Defense responses of the green microalgae Chlorella vulgaris to the vanadium pentoxide nanoparticles
Although vanadium-based nanomaterials have found extensive use in industry, their influence on ecosystems and living organisms is not yet well investigated. In this study, hydrothermal methods were utilized for the synthesis of vanadium pentoxide nanoparticles (V2O5 NPs). The gained NPs were charact...
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| Format: | Article |
| Language: | English |
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Sciendo
2023-12-01
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| Series: | Oceanological and Hydrobiological Studies |
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| Online Access: | https://doi.org/10.26881/oahs-2023.4.06 |
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| author | Kalehjahi Hanifeh Akbarian Kosari-Nasab Morteza Amini Mojtaba Movafeghi Ali |
| author_facet | Kalehjahi Hanifeh Akbarian Kosari-Nasab Morteza Amini Mojtaba Movafeghi Ali |
| author_sort | Kalehjahi Hanifeh Akbarian |
| collection | DOAJ |
| description | Although vanadium-based nanomaterials have found extensive use in industry, their influence on ecosystems and living organisms is not yet well investigated. In this study, hydrothermal methods were utilized for the synthesis of vanadium pentoxide nanoparticles (V2O5 NPs). The gained NPs were characterized using XRD, FT-IR, EDS, DLS, SEM and TEM techniques. Subsequently, the toxic effects of V2O5 NPs on the model green microalgae Chlorella vulgaris were evaluated. According to the obtained results, V2O5 NPs caused a significant reduction in cell number and biomass production of algae in a dose and time dependent manner. Moreover, flow cytometric analysis confirmed a reduction in the quantity of living cells. Scanning electron microscopy showed plasmolysis and deformation of the cells after exposure to nanoparticles. The photosynthetic pigments and phenolics content exhibited a decrease in comparison with the control sample. Although, non-enzymatic antioxidant system in C. vulgaris displayed an average action, antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) showed a dose dependent increasing trend. These intercellular reactions designated the activation of the antioxidant defense system in response to the induced oxidative stress by V2O5 NPs. |
| format | Article |
| id | doaj-art-28134ac2cb6b47908131670c9d3f1851 |
| institution | Kabale University |
| issn | 1897-3191 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Oceanological and Hydrobiological Studies |
| spelling | doaj-art-28134ac2cb6b47908131670c9d3f18512025-01-20T11:10:23ZengSciendoOceanological and Hydrobiological Studies1897-31912023-12-0152444646010.26881/oahs-2023.4.06Defense responses of the green microalgae Chlorella vulgaris to the vanadium pentoxide nanoparticlesKalehjahi Hanifeh Akbarian0Kosari-Nasab Morteza1Amini Mojtaba2Movafeghi Ali3Department of Plant, Cell and Molecular Biology, University of Tabriz, Tabriz, Azarbayjane Sharghi, Islamic Republic of IranDepartment of Plant, Cell and Molecular Biology, University of Tabriz, Tabriz, Azarbayjane Sharghi, Islamic Republic of IranDepartment of Inorganic Chemistry, University of Tabriz, Tabriz, Azarbayjane Sharghi, Islamic Republic of IranDepartment of Plant, Cell and Molecular Biology, University of Tabriz, Tabriz, Azarbayjane Sharghi, Islamic Republic of IranAlthough vanadium-based nanomaterials have found extensive use in industry, their influence on ecosystems and living organisms is not yet well investigated. In this study, hydrothermal methods were utilized for the synthesis of vanadium pentoxide nanoparticles (V2O5 NPs). The gained NPs were characterized using XRD, FT-IR, EDS, DLS, SEM and TEM techniques. Subsequently, the toxic effects of V2O5 NPs on the model green microalgae Chlorella vulgaris were evaluated. According to the obtained results, V2O5 NPs caused a significant reduction in cell number and biomass production of algae in a dose and time dependent manner. Moreover, flow cytometric analysis confirmed a reduction in the quantity of living cells. Scanning electron microscopy showed plasmolysis and deformation of the cells after exposure to nanoparticles. The photosynthetic pigments and phenolics content exhibited a decrease in comparison with the control sample. Although, non-enzymatic antioxidant system in C. vulgaris displayed an average action, antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) showed a dose dependent increasing trend. These intercellular reactions designated the activation of the antioxidant defense system in response to the induced oxidative stress by V2O5 NPs.https://doi.org/10.26881/oahs-2023.4.06chlorella vulgarisalgaev2o5 nanoparticlesoxidative stressantioxidant enzymes |
| spellingShingle | Kalehjahi Hanifeh Akbarian Kosari-Nasab Morteza Amini Mojtaba Movafeghi Ali Defense responses of the green microalgae Chlorella vulgaris to the vanadium pentoxide nanoparticles Oceanological and Hydrobiological Studies chlorella vulgaris algae v2o5 nanoparticles oxidative stress antioxidant enzymes |
| title | Defense responses of the green microalgae Chlorella vulgaris to the vanadium pentoxide nanoparticles |
| title_full | Defense responses of the green microalgae Chlorella vulgaris to the vanadium pentoxide nanoparticles |
| title_fullStr | Defense responses of the green microalgae Chlorella vulgaris to the vanadium pentoxide nanoparticles |
| title_full_unstemmed | Defense responses of the green microalgae Chlorella vulgaris to the vanadium pentoxide nanoparticles |
| title_short | Defense responses of the green microalgae Chlorella vulgaris to the vanadium pentoxide nanoparticles |
| title_sort | defense responses of the green microalgae chlorella vulgaris to the vanadium pentoxide nanoparticles |
| topic | chlorella vulgaris algae v2o5 nanoparticles oxidative stress antioxidant enzymes |
| url | https://doi.org/10.26881/oahs-2023.4.06 |
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