been reported to exert diverse bioactivities. The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 diabetic mice. The changes of microbiota in faeces of the diabetic mice upon high fat diet were determined and the V3 region of the 16S rRNA was sequenced by Illumina MiSeq high-throughput sequencing platform. Eighty operational taxonomic unit were identified to be shared by all samples, and the quality and richness of sequencing were assessed via rarefaction and rank abundance curves. The diversity of gut flora was slightly improved in diabetic mice after GFP treatment. The composition and relative abundance of gut microbiota at phylum and genus levels were altered by GFP. The abundance of Robinsoniella, Flavonifractor, Anaerotruncus, and Desulfvibrio were found to concentrate on diabetic mice through LEfSe analysis. Furthermore, Alloprevotella and Burkholderia had strong relevancy with other gut flora. Based on the findings, GFP might be a desired candidate on ameliorating the intestinal unbalance in diabetes.http://www.sciencedirect.com/science/article/pii/S2213453021000744MaitakeGrifola frondosa polysaccharideDiabetesGut microbiota |
| spellingShingle | Xiaoxiang Gao Dan Liu Luying Gao Yuezhen Ouyang Yuxi Wen Chao Ai Yuqing Chen Chao Zhao Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice Food Science and Human Wellness Maitake Grifola frondosa polysaccharide Diabetes Gut microbiota |
| title | Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice |
| title_full | Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice |
| title_fullStr | Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice |
| title_full_unstemmed | Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice |
| title_short | Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice |
| title_sort | health benefits of grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice |
| topic | Maitake Grifola frondosa polysaccharide Diabetes Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000744 |
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