Differential Gene Expression Analysis in a Lumbar Spinal Stenosis Rat Model via RNA Sequencing: Identification of Key Molecular Pathways and Therapeutic Insights
Background/Objectives: Lumbar spinal stenosis (LSS) is a degenerative condition characterized by the narrowing of the spinal canal, resulting in chronic pain and impaired mobility. However, the molecular mechanisms underlying LSS remain unclear. In this study, we performed RNA sequencing (RNA-seq) t...
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2025-01-01
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| author | Jin Young Hong Wan-Jin Jeon Hyunseong Kim Changhwan Yeo Hyun Kim Yoon Jae Lee In-Hyuk Ha |
| author_facet | Jin Young Hong Wan-Jin Jeon Hyunseong Kim Changhwan Yeo Hyun Kim Yoon Jae Lee In-Hyuk Ha |
| author_sort | Jin Young Hong |
| collection | DOAJ |
| description | Background/Objectives: Lumbar spinal stenosis (LSS) is a degenerative condition characterized by the narrowing of the spinal canal, resulting in chronic pain and impaired mobility. However, the molecular mechanisms underlying LSS remain unclear. In this study, we performed RNA sequencing (RNA-seq) to investigate differential gene expression in a rat LSS model and identify the key genes and pathways involved in its pathogenesis. Methods: We used bioinformatics analysis to identify significant alterations in gene expression between the LSS-induced and sham groups. Results: Pearson’s correlation analysis demonstrated strongly consistent intragroup expression (r > 0.9), with distinct gene expression between the LSS and sham groups. A total of 113 differentially expressed genes (DEGs) were identified, including upregulated genes such as <i>Slc47a1</i> and <i>Prg4</i> and downregulated genes such as <i>Higd1c</i> and <i>Mln</i>. Functional enrichment analysis revealed that these DEGs included those involved in key biological processes, including synaptic plasticity, extracellular matrix organization, and hormonal regulation. Gene ontology analysis highlighted critical molecular functions such as mRNA binding and integrin binding, as well as cellular components such as contractile fibers and the extracellular matrix, which were significantly affected by LSS. Conclusions: Our findings provide novel insights into the molecular mechanisms underlying LSS and offer potential avenues for the development of targeted therapies aimed at mitigating disease progression and improving patient outcomes. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-299db9efd06f4a4ba5cdb852c0a105c42025-01-24T13:24:20ZengMDPI AGBiomedicines2227-90592025-01-0113119210.3390/biomedicines13010192Differential Gene Expression Analysis in a Lumbar Spinal Stenosis Rat Model via RNA Sequencing: Identification of Key Molecular Pathways and Therapeutic InsightsJin Young Hong0Wan-Jin Jeon1Hyunseong Kim2Changhwan Yeo3Hyun Kim4Yoon Jae Lee5In-Hyuk Ha6Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, Republic of KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, Republic of KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, Republic of KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, Republic of KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, Republic of KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, Republic of KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, Republic of KoreaBackground/Objectives: Lumbar spinal stenosis (LSS) is a degenerative condition characterized by the narrowing of the spinal canal, resulting in chronic pain and impaired mobility. However, the molecular mechanisms underlying LSS remain unclear. In this study, we performed RNA sequencing (RNA-seq) to investigate differential gene expression in a rat LSS model and identify the key genes and pathways involved in its pathogenesis. Methods: We used bioinformatics analysis to identify significant alterations in gene expression between the LSS-induced and sham groups. Results: Pearson’s correlation analysis demonstrated strongly consistent intragroup expression (r > 0.9), with distinct gene expression between the LSS and sham groups. A total of 113 differentially expressed genes (DEGs) were identified, including upregulated genes such as <i>Slc47a1</i> and <i>Prg4</i> and downregulated genes such as <i>Higd1c</i> and <i>Mln</i>. Functional enrichment analysis revealed that these DEGs included those involved in key biological processes, including synaptic plasticity, extracellular matrix organization, and hormonal regulation. Gene ontology analysis highlighted critical molecular functions such as mRNA binding and integrin binding, as well as cellular components such as contractile fibers and the extracellular matrix, which were significantly affected by LSS. Conclusions: Our findings provide novel insights into the molecular mechanisms underlying LSS and offer potential avenues for the development of targeted therapies aimed at mitigating disease progression and improving patient outcomes.https://www.mdpi.com/2227-9059/13/1/192lumbar spinal stenosisRNA sequencingdifferential gene expressionmolecular pathwaystherapeutic targets |
| spellingShingle | Jin Young Hong Wan-Jin Jeon Hyunseong Kim Changhwan Yeo Hyun Kim Yoon Jae Lee In-Hyuk Ha Differential Gene Expression Analysis in a Lumbar Spinal Stenosis Rat Model via RNA Sequencing: Identification of Key Molecular Pathways and Therapeutic Insights Biomedicines lumbar spinal stenosis RNA sequencing differential gene expression molecular pathways therapeutic targets |
| title | Differential Gene Expression Analysis in a Lumbar Spinal Stenosis Rat Model via RNA Sequencing: Identification of Key Molecular Pathways and Therapeutic Insights |
| title_full | Differential Gene Expression Analysis in a Lumbar Spinal Stenosis Rat Model via RNA Sequencing: Identification of Key Molecular Pathways and Therapeutic Insights |
| title_fullStr | Differential Gene Expression Analysis in a Lumbar Spinal Stenosis Rat Model via RNA Sequencing: Identification of Key Molecular Pathways and Therapeutic Insights |
| title_full_unstemmed | Differential Gene Expression Analysis in a Lumbar Spinal Stenosis Rat Model via RNA Sequencing: Identification of Key Molecular Pathways and Therapeutic Insights |
| title_short | Differential Gene Expression Analysis in a Lumbar Spinal Stenosis Rat Model via RNA Sequencing: Identification of Key Molecular Pathways and Therapeutic Insights |
| title_sort | differential gene expression analysis in a lumbar spinal stenosis rat model via rna sequencing identification of key molecular pathways and therapeutic insights |
| topic | lumbar spinal stenosis RNA sequencing differential gene expression molecular pathways therapeutic targets |
| url | https://www.mdpi.com/2227-9059/13/1/192 |
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