Unveiling unique effector function-related bulk antibody profiles in long-term hemodialysis patients following COVID-19 mRNA booster vaccination
Background: Hemodialysis patients exhibit a reduced response to vaccination and have different vaccine dose regimens. Vaccines induce antibodies and affect the inflammatory balance through antibody glycosylation and effector functions. Therefore, we aimed to analyze the antibody glycosylation profil...
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Elsevier
2025-02-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118224001853 |
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| author | Chia-Yi Chou Chung-Yi Cheng Chih-Hsin Lee Makoto Kuro-O Tso-Hsiao Chen San-Yuan Wang Yung-Kun Chuang Yun-Jung Yang Yun-Hsuan Lin I-Lin Tsai |
| author_facet | Chia-Yi Chou Chung-Yi Cheng Chih-Hsin Lee Makoto Kuro-O Tso-Hsiao Chen San-Yuan Wang Yung-Kun Chuang Yun-Jung Yang Yun-Hsuan Lin I-Lin Tsai |
| author_sort | Chia-Yi Chou |
| collection | DOAJ |
| description | Background: Hemodialysis patients exhibit a reduced response to vaccination and have different vaccine dose regimens. Vaccines induce antibodies and affect the inflammatory balance through antibody glycosylation and effector functions. Therefore, we aimed to analyze the antibody glycosylation profiles in hemodialysis patients who were vaccinated against severe acute respiratory syndrome coronavirus 2, infected with the virus, or both, and compare them with those of dialysis patients in a control group. Methods: Plasma samples from 112 hemodialysis patients were assigned to four groups: control, infected, vaccinated, and post-vaccine-infected. Paired plasma samples from 47 people with vaccination (vaccinees) were analyzed before and after the booster dose. The same analytical approach was applied to the four groups for a cross-sectional comparison. Results: Our study found that both vaccination and infection groups showed decreased fucosylation of IgG1, which is associated with a proinflammatory biosignature. However, vaccination also leads to increased galactosylation and bisection of IgG antibodies, which are associated with anti-inflammatory effects and the additional regulation of immune responses. In contrast, infection led to an additional decrease in the fucosylation of IgG2 and IgA, demonstrating a more intense proinflammatory biosignature than vaccination. Conclusions: Our findings emphasize the proinflammatory biosignature of afucosylation in both vaccination and infection groups. Additionally, we uncovered further regulated profiles related to galactosylation in vaccinees. These findings suggest that antibody investigation for vaccination or infection should not solely focus on neutralization but should also consider effector function-related glycosylation profiling. This comprehensive information can be valuable for fine-tuning vaccine development in the future. |
| format | Article |
| id | doaj-art-29b4de47469247ffa851187871ac8775 |
| institution | Kabale University |
| issn | 1684-1182 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj-art-29b4de47469247ffa851187871ac87752025-02-06T05:11:19ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822025-02-015812737Unveiling unique effector function-related bulk antibody profiles in long-term hemodialysis patients following COVID-19 mRNA booster vaccinationChia-Yi Chou0Chung-Yi Cheng1Chih-Hsin Lee2Makoto Kuro-O3Tso-Hsiao Chen4San-Yuan Wang5Yung-Kun Chuang6Yun-Jung Yang7Yun-Hsuan Lin8I-Lin Tsai9Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, TaiwanTaipei Medical University Research Center of Urology and Kidney, Taipei, Taiwan; Division of Nephrology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Division of Nephrology, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei, TaiwanPulmonary Research Center, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan; Division of Pulmonary Medicine, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan; Division of Pulmonary Medicine, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, TaiwanDivision of Anti-Aging Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi, JapanTaipei Medical University Research Center of Urology and Kidney, Taipei, Taiwan; Division of Nephrology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Division of Nephrology, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei, TaiwanMaster Program in Clinical Genomics and Proteomics, College of Pharmacy, Taipei Medical University, Taipei, TaiwanMaster Program in Food Safety, College of Nutrition, Taipei Medical University, TaiwanGraduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, TaiwanGraduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, TaiwanGraduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taiwan; Pulmonary Research Center, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan; Master Program in Clinical Genomics and Proteomics, College of Pharmacy, Taipei Medical University, Taipei, Taiwan; Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Corresponding author. Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, No 250 Wu-Xing Street, Taipei, 110, Taiwan.Background: Hemodialysis patients exhibit a reduced response to vaccination and have different vaccine dose regimens. Vaccines induce antibodies and affect the inflammatory balance through antibody glycosylation and effector functions. Therefore, we aimed to analyze the antibody glycosylation profiles in hemodialysis patients who were vaccinated against severe acute respiratory syndrome coronavirus 2, infected with the virus, or both, and compare them with those of dialysis patients in a control group. Methods: Plasma samples from 112 hemodialysis patients were assigned to four groups: control, infected, vaccinated, and post-vaccine-infected. Paired plasma samples from 47 people with vaccination (vaccinees) were analyzed before and after the booster dose. The same analytical approach was applied to the four groups for a cross-sectional comparison. Results: Our study found that both vaccination and infection groups showed decreased fucosylation of IgG1, which is associated with a proinflammatory biosignature. However, vaccination also leads to increased galactosylation and bisection of IgG antibodies, which are associated with anti-inflammatory effects and the additional regulation of immune responses. In contrast, infection led to an additional decrease in the fucosylation of IgG2 and IgA, demonstrating a more intense proinflammatory biosignature than vaccination. Conclusions: Our findings emphasize the proinflammatory biosignature of afucosylation in both vaccination and infection groups. Additionally, we uncovered further regulated profiles related to galactosylation in vaccinees. These findings suggest that antibody investigation for vaccination or infection should not solely focus on neutralization but should also consider effector function-related glycosylation profiling. This comprehensive information can be valuable for fine-tuning vaccine development in the future.http://www.sciencedirect.com/science/article/pii/S1684118224001853ImmunoglobulinGlycosylationCOVID-19SARS-CoV-2HemodialysisVaccine |
| spellingShingle | Chia-Yi Chou Chung-Yi Cheng Chih-Hsin Lee Makoto Kuro-O Tso-Hsiao Chen San-Yuan Wang Yung-Kun Chuang Yun-Jung Yang Yun-Hsuan Lin I-Lin Tsai Unveiling unique effector function-related bulk antibody profiles in long-term hemodialysis patients following COVID-19 mRNA booster vaccination Journal of Microbiology, Immunology and Infection Immunoglobulin Glycosylation COVID-19 SARS-CoV-2 Hemodialysis Vaccine |
| title | Unveiling unique effector function-related bulk antibody profiles in long-term hemodialysis patients following COVID-19 mRNA booster vaccination |
| title_full | Unveiling unique effector function-related bulk antibody profiles in long-term hemodialysis patients following COVID-19 mRNA booster vaccination |
| title_fullStr | Unveiling unique effector function-related bulk antibody profiles in long-term hemodialysis patients following COVID-19 mRNA booster vaccination |
| title_full_unstemmed | Unveiling unique effector function-related bulk antibody profiles in long-term hemodialysis patients following COVID-19 mRNA booster vaccination |
| title_short | Unveiling unique effector function-related bulk antibody profiles in long-term hemodialysis patients following COVID-19 mRNA booster vaccination |
| title_sort | unveiling unique effector function related bulk antibody profiles in long term hemodialysis patients following covid 19 mrna booster vaccination |
| topic | Immunoglobulin Glycosylation COVID-19 SARS-CoV-2 Hemodialysis Vaccine |
| url | http://www.sciencedirect.com/science/article/pii/S1684118224001853 |
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