Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya
In this study, the possibility of sexual reproduction in sampled Aspergillus flavus strains was evaluated by assessing the distribution of mating type (MAT) genes, which are known to control sexual character among fungi, for two counties in Kenya. Forty-four isolates from Nandi and Makueni counties...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Advances in Agriculture |
| Online Access: | http://dx.doi.org/10.1155/2018/3095096 |
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| author | Ouko Abigael Okoth Sheila Amugune Nelson Vesa Joutsjoki |
| author_facet | Ouko Abigael Okoth Sheila Amugune Nelson Vesa Joutsjoki |
| author_sort | Ouko Abigael |
| collection | DOAJ |
| description | In this study, the possibility of sexual reproduction in sampled Aspergillus flavus strains was evaluated by assessing the distribution of mating type (MAT) genes, which are known to control sexual character among fungi, for two counties in Kenya. Forty-four isolates from Nandi and Makueni counties were genotyped by MAT using a multiplex polymerase chain reaction assay. The primer pair for the MAT1-1 amplified a 396 base pair (bp) fragment containing an α-box motif, and MAT1-2 primers targeted a 270 bp segment with a high mobility group protein. The MAT1-2 genes dominated in both regions although the frequency was higher in Nandi (75%) than in Makueni (54.17%). There were no MAT1-1 genes sampled in Nandi, and in Makueni their proportion was 15.91%. The percentage of isolates that amplified for both MAT genes in Makueni was 9.09%, while in Nandi it was 11.36%. Currently, use of aggressive aflatoxin non-producing A. flavus strains as biocontrol is the most promising preharvest aflatoxin control strategy in Kenya. However, we address the possibility of introduced biocontrol strains to breed with existing aflatoxin producing strains in nature, which could lead to the generation of A. flavus offspring capable of aflatoxin production while also being aggressive colonizers and possibly increasing the burden of aflatoxin exposure in food. |
| format | Article |
| id | doaj-art-280e51b397db44f3981a8c786a687f40 |
| institution | Kabale University |
| issn | 2356-654X 2314-7539 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Agriculture |
| spelling | doaj-art-280e51b397db44f3981a8c786a687f402025-02-03T01:30:13ZengWileyAdvances in Agriculture2356-654X2314-75392018-01-01201810.1155/2018/30950963095096Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in KenyaOuko Abigael0Okoth Sheila1Amugune Nelson2Vesa Joutsjoki3School of Biological Sciences, University of Nairobi, P.O. Box 30197-00100, Nairobi, KenyaSchool of Biological Sciences, University of Nairobi, P.O. Box 30197-00100, Nairobi, KenyaSchool of Biological Sciences, University of Nairobi, P.O. Box 30197-00100, Nairobi, KenyaNatural Resources Institute Finland, FIN-31600, Jokioinen, FinlandIn this study, the possibility of sexual reproduction in sampled Aspergillus flavus strains was evaluated by assessing the distribution of mating type (MAT) genes, which are known to control sexual character among fungi, for two counties in Kenya. Forty-four isolates from Nandi and Makueni counties were genotyped by MAT using a multiplex polymerase chain reaction assay. The primer pair for the MAT1-1 amplified a 396 base pair (bp) fragment containing an α-box motif, and MAT1-2 primers targeted a 270 bp segment with a high mobility group protein. The MAT1-2 genes dominated in both regions although the frequency was higher in Nandi (75%) than in Makueni (54.17%). There were no MAT1-1 genes sampled in Nandi, and in Makueni their proportion was 15.91%. The percentage of isolates that amplified for both MAT genes in Makueni was 9.09%, while in Nandi it was 11.36%. Currently, use of aggressive aflatoxin non-producing A. flavus strains as biocontrol is the most promising preharvest aflatoxin control strategy in Kenya. However, we address the possibility of introduced biocontrol strains to breed with existing aflatoxin producing strains in nature, which could lead to the generation of A. flavus offspring capable of aflatoxin production while also being aggressive colonizers and possibly increasing the burden of aflatoxin exposure in food.http://dx.doi.org/10.1155/2018/3095096 |
| spellingShingle | Ouko Abigael Okoth Sheila Amugune Nelson Vesa Joutsjoki Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya Advances in Agriculture |
| title | Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya |
| title_full | Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya |
| title_fullStr | Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya |
| title_full_unstemmed | Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya |
| title_short | Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya |
| title_sort | characterization of mating type genes in aspergillus flavus populations from two locations in kenya |
| url | http://dx.doi.org/10.1155/2018/3095096 |
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