The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate
Genotoxic assessment of landfill leachate before and after biological treatment was conducted with two human cell lines (Me45 and NHDF) and Daphnia magna somatic cells. The alkali version of comet assay was used to examine genotoxicity of leachate by DNA strand breaks analysis and its repair dynamic...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/435239 |
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| _version_ | 1832546183031029760 |
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| author | Kamila Widziewicz Joanna Kalka Magdalena Skonieczna Paweł Madej |
| author_facet | Kamila Widziewicz Joanna Kalka Magdalena Skonieczna Paweł Madej |
| author_sort | Kamila Widziewicz |
| collection | DOAJ |
| description | Genotoxic assessment of landfill leachate before and after biological treatment was conducted with two human cell lines (Me45 and NHDF) and Daphnia magna somatic cells. The alkali version of comet assay was used to examine genotoxicity of leachate by DNA strand breaks analysis and its repair dynamics. The leachate samples were collected from Zabrze landfill, situated in the Upper Silesian Industrial District, Poland. Statistically significant differences (Kruskal-Wallice ANOVA rank model) were observed between DNA strand breaks in cells incubated with leachate before and after treatment (P<0.001). Nonparametric Friedman ANOVA confirmed time-reliable and concentration-reliable cells response to leachate concentration. Examinations of chemical properties showed a marked decrease in leachate parameters after treatment which correlate to reduced genotoxicity towards tested cells. Obtained results demonstrate that biological cotreatment of leachate together with municipal wastewater is an efficient method for its genotoxic potential reduction; however, treated leachate still possessed genotoxic character. |
| format | Article |
| id | doaj-art-27d740277f23406bbaa23d5d27916d41 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-27d740277f23406bbaa23d5d27916d412025-02-03T07:23:33ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/435239435239The Comet Assay for the Evaluation of Genotoxic Potential of Landfill LeachateKamila Widziewicz0Joanna Kalka1Magdalena Skonieczna2Paweł Madej3Institute of Water and Wastewater Engineering, Silesian University of Technology, Akademicka Street 2A, 44-100 Gliwice, PolandEnvironmental Biotechnology Department, Silesian University of Technology, Akademicka Street 2A, 44-100 Gliwice, PolandInstitute of Automatic Control, Silesian University of Technology, Akademicka Street 2A, 44-100 Gliwice, PolandEnvironmental Biotechnology Department, Silesian University of Technology, Akademicka Street 2A, 44-100 Gliwice, PolandGenotoxic assessment of landfill leachate before and after biological treatment was conducted with two human cell lines (Me45 and NHDF) and Daphnia magna somatic cells. The alkali version of comet assay was used to examine genotoxicity of leachate by DNA strand breaks analysis and its repair dynamics. The leachate samples were collected from Zabrze landfill, situated in the Upper Silesian Industrial District, Poland. Statistically significant differences (Kruskal-Wallice ANOVA rank model) were observed between DNA strand breaks in cells incubated with leachate before and after treatment (P<0.001). Nonparametric Friedman ANOVA confirmed time-reliable and concentration-reliable cells response to leachate concentration. Examinations of chemical properties showed a marked decrease in leachate parameters after treatment which correlate to reduced genotoxicity towards tested cells. Obtained results demonstrate that biological cotreatment of leachate together with municipal wastewater is an efficient method for its genotoxic potential reduction; however, treated leachate still possessed genotoxic character.http://dx.doi.org/10.1100/2012/435239 |
| spellingShingle | Kamila Widziewicz Joanna Kalka Magdalena Skonieczna Paweł Madej The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate The Scientific World Journal |
| title | The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate |
| title_full | The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate |
| title_fullStr | The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate |
| title_full_unstemmed | The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate |
| title_short | The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate |
| title_sort | comet assay for the evaluation of genotoxic potential of landfill leachate |
| url | http://dx.doi.org/10.1100/2012/435239 |
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