Development of a Rapid and Sensitive Visual Pesticide Detection Card Using Crosslinked and Surface-Decorated Electrospun Nanofiber Mat
Increased consumer awareness on food safety has spurred the development of detection techniques for pesticide residues. In this study, a rapid detection card on the basis of enzyme action was developed for the visual detection of pesticides, in which the thermally crosslinked and surface-decorated p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/15/2628 |
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| author | Yunshan Wei Huange Zhou Jingxuan Kang Yongmei Wu Kun Feng |
| author_facet | Yunshan Wei Huange Zhou Jingxuan Kang Yongmei Wu Kun Feng |
| author_sort | Yunshan Wei |
| collection | DOAJ |
| description | Increased consumer awareness on food safety has spurred the development of detection techniques for pesticide residues. In this study, a rapid detection card on the basis of enzyme action was developed for the visual detection of pesticides, in which the thermally crosslinked and surface-decorated polyvinyl alcohol/citric acid nanofiber mat (PCNM) was employed as a novel immobilization matrix for acetylcholinesterase (AChE). The PCNM, crosslinked at 130 °C for 50 min, exhibited appropriate microstructure and water stability, making it suitable for AChE immobilization. The activation of carboxyl groups by surface decoration resulted in a 2.5-fold increase in enzyme loading capacity. Through parameter optimization, the detection limits for phoxim and methomyl were determined to be 0.007 mg/L and 0.10 mg/L, respectively. The detection card exhibited superior sensitivity and a reduced detection time (11 min) when compared to a commercially available pesticide detection card. Furthermore, the detection results of pesticide residues in fruit and vegetable samples confirmed its feasibility and superiority over commercial alternatives, suggesting its great potential for practical application in the on-site detection of pesticide residues. |
| format | Article |
| id | doaj-art-29d6dc96ee9d4fdc8d9b4d6b4eaa3947 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-29d6dc96ee9d4fdc8d9b4d6b4eaa39472025-08-20T04:00:53ZengMDPI AGFoods2304-81582025-07-011415262810.3390/foods14152628Development of a Rapid and Sensitive Visual Pesticide Detection Card Using Crosslinked and Surface-Decorated Electrospun Nanofiber MatYunshan Wei0Huange Zhou1Jingxuan Kang2Yongmei Wu3Kun Feng4College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, ChinaIncreased consumer awareness on food safety has spurred the development of detection techniques for pesticide residues. In this study, a rapid detection card on the basis of enzyme action was developed for the visual detection of pesticides, in which the thermally crosslinked and surface-decorated polyvinyl alcohol/citric acid nanofiber mat (PCNM) was employed as a novel immobilization matrix for acetylcholinesterase (AChE). The PCNM, crosslinked at 130 °C for 50 min, exhibited appropriate microstructure and water stability, making it suitable for AChE immobilization. The activation of carboxyl groups by surface decoration resulted in a 2.5-fold increase in enzyme loading capacity. Through parameter optimization, the detection limits for phoxim and methomyl were determined to be 0.007 mg/L and 0.10 mg/L, respectively. The detection card exhibited superior sensitivity and a reduced detection time (11 min) when compared to a commercially available pesticide detection card. Furthermore, the detection results of pesticide residues in fruit and vegetable samples confirmed its feasibility and superiority over commercial alternatives, suggesting its great potential for practical application in the on-site detection of pesticide residues.https://www.mdpi.com/2304-8158/14/15/2628nanofiber matsurface crosslinkingpesticide detectionrapid and sensitive |
| spellingShingle | Yunshan Wei Huange Zhou Jingxuan Kang Yongmei Wu Kun Feng Development of a Rapid and Sensitive Visual Pesticide Detection Card Using Crosslinked and Surface-Decorated Electrospun Nanofiber Mat Foods nanofiber mat surface crosslinking pesticide detection rapid and sensitive |
| title | Development of a Rapid and Sensitive Visual Pesticide Detection Card Using Crosslinked and Surface-Decorated Electrospun Nanofiber Mat |
| title_full | Development of a Rapid and Sensitive Visual Pesticide Detection Card Using Crosslinked and Surface-Decorated Electrospun Nanofiber Mat |
| title_fullStr | Development of a Rapid and Sensitive Visual Pesticide Detection Card Using Crosslinked and Surface-Decorated Electrospun Nanofiber Mat |
| title_full_unstemmed | Development of a Rapid and Sensitive Visual Pesticide Detection Card Using Crosslinked and Surface-Decorated Electrospun Nanofiber Mat |
| title_short | Development of a Rapid and Sensitive Visual Pesticide Detection Card Using Crosslinked and Surface-Decorated Electrospun Nanofiber Mat |
| title_sort | development of a rapid and sensitive visual pesticide detection card using crosslinked and surface decorated electrospun nanofiber mat |
| topic | nanofiber mat surface crosslinking pesticide detection rapid and sensitive |
| url | https://www.mdpi.com/2304-8158/14/15/2628 |
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