Mycobacterium tuberculosis Multidrug-Resistant Strain M Induces Low IL-8 and Inhibits TNF-α Secretion by Bronchial Epithelial Cells Altering Neutrophil Effector Functions
M strain, the most prevalent multidrug-resistant strain of Mycobacterium tuberculosis (Mtb) in Argentina, has mounted mechanisms to evade innate immune response. The role of human bronchial epithelium in Mtb infection remains unknown as well as its crosstalk with neutrophils (PMN). In this work, we...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/2810606 |
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| author | Denise Kviatcovsky Leonardo Rivadeneyra Luciana Balboa Noemí Yokobori Beatriz López Viviana Ritacco Mirta Schattner María del Carmen Sasiain Silvia de la Barrera |
| author_facet | Denise Kviatcovsky Leonardo Rivadeneyra Luciana Balboa Noemí Yokobori Beatriz López Viviana Ritacco Mirta Schattner María del Carmen Sasiain Silvia de la Barrera |
| author_sort | Denise Kviatcovsky |
| collection | DOAJ |
| description | M strain, the most prevalent multidrug-resistant strain of Mycobacterium tuberculosis (Mtb) in Argentina, has mounted mechanisms to evade innate immune response. The role of human bronchial epithelium in Mtb infection remains unknown as well as its crosstalk with neutrophils (PMN). In this work, we evaluate whether M and H37Rv strains invade and replicate within bronchial epithelial cell line Calu-6 and how conditioned media (CM) derived from infected cells alter PMN responses. We demonstrated that M infects and survives within Calu-6 without promoting death. CM from M-infected Calu-6 (M-CM) did not attract PMN in correlation with its low IL-8 content compared to H37Rv-CM. Also, PMN activation and ROS production in response to irradiated H37Rv were impaired after treatment with M-CM due to the lack of TNF-α. Interestingly, M-CM increased H37Rv replication in PMN which would allow the spreading of mycobacteria upon PMN death and sustain IL-8 release. Thus, our results indicate that even at low invasion/replication rate within Calu-6, M induces the secretion of factors altering the crosstalk between these nonphagocytic cells and PMN, representing an evasion mechanism developed by M strain to persist in the host. These data provide new insights on the role of bronchial epithelium upon M infection. |
| format | Article |
| id | doaj-art-2904367d72b84a20acbf6aa2afcc25ce |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2904367d72b84a20acbf6aa2afcc25ce2025-02-03T06:42:13ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/28106062810606Mycobacterium tuberculosis Multidrug-Resistant Strain M Induces Low IL-8 and Inhibits TNF-α Secretion by Bronchial Epithelial Cells Altering Neutrophil Effector FunctionsDenise Kviatcovsky0Leonardo Rivadeneyra1Luciana Balboa2Noemí Yokobori3Beatriz López4Viviana Ritacco5Mirta Schattner6María del Carmen Sasiain7Silvia de la Barrera8Instituto de Medicina Experimental-CONICET-Academia Nacional de Medicina, Buenos Aires, ArgentinaInstituto de Medicina Experimental-CONICET-Academia Nacional de Medicina, Buenos Aires, ArgentinaInstituto de Medicina Experimental-CONICET-Academia Nacional de Medicina, Buenos Aires, ArgentinaInstituto de Medicina Experimental-CONICET-Academia Nacional de Medicina, Buenos Aires, ArgentinaInstituto Nacional de Enfermedades Infecciosas, ANLIS Carlos G. Malbrán, Buenos Aires, ArgentinaInstituto Nacional de Enfermedades Infecciosas, ANLIS Carlos G. Malbrán, Buenos Aires, ArgentinaInstituto de Medicina Experimental-CONICET-Academia Nacional de Medicina, Buenos Aires, ArgentinaInstituto de Medicina Experimental-CONICET-Academia Nacional de Medicina, Buenos Aires, ArgentinaInstituto de Medicina Experimental-CONICET-Academia Nacional de Medicina, Buenos Aires, ArgentinaM strain, the most prevalent multidrug-resistant strain of Mycobacterium tuberculosis (Mtb) in Argentina, has mounted mechanisms to evade innate immune response. The role of human bronchial epithelium in Mtb infection remains unknown as well as its crosstalk with neutrophils (PMN). In this work, we evaluate whether M and H37Rv strains invade and replicate within bronchial epithelial cell line Calu-6 and how conditioned media (CM) derived from infected cells alter PMN responses. We demonstrated that M infects and survives within Calu-6 without promoting death. CM from M-infected Calu-6 (M-CM) did not attract PMN in correlation with its low IL-8 content compared to H37Rv-CM. Also, PMN activation and ROS production in response to irradiated H37Rv were impaired after treatment with M-CM due to the lack of TNF-α. Interestingly, M-CM increased H37Rv replication in PMN which would allow the spreading of mycobacteria upon PMN death and sustain IL-8 release. Thus, our results indicate that even at low invasion/replication rate within Calu-6, M induces the secretion of factors altering the crosstalk between these nonphagocytic cells and PMN, representing an evasion mechanism developed by M strain to persist in the host. These data provide new insights on the role of bronchial epithelium upon M infection.http://dx.doi.org/10.1155/2017/2810606 |
| spellingShingle | Denise Kviatcovsky Leonardo Rivadeneyra Luciana Balboa Noemí Yokobori Beatriz López Viviana Ritacco Mirta Schattner María del Carmen Sasiain Silvia de la Barrera Mycobacterium tuberculosis Multidrug-Resistant Strain M Induces Low IL-8 and Inhibits TNF-α Secretion by Bronchial Epithelial Cells Altering Neutrophil Effector Functions Mediators of Inflammation |
| title | Mycobacterium tuberculosis Multidrug-Resistant Strain M Induces Low IL-8 and Inhibits TNF-α Secretion by Bronchial Epithelial Cells Altering Neutrophil Effector Functions |
| title_full | Mycobacterium tuberculosis Multidrug-Resistant Strain M Induces Low IL-8 and Inhibits TNF-α Secretion by Bronchial Epithelial Cells Altering Neutrophil Effector Functions |
| title_fullStr | Mycobacterium tuberculosis Multidrug-Resistant Strain M Induces Low IL-8 and Inhibits TNF-α Secretion by Bronchial Epithelial Cells Altering Neutrophil Effector Functions |
| title_full_unstemmed | Mycobacterium tuberculosis Multidrug-Resistant Strain M Induces Low IL-8 and Inhibits TNF-α Secretion by Bronchial Epithelial Cells Altering Neutrophil Effector Functions |
| title_short | Mycobacterium tuberculosis Multidrug-Resistant Strain M Induces Low IL-8 and Inhibits TNF-α Secretion by Bronchial Epithelial Cells Altering Neutrophil Effector Functions |
| title_sort | mycobacterium tuberculosis multidrug resistant strain m induces low il 8 and inhibits tnf α secretion by bronchial epithelial cells altering neutrophil effector functions |
| url | http://dx.doi.org/10.1155/2017/2810606 |
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