Radioiodine Labeled Anti-MIF McAb: A Potential Agent for Inflammation Imaging
Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that may play a role in the pathogenesis of inflammation. Radiolabeled anti-MIF McAb can be used to detect in vivo inflammatory changes. The objective of this study was to investigat...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2007-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2007/50180 |
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| Summary: | Macrophage migration inhibitory factor (MIF) is
a proinflammatory cytokine that may play a role in the
pathogenesis of inflammation. Radiolabeled anti-MIF
McAb can be used
to detect in vivo inflammatory changes. The objective of this study was to investigate in vivo
biology of radioiodinated anti-MIF McAb using the inflammation model mice. Anti-MIF McAb
was radioiodinated with NaI125
by Iodogen method. Animal models were induced in the mice
by intramuscular injection of S. aureus, E. coli, and
turpentine oil. The biodistribution studies
with radioiodinated anti-MIF McAb were performed on inflammation mice. The relationship
between inflammatory lesions and anti-MIF McAb binding was investigated using the percent
of injected dose per gram tissue (% ID/g) of tissue samples and whole-body autoradiography.
The radioactivity of I125-anti-MIF McAb in the inflammatory tissue increased gradually for three
inflammation models. The highest uptake was found in S. aureus group
and the lowest was in E. coli group. The uptake in turpentine oil group was
average. Whole-body autoradiography showed that all inflammation foci could be visualized
clearly from 24 hours after injection, but 48 hours images were much clearer in accordance
with the high T/NT ratio. These results demonstrate the ability of radioiodinated anti-MIF McAb
to measure in vivo inflammatory events represented by high expression of MIF and suggests
that radiolabeled anti-MIF McAb warrants further investigation as a potential inflammation-seeking
agent for imaging to detect inflammatory disorders. |
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| ISSN: | 0962-9351 1466-1861 |