The Efficacy of Mesenchymal Stem Cell Transplantation in Caustic Esophagus Injury: An Experimental Study
Introduction. Ingestion of corrosive substances may lead to stricture formation in esophagus as a late complication. Full thickness injury seems to exterminate tissue stem cells of esophagus. Mesenchymal stem cells (MSCs) can differentiate into specific cell lineages and have the capacity of homing...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2014/939674 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560794564296704 |
|---|---|
| author | Murat Kantarcioglu Bahadir Caliskan Hakan Demirci Ozgur Karacalioglu Murat Kekilli Zulfikar Polat Armagan Gunal Melih Akinci Cagri Uysal Sami Eksert Hasan Gurel Gurkan Celebi Ferit Avcu Ali Ugur Ural Sait Bagci |
| author_facet | Murat Kantarcioglu Bahadir Caliskan Hakan Demirci Ozgur Karacalioglu Murat Kekilli Zulfikar Polat Armagan Gunal Melih Akinci Cagri Uysal Sami Eksert Hasan Gurel Gurkan Celebi Ferit Avcu Ali Ugur Ural Sait Bagci |
| author_sort | Murat Kantarcioglu |
| collection | DOAJ |
| description | Introduction. Ingestion of corrosive substances may lead to stricture formation in esophagus as a late complication. Full thickness injury seems to exterminate tissue stem cells of esophagus. Mesenchymal stem cells (MSCs) can differentiate into specific cell lineages and have the capacity of homing in sites of injury. Aim and Methods. We aimed to investigate the efficacy of MSC transplantation, on prevention of esophageal damage and stricture formation after caustic esophagus injury in rats. 54 rats were allocated into four groups; 4 rats were sacrificed for MSC production. Group 1, untreated controls (n: 10). Group 2, membrane labeled MSCs-treated rats (n: 20). Group 3, biodistribution of fluorodeoxyglucose labeled MSCs via positron emission tomography (PET) imaging (n: 10). Group 4, sham operated (n: 10). Standard caustic esophageal burns were created and MSCs were transplanted 24 hours after. All rats were sacrificed at the 21st days. Results. PET scan images revealed the homing behavior of MSCs to the injury site. The histopathology damage score was not significantly different from controls. However, we demonstrated Dil labeled epithelial and muscle cells which were originating from transplanted MSCs. Conclusion. MSC transplantation after caustic esophageal injury may be a helpful treatment modality; however, probably repeated infusions are needed. |
| format | Article |
| id | doaj-art-293aeb5f977e48a6ad2b0506678eebf7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-293aeb5f977e48a6ad2b0506678eebf72025-02-03T01:26:44ZengWileyStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/939674939674The Efficacy of Mesenchymal Stem Cell Transplantation in Caustic Esophagus Injury: An Experimental StudyMurat Kantarcioglu0Bahadir Caliskan1Hakan Demirci2Ozgur Karacalioglu3Murat Kekilli4Zulfikar Polat5Armagan Gunal6Melih Akinci7Cagri Uysal8Sami Eksert9Hasan Gurel10Gurkan Celebi11Ferit Avcu12Ali Ugur Ural13Sait Bagci14Department of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyDepartment of Pediatric Surgery, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyDepartment of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyDepartment of Nuclear Medicine, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyDepartment of Gastroenterology, Hitit University, 19030 Corum, TurkeyDepartment of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyDepartment of Pathology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyDepartment of General Surgery, Ankara Diskapi Training and Research Hospital, 06110 Ankara, TurkeyDepartment of Plastic and Reconstructive Surgery, Faculty of Medicine, Baskent University, 06100 Ankara, TurkeyDepartment of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyDepartment of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyDepartment of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyDepartment of Hematology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyDepartment of Hematology, Bayindir Hospital, 06250 Ankara, TurkeyDepartment of Gastroenterology, Gulhane Military Medical Academy, Etlik, 06108 Ankara, TurkeyIntroduction. Ingestion of corrosive substances may lead to stricture formation in esophagus as a late complication. Full thickness injury seems to exterminate tissue stem cells of esophagus. Mesenchymal stem cells (MSCs) can differentiate into specific cell lineages and have the capacity of homing in sites of injury. Aim and Methods. We aimed to investigate the efficacy of MSC transplantation, on prevention of esophageal damage and stricture formation after caustic esophagus injury in rats. 54 rats were allocated into four groups; 4 rats were sacrificed for MSC production. Group 1, untreated controls (n: 10). Group 2, membrane labeled MSCs-treated rats (n: 20). Group 3, biodistribution of fluorodeoxyglucose labeled MSCs via positron emission tomography (PET) imaging (n: 10). Group 4, sham operated (n: 10). Standard caustic esophageal burns were created and MSCs were transplanted 24 hours after. All rats were sacrificed at the 21st days. Results. PET scan images revealed the homing behavior of MSCs to the injury site. The histopathology damage score was not significantly different from controls. However, we demonstrated Dil labeled epithelial and muscle cells which were originating from transplanted MSCs. Conclusion. MSC transplantation after caustic esophageal injury may be a helpful treatment modality; however, probably repeated infusions are needed.http://dx.doi.org/10.1155/2014/939674 |
| spellingShingle | Murat Kantarcioglu Bahadir Caliskan Hakan Demirci Ozgur Karacalioglu Murat Kekilli Zulfikar Polat Armagan Gunal Melih Akinci Cagri Uysal Sami Eksert Hasan Gurel Gurkan Celebi Ferit Avcu Ali Ugur Ural Sait Bagci The Efficacy of Mesenchymal Stem Cell Transplantation in Caustic Esophagus Injury: An Experimental Study Stem Cells International |
| title | The Efficacy of Mesenchymal Stem Cell Transplantation in Caustic Esophagus Injury: An Experimental Study |
| title_full | The Efficacy of Mesenchymal Stem Cell Transplantation in Caustic Esophagus Injury: An Experimental Study |
| title_fullStr | The Efficacy of Mesenchymal Stem Cell Transplantation in Caustic Esophagus Injury: An Experimental Study |
| title_full_unstemmed | The Efficacy of Mesenchymal Stem Cell Transplantation in Caustic Esophagus Injury: An Experimental Study |
| title_short | The Efficacy of Mesenchymal Stem Cell Transplantation in Caustic Esophagus Injury: An Experimental Study |
| title_sort | efficacy of mesenchymal stem cell transplantation in caustic esophagus injury an experimental study |
| url | http://dx.doi.org/10.1155/2014/939674 |
| work_keys_str_mv | AT muratkantarcioglu theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT bahadircaliskan theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT hakandemirci theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT ozgurkaracalioglu theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT muratkekilli theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT zulfikarpolat theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT armagangunal theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT melihakinci theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT cagriuysal theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT samieksert theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT hasangurel theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT gurkancelebi theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT feritavcu theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT aliugurural theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT saitbagci theefficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT muratkantarcioglu efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT bahadircaliskan efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT hakandemirci efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT ozgurkaracalioglu efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT muratkekilli efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT zulfikarpolat efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT armagangunal efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT melihakinci efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT cagriuysal efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT samieksert efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT hasangurel efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT gurkancelebi efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT feritavcu efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT aliugurural efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy AT saitbagci efficacyofmesenchymalstemcelltransplantationincausticesophagusinjuryanexperimentalstudy |