Changes in Digestive Enzyme Activities during Larval Development of Spotted Seatrout (Cynoscion nebulosus)
The spotted seatrout (Cynoscion nebulosus)—an important commercial species—has a high potential for aquaculture in the Gulf of Mexico. To optimize its feeding during larval rearing, this study aims to evaluate the primary gastric (pepsin), intestinal (leucine aminopeptidase and alkaline phosphatase)...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/2024/1309390 |
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| author | Martín Alberto Arenas-Pardo Martha Gabriela Gaxiola-Cortés Alvaro Fabricio Barreto-Altamirano Adriana del Carmen Paredes-Medina Iveth Gabriela Palomino-Albarrán Patricia Margarita Balam-Uc Juan Carlos Maldonado-Flores Carlos Alfonso Álvarez-González |
| author_facet | Martín Alberto Arenas-Pardo Martha Gabriela Gaxiola-Cortés Alvaro Fabricio Barreto-Altamirano Adriana del Carmen Paredes-Medina Iveth Gabriela Palomino-Albarrán Patricia Margarita Balam-Uc Juan Carlos Maldonado-Flores Carlos Alfonso Álvarez-González |
| author_sort | Martín Alberto Arenas-Pardo |
| collection | DOAJ |
| description | The spotted seatrout (Cynoscion nebulosus)—an important commercial species—has a high potential for aquaculture in the Gulf of Mexico. To optimize its feeding during larval rearing, this study aims to evaluate the primary gastric (pepsin), intestinal (leucine aminopeptidase and alkaline phosphatase), and pancreatic (alkaline protease, trypsin, chymotrypsin, amylase, and lipase) enzyme activities from hatching to day 30. A multivariate analysis identified three digestive enzyme development stages during the spotted seatrout larval transformation. The first stage occurred between 1 (mean ± standard error (SE) = 1.73 ± 0.14 millimeter (mm) standard length (SL)) and 3 (2.14 ± 0.07 mm SL) days after hatching (DAH); a period of digestive stability showed the highest activity in amylase and bile salt-dependent lipase. The second stage (from 4 (2.53 ± 0.09 mm SL) to 20 (10.92 ± 0.51 mm SL) DAH) was a period of digestive transition, during which leucine aminopeptidase, chymotrypsin, and alkaline proteases were identified as the predominant enzymes from 4 to 5 DAH. In the third stage—a period of digestive stability—pepsin was the major enzyme that occurred between 25 (16.51 ± 0.81 mm SL) and 30 (25.91 ± 0.82 mm SL) DAH. These results indicate that the spotted seatrout larvae have a digestive system adapted to lipids and carbohydrates at the onset of feeding, with an immediate transition to protein digestion when exogenous feeding begins. Additionally, the digestive system of the spotted seatrout may be considered mature at 25 DAH. Further research is needed to elucidate the mechanisms of digestive tract development in the spotted seatrout larvae. |
| format | Article |
| id | doaj-art-2859289c455b4690aaf910f57ab4b537 |
| institution | Kabale University |
| issn | 1365-2095 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture Nutrition |
| spelling | doaj-art-2859289c455b4690aaf910f57ab4b5372025-02-03T05:54:44ZengWileyAquaculture Nutrition1365-20952024-01-01202410.1155/2024/1309390Changes in Digestive Enzyme Activities during Larval Development of Spotted Seatrout (Cynoscion nebulosus)Martín Alberto Arenas-Pardo0Martha Gabriela Gaxiola-Cortés1Alvaro Fabricio Barreto-Altamirano2Adriana del Carmen Paredes-Medina3Iveth Gabriela Palomino-Albarrán4Patricia Margarita Balam-Uc5Juan Carlos Maldonado-Flores6Carlos Alfonso Álvarez-González7Laboratorio de Fisiología en Recursos AcuáticosUnidad Multidisciplinaria de Docencia e Investigación (UMDI) SisalUnidad Multidisciplinaria de Docencia e Investigación (UMDI) SisalUnidad Multidisciplinaria de Docencia e Investigación (UMDI) SisalUnidad Multidisciplinaria de Docencia e Investigación (UMDI) SisalUnidad Multidisciplinaria de Docencia e Investigación (UMDI) SisalUnidad Multidisciplinaria de Docencia e Investigación (UMDI) SisalLaboratorio de Fisiología en Recursos AcuáticosThe spotted seatrout (Cynoscion nebulosus)—an important commercial species—has a high potential for aquaculture in the Gulf of Mexico. To optimize its feeding during larval rearing, this study aims to evaluate the primary gastric (pepsin), intestinal (leucine aminopeptidase and alkaline phosphatase), and pancreatic (alkaline protease, trypsin, chymotrypsin, amylase, and lipase) enzyme activities from hatching to day 30. A multivariate analysis identified three digestive enzyme development stages during the spotted seatrout larval transformation. The first stage occurred between 1 (mean ± standard error (SE) = 1.73 ± 0.14 millimeter (mm) standard length (SL)) and 3 (2.14 ± 0.07 mm SL) days after hatching (DAH); a period of digestive stability showed the highest activity in amylase and bile salt-dependent lipase. The second stage (from 4 (2.53 ± 0.09 mm SL) to 20 (10.92 ± 0.51 mm SL) DAH) was a period of digestive transition, during which leucine aminopeptidase, chymotrypsin, and alkaline proteases were identified as the predominant enzymes from 4 to 5 DAH. In the third stage—a period of digestive stability—pepsin was the major enzyme that occurred between 25 (16.51 ± 0.81 mm SL) and 30 (25.91 ± 0.82 mm SL) DAH. These results indicate that the spotted seatrout larvae have a digestive system adapted to lipids and carbohydrates at the onset of feeding, with an immediate transition to protein digestion when exogenous feeding begins. Additionally, the digestive system of the spotted seatrout may be considered mature at 25 DAH. Further research is needed to elucidate the mechanisms of digestive tract development in the spotted seatrout larvae.http://dx.doi.org/10.1155/2024/1309390 |
| spellingShingle | Martín Alberto Arenas-Pardo Martha Gabriela Gaxiola-Cortés Alvaro Fabricio Barreto-Altamirano Adriana del Carmen Paredes-Medina Iveth Gabriela Palomino-Albarrán Patricia Margarita Balam-Uc Juan Carlos Maldonado-Flores Carlos Alfonso Álvarez-González Changes in Digestive Enzyme Activities during Larval Development of Spotted Seatrout (Cynoscion nebulosus) Aquaculture Nutrition |
| title | Changes in Digestive Enzyme Activities during Larval Development of Spotted Seatrout (Cynoscion nebulosus) |
| title_full | Changes in Digestive Enzyme Activities during Larval Development of Spotted Seatrout (Cynoscion nebulosus) |
| title_fullStr | Changes in Digestive Enzyme Activities during Larval Development of Spotted Seatrout (Cynoscion nebulosus) |
| title_full_unstemmed | Changes in Digestive Enzyme Activities during Larval Development of Spotted Seatrout (Cynoscion nebulosus) |
| title_short | Changes in Digestive Enzyme Activities during Larval Development of Spotted Seatrout (Cynoscion nebulosus) |
| title_sort | changes in digestive enzyme activities during larval development of spotted seatrout cynoscion nebulosus |
| url | http://dx.doi.org/10.1155/2024/1309390 |
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