Radioiodine Labeled Anti-MIF McAb: A Potential Agent for Inflammation Imaging
Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that may play a role in the pathogenesis of inflammation. Radiolabeled anti-MIF McAb can be used to detect in vivo inflammatory changes. The objective of this study was to investigat...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2007/50180 |
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| author | Chao Zhang Gui-hua Hou Jian-kui Han Jing Song Ting Liang |
| author_facet | Chao Zhang Gui-hua Hou Jian-kui Han Jing Song Ting Liang |
| author_sort | Chao Zhang |
| collection | DOAJ |
| description | Macrophage migration inhibitory factor (MIF) is
a proinflammatory cytokine that may play a role in the
pathogenesis of inflammation. Radiolabeled anti-MIF
McAb can be used
to detect in vivo inflammatory changes. The objective of this study was to investigate in vivo
biology of radioiodinated anti-MIF McAb using the inflammation model mice. Anti-MIF McAb
was radioiodinated with NaI125
by Iodogen method. Animal models were induced in the mice
by intramuscular injection of S. aureus, E. coli, and
turpentine oil. The biodistribution studies
with radioiodinated anti-MIF McAb were performed on inflammation mice. The relationship
between inflammatory lesions and anti-MIF McAb binding was investigated using the percent
of injected dose per gram tissue (% ID/g) of tissue samples and whole-body autoradiography.
The radioactivity of I125-anti-MIF McAb in the inflammatory tissue increased gradually for three
inflammation models. The highest uptake was found in S. aureus group
and the lowest was in E. coli group. The uptake in turpentine oil group was
average. Whole-body autoradiography showed that all inflammation foci could be visualized
clearly from 24 hours after injection, but 48 hours images were much clearer in accordance
with the high T/NT ratio. These results demonstrate the ability of radioiodinated anti-MIF McAb
to measure in vivo inflammatory events represented by high expression of MIF and suggests
that radiolabeled anti-MIF McAb warrants further investigation as a potential inflammation-seeking
agent for imaging to detect inflammatory disorders. |
| format | Article |
| id | doaj-art-2884fe1388da404c939ea6f39ca5ebf5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2884fe1388da404c939ea6f39ca5ebf52025-02-03T05:51:42ZengWileyMediators of Inflammation0962-93511466-18612007-01-01200710.1155/2007/5018050180Radioiodine Labeled Anti-MIF McAb: A Potential Agent for Inflammation ImagingChao Zhang0Gui-hua Hou1Jian-kui Han2Jing Song3Ting Liang4Department of Nuclear Medicine, Qilu Hospital of Shandong University, Jinan 250012, ChinaInstitute of Experimental Nuclear Medicine, School of Medicine, Shandong University, Jinan 250012, ChinaDepartment of Nuclear Medicine, Qilu Hospital of Shandong University, Jinan 250012, ChinaInstitute of Experimental Nuclear Medicine, School of Medicine, Shandong University, Jinan 250012, ChinaInstitute of Experimental Nuclear Medicine, School of Medicine, Shandong University, Jinan 250012, ChinaMacrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that may play a role in the pathogenesis of inflammation. Radiolabeled anti-MIF McAb can be used to detect in vivo inflammatory changes. The objective of this study was to investigate in vivo biology of radioiodinated anti-MIF McAb using the inflammation model mice. Anti-MIF McAb was radioiodinated with NaI125 by Iodogen method. Animal models were induced in the mice by intramuscular injection of S. aureus, E. coli, and turpentine oil. The biodistribution studies with radioiodinated anti-MIF McAb were performed on inflammation mice. The relationship between inflammatory lesions and anti-MIF McAb binding was investigated using the percent of injected dose per gram tissue (% ID/g) of tissue samples and whole-body autoradiography. The radioactivity of I125-anti-MIF McAb in the inflammatory tissue increased gradually for three inflammation models. The highest uptake was found in S. aureus group and the lowest was in E. coli group. The uptake in turpentine oil group was average. Whole-body autoradiography showed that all inflammation foci could be visualized clearly from 24 hours after injection, but 48 hours images were much clearer in accordance with the high T/NT ratio. These results demonstrate the ability of radioiodinated anti-MIF McAb to measure in vivo inflammatory events represented by high expression of MIF and suggests that radiolabeled anti-MIF McAb warrants further investigation as a potential inflammation-seeking agent for imaging to detect inflammatory disorders.http://dx.doi.org/10.1155/2007/50180 |
| spellingShingle | Chao Zhang Gui-hua Hou Jian-kui Han Jing Song Ting Liang Radioiodine Labeled Anti-MIF McAb: A Potential Agent for Inflammation Imaging Mediators of Inflammation |
| title | Radioiodine Labeled Anti-MIF McAb: A Potential Agent for Inflammation Imaging |
| title_full | Radioiodine Labeled Anti-MIF McAb: A Potential Agent for Inflammation Imaging |
| title_fullStr | Radioiodine Labeled Anti-MIF McAb: A Potential Agent for Inflammation Imaging |
| title_full_unstemmed | Radioiodine Labeled Anti-MIF McAb: A Potential Agent for Inflammation Imaging |
| title_short | Radioiodine Labeled Anti-MIF McAb: A Potential Agent for Inflammation Imaging |
| title_sort | radioiodine labeled anti mif mcab a potential agent for inflammation imaging |
| url | http://dx.doi.org/10.1155/2007/50180 |
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