Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma
Background. Thrombospondin type 1 domain-containing 7A (THSD7A) was reported to play a procancer role in esophageal squamous cell carcinoma (ESCC). The aim of the study was to screen the downstream functional genes of THSD7A and explore their functions in ESCC, based on the reported research into TH...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/2555647 |
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| author | Kawuli Jumai Tangjuan Zhang Bingzhang Qiao Julaiti Ainiwaer Haiping Zhang Zhichao Hou Idris Awut Madinyat Niyaz Liwei Zhang Ilyar Sheyhidin |
| author_facet | Kawuli Jumai Tangjuan Zhang Bingzhang Qiao Julaiti Ainiwaer Haiping Zhang Zhichao Hou Idris Awut Madinyat Niyaz Liwei Zhang Ilyar Sheyhidin |
| author_sort | Kawuli Jumai |
| collection | DOAJ |
| description | Background. Thrombospondin type 1 domain-containing 7A (THSD7A) was reported to play a procancer role in esophageal squamous cell carcinoma (ESCC). The aim of the study was to screen the downstream functional genes of THSD7A and explore their functions in ESCC, based on the reported research into THSD7A function and on gene microarrays. Methods. We adopted quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Celigo high-content screening (HCS) technology to screen the downstream genes of THSD7A. The expression level of target genes was examined by PCR, western blot, and immunohistochemistry (IHC). The effects of these target genes on ESCC malignant biological behavior were performed in vivo and in vitro. The Kaplan-Meier (K-M) survival analysis and Cox regression were used to analyze the prognostic significance of target genes in ESCC patients. Experiments in the literature on liver cancer (LC) were repeated to verify the functions of these genes in different tumors. We further explored the cancer-promoting mechanism of target genes in ESCC by sequencing of the genes’ exons. Results. Scavenger receptor class A member 5 (SCARA5) was proved to be the downstream driving gene of THSD7A. SCARA5 promoted cell proliferation and migration but inhibited apoptosis in ESCC. IHC results confirmed that SCARA5 expression in ESCC exceeded that in normal tissues. The K-M survival analysis indicated that SCARA5 expression quantity was not related to prognosis, but tumor volume and T classification were both the independent prognostic factors. Repetition of experiments in LC in the literature confirmed that SCARA5 had exactly opposite functions in EC and LC. Conclusion. SCARA5 was related to the development and occurrence of ESCC. Our findings suggested that it was a potentially diagnostic individualized therapeutic target for ESCC in the future and that its application could possibly be combined with that of upstream THSD7A gene. |
| format | Article |
| id | doaj-art-289cb040fb6a48898bd96cade1480168 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-289cb040fb6a48898bd96cade14801682025-02-03T05:50:31ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/2555647Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell CarcinomaKawuli Jumai0Tangjuan Zhang1Bingzhang Qiao2Julaiti Ainiwaer3Haiping Zhang4Zhichao Hou5Idris Awut6Madinyat Niyaz7Liwei Zhang8Ilyar Sheyhidin9Department of Thoracic SurgeryDepartment of EmergencyDepartment of Thoracic SurgeryDepartment of Thoracic SurgeryDepartment of Thoracic SurgeryDepartment of Thoracic SurgeryDepartment of Thoracic SurgeryState Key Laboratory of PathogenesisDepartment of Thoracic SurgeryDepartment of Thoracic SurgeryBackground. Thrombospondin type 1 domain-containing 7A (THSD7A) was reported to play a procancer role in esophageal squamous cell carcinoma (ESCC). The aim of the study was to screen the downstream functional genes of THSD7A and explore their functions in ESCC, based on the reported research into THSD7A function and on gene microarrays. Methods. We adopted quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Celigo high-content screening (HCS) technology to screen the downstream genes of THSD7A. The expression level of target genes was examined by PCR, western blot, and immunohistochemistry (IHC). The effects of these target genes on ESCC malignant biological behavior were performed in vivo and in vitro. The Kaplan-Meier (K-M) survival analysis and Cox regression were used to analyze the prognostic significance of target genes in ESCC patients. Experiments in the literature on liver cancer (LC) were repeated to verify the functions of these genes in different tumors. We further explored the cancer-promoting mechanism of target genes in ESCC by sequencing of the genes’ exons. Results. Scavenger receptor class A member 5 (SCARA5) was proved to be the downstream driving gene of THSD7A. SCARA5 promoted cell proliferation and migration but inhibited apoptosis in ESCC. IHC results confirmed that SCARA5 expression in ESCC exceeded that in normal tissues. The K-M survival analysis indicated that SCARA5 expression quantity was not related to prognosis, but tumor volume and T classification were both the independent prognostic factors. Repetition of experiments in LC in the literature confirmed that SCARA5 had exactly opposite functions in EC and LC. Conclusion. SCARA5 was related to the development and occurrence of ESCC. Our findings suggested that it was a potentially diagnostic individualized therapeutic target for ESCC in the future and that its application could possibly be combined with that of upstream THSD7A gene.http://dx.doi.org/10.1155/2022/2555647 |
| spellingShingle | Kawuli Jumai Tangjuan Zhang Bingzhang Qiao Julaiti Ainiwaer Haiping Zhang Zhichao Hou Idris Awut Madinyat Niyaz Liwei Zhang Ilyar Sheyhidin Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma Journal of Immunology Research |
| title | Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma |
| title_full | Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma |
| title_fullStr | Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma |
| title_full_unstemmed | Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma |
| title_short | Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma |
| title_sort | highly expressing scara5 promotes proliferation and migration of esophageal squamous cell carcinoma |
| url | http://dx.doi.org/10.1155/2022/2555647 |
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