Bronchoalveolar Lavage Fluid IFN-γ+ Th17 Cells and Regulatory T Cells in Pulmonary Sarcoidosis
In sarcoidosis, increased Th17 cell fractions have been reported in bronchoalveolar lavage fluid, and elevated numbers of Th17 cells producing IFN-γ have been observed in peripheral blood. The balance between Th1, Th17, and FoxP3+ CD4+ T cell subsets in sarcoidosis remains unclear. Bronchoalveolar l...
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| Format: | Article |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/438070 |
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| author | Anders Tøndell Torolf Moen Magne Børset Øyvind Salvesen Anne Dorthea Rø Malcolm Sue-Chu |
| author_facet | Anders Tøndell Torolf Moen Magne Børset Øyvind Salvesen Anne Dorthea Rø Malcolm Sue-Chu |
| author_sort | Anders Tøndell |
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| description | In sarcoidosis, increased Th17 cell fractions have been reported in bronchoalveolar lavage fluid, and elevated numbers of Th17 cells producing IFN-γ have been observed in peripheral blood. The balance between Th1, Th17, and FoxP3+ CD4+ T cell subsets in sarcoidosis remains unclear. Bronchoalveolar lavage fluid cells, from 30 patients with sarcoidosis, 18 patients with other diffuse parenchymal lung diseases, and 15 healthy controls, were investigated with flow cytometry for intracellular expression of FoxP3. In a subset of the patients, expression of the cytokines IL17A and IFN-γ was investigated. The fractions of FoxP3+ CD4+ T cells and Th17 cells were both lower in sarcoidosis compared to controls (P=0.017 and P=0.011, resp.). The proportion of Th17 cells positive for IFN-γ was greater in sarcoidosis than controls (median 72.4% versus 31%, P=0.0005) and increased with radiologic stage (N=23, rho=0.45, and P=0.03). IFN-γ+ Th17 cells were highly correlated with Th1 cells (N=23, rho=0.64, and P=0.001), and the ratio of IFN-γ+ Th17/FoxP3+ CD4+ T cells was prominently increased in sarcoidosis. IFN-γ+ Th17 cells may represent a pathogenic subset of Th17 cells, yet their expression of IFN-γ could be a consequence of a Th1-polarized cytokine milieu. Our results indicate a possible immune cell imbalance in sarcoidosis. |
| format | Article |
| id | doaj-art-2a3a49141d7a436485badef405c8bf06 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2a3a49141d7a436485badef405c8bf062025-02-03T06:44:31ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/438070438070Bronchoalveolar Lavage Fluid IFN-γ+ Th17 Cells and Regulatory T Cells in Pulmonary SarcoidosisAnders Tøndell0Torolf Moen1Magne Børset2Øyvind Salvesen3Anne Dorthea Rø4Malcolm Sue-Chu5Department of Thoracic Medicine, St. Olavs University Hospital, Postboks 3250 Sluppen, 7006 Trondheim, NorwayDepartment of Immunology and Transfusion Medicine, St. Olavs University Hospital, 7006 Trondheim, NorwayDepartment of Immunology and Transfusion Medicine, St. Olavs University Hospital, 7006 Trondheim, NorwayDepartment of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, 7489 Trondheim, NorwayDepartment of Immunology and Transfusion Medicine, St. Olavs University Hospital, 7006 Trondheim, NorwayDepartment of Thoracic Medicine, St. Olavs University Hospital, Postboks 3250 Sluppen, 7006 Trondheim, NorwayIn sarcoidosis, increased Th17 cell fractions have been reported in bronchoalveolar lavage fluid, and elevated numbers of Th17 cells producing IFN-γ have been observed in peripheral blood. The balance between Th1, Th17, and FoxP3+ CD4+ T cell subsets in sarcoidosis remains unclear. Bronchoalveolar lavage fluid cells, from 30 patients with sarcoidosis, 18 patients with other diffuse parenchymal lung diseases, and 15 healthy controls, were investigated with flow cytometry for intracellular expression of FoxP3. In a subset of the patients, expression of the cytokines IL17A and IFN-γ was investigated. The fractions of FoxP3+ CD4+ T cells and Th17 cells were both lower in sarcoidosis compared to controls (P=0.017 and P=0.011, resp.). The proportion of Th17 cells positive for IFN-γ was greater in sarcoidosis than controls (median 72.4% versus 31%, P=0.0005) and increased with radiologic stage (N=23, rho=0.45, and P=0.03). IFN-γ+ Th17 cells were highly correlated with Th1 cells (N=23, rho=0.64, and P=0.001), and the ratio of IFN-γ+ Th17/FoxP3+ CD4+ T cells was prominently increased in sarcoidosis. IFN-γ+ Th17 cells may represent a pathogenic subset of Th17 cells, yet their expression of IFN-γ could be a consequence of a Th1-polarized cytokine milieu. Our results indicate a possible immune cell imbalance in sarcoidosis.http://dx.doi.org/10.1155/2014/438070 |
| spellingShingle | Anders Tøndell Torolf Moen Magne Børset Øyvind Salvesen Anne Dorthea Rø Malcolm Sue-Chu Bronchoalveolar Lavage Fluid IFN-γ+ Th17 Cells and Regulatory T Cells in Pulmonary Sarcoidosis Mediators of Inflammation |
| title | Bronchoalveolar Lavage Fluid IFN-γ+ Th17 Cells and Regulatory T Cells in Pulmonary Sarcoidosis |
| title_full | Bronchoalveolar Lavage Fluid IFN-γ+ Th17 Cells and Regulatory T Cells in Pulmonary Sarcoidosis |
| title_fullStr | Bronchoalveolar Lavage Fluid IFN-γ+ Th17 Cells and Regulatory T Cells in Pulmonary Sarcoidosis |
| title_full_unstemmed | Bronchoalveolar Lavage Fluid IFN-γ+ Th17 Cells and Regulatory T Cells in Pulmonary Sarcoidosis |
| title_short | Bronchoalveolar Lavage Fluid IFN-γ+ Th17 Cells and Regulatory T Cells in Pulmonary Sarcoidosis |
| title_sort | bronchoalveolar lavage fluid ifn γ th17 cells and regulatory t cells in pulmonary sarcoidosis |
| url | http://dx.doi.org/10.1155/2014/438070 |
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