Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India
Background. Introduction of nucleic acid testing (NAT) has helped in decreasing window period donations, resulting in increased safety of blood supplies. NAT combines the advantages of direct and highly sequence-specific detection of viral genomes. We analysed the performance of newer Procleix Ultri...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2016/2543156 |
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| author | Rahul Chaurasia Diptiranjan Rout Shamsuz Zaman Kabita Chatterjee Hem Chandra Pandey Abhishek Kumar Maurya |
| author_facet | Rahul Chaurasia Diptiranjan Rout Shamsuz Zaman Kabita Chatterjee Hem Chandra Pandey Abhishek Kumar Maurya |
| author_sort | Rahul Chaurasia |
| collection | DOAJ |
| description | Background. Introduction of nucleic acid testing (NAT) has helped in decreasing window period donations, resulting in increased safety of blood supplies. NAT combines the advantages of direct and highly sequence-specific detection of viral genomes. We analysed the performance of newer Procleix Ultrio Elite (PUE) and Procleix Ultrio assay (PUA) for the screening of the viral markers in our donor population. Material and Methods. 10,015 donor samples were screened by routine immunoassays and both versions of NAT. NAT yields detected were subjected to viral load estimation and to other serological markers. Results. A total of 21 NAT yields were detected; three were positive by both NAT systems, whereas 18 samples were reactive by PUE only. NAT yields include 18 HBV and 3 HCV yields, of which 17 HBV yields were occult infections and 1 was window period (WP) infection. All 3 HCV yields were WP infections. No HIV-1/HIV-2 yield was found. Conclusion. Efficient target capture chemistry in the new TMA assay version significantly improved sensitivity. NAT is superior to serological immunoassays for screening of the viral markers; and the efficient target capture system in the newer TMA assay, namely, the PUE system, has significantly improved sensitivity over the earlier versions. |
| format | Article |
| id | doaj-art-29c4151bdb214112ac4bb4d1f8ea9af8 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-29c4151bdb214112ac4bb4d1f8ea9af82025-02-03T05:45:50ZengWileyInternational Journal of Microbiology1687-918X1687-91982016-01-01201610.1155/2016/25431562543156Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in IndiaRahul Chaurasia0Diptiranjan Rout1Shamsuz Zaman2Kabita Chatterjee3Hem Chandra Pandey4Abhishek Kumar Maurya5Department of Transfusion Medicine, All India Institute of Medical Sciences, New Delhi 110029, IndiaDepartment of Transfusion Medicine, All India Institute of Medical Sciences, New Delhi 110029, IndiaDepartment of Transfusion Medicine, All India Institute of Medical Sciences, New Delhi 110029, IndiaDepartment of Transfusion Medicine, All India Institute of Medical Sciences, New Delhi 110029, IndiaDepartment of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareilly Road, Lucknow 226014, IndiaHemogenomics Pvt. Ltd., New Delhi 110044, IndiaBackground. Introduction of nucleic acid testing (NAT) has helped in decreasing window period donations, resulting in increased safety of blood supplies. NAT combines the advantages of direct and highly sequence-specific detection of viral genomes. We analysed the performance of newer Procleix Ultrio Elite (PUE) and Procleix Ultrio assay (PUA) for the screening of the viral markers in our donor population. Material and Methods. 10,015 donor samples were screened by routine immunoassays and both versions of NAT. NAT yields detected were subjected to viral load estimation and to other serological markers. Results. A total of 21 NAT yields were detected; three were positive by both NAT systems, whereas 18 samples were reactive by PUE only. NAT yields include 18 HBV and 3 HCV yields, of which 17 HBV yields were occult infections and 1 was window period (WP) infection. All 3 HCV yields were WP infections. No HIV-1/HIV-2 yield was found. Conclusion. Efficient target capture chemistry in the new TMA assay version significantly improved sensitivity. NAT is superior to serological immunoassays for screening of the viral markers; and the efficient target capture system in the newer TMA assay, namely, the PUE system, has significantly improved sensitivity over the earlier versions.http://dx.doi.org/10.1155/2016/2543156 |
| spellingShingle | Rahul Chaurasia Diptiranjan Rout Shamsuz Zaman Kabita Chatterjee Hem Chandra Pandey Abhishek Kumar Maurya Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India International Journal of Microbiology |
| title | Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India |
| title_full | Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India |
| title_fullStr | Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India |
| title_full_unstemmed | Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India |
| title_short | Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India |
| title_sort | comparison of procleix ultrio elite and procleix ultrio nat assays for screening of transfusion transmitted infections among blood donors in india |
| url | http://dx.doi.org/10.1155/2016/2543156 |
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