Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus
Nitric oxide and its derivative peroxynitrites are generated by host defense system to control bacterial infection. However certain Gram positive bacteria including Staphylococcus aureus possess a gene encoding nitric oxide synthase (SaNOS) in their chromosome. In this study it was determined that u...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2013/312146 |
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| author | Manisha Vaish Vineet K. Singh |
| author_facet | Manisha Vaish Vineet K. Singh |
| author_sort | Manisha Vaish |
| collection | DOAJ |
| description | Nitric oxide and its derivative peroxynitrites are generated by host defense system to control bacterial infection. However certain Gram positive bacteria including Staphylococcus aureus possess a gene encoding nitric oxide synthase (SaNOS) in their chromosome. In this study it was determined that under normal growth conditions, expression of SaNOS was highest during early exponential phase of the bacterial growth. In oxidative stress studies, deletion of SaNOS led to increased susceptibility of the mutant cells compared to wild-type S. aureus. While inhibition of SaNOS activity by the addition of L-NAME increased sensitivity of the wild-type S. aureus to oxidative stress, the addition of a nitric oxide donor, sodium nitroprusside, restored oxidative stress tolerance of the SaNOS mutant. The SaNOS mutant also showed reduced survival after phagocytosis by PMN cells with respect to wild-type S. aureus. |
| format | Article |
| id | doaj-art-27b1a3042c24442f9ed9bdc412b306b3 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-27b1a3042c24442f9ed9bdc412b306b32025-02-03T01:00:14ZengWileyInternational Journal of Microbiology1687-918X1687-91982013-01-01201310.1155/2013/312146312146Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureusManisha Vaish0Vineet K. Singh1Microbiology and Immunology, Kirksville College of Osteopathic Medicine, A.T. Still University of Health Sciences, 800 West Jefferson Street, Kirksville, MO 63501, USAMicrobiology and Immunology, Kirksville College of Osteopathic Medicine, A.T. Still University of Health Sciences, 800 West Jefferson Street, Kirksville, MO 63501, USANitric oxide and its derivative peroxynitrites are generated by host defense system to control bacterial infection. However certain Gram positive bacteria including Staphylococcus aureus possess a gene encoding nitric oxide synthase (SaNOS) in their chromosome. In this study it was determined that under normal growth conditions, expression of SaNOS was highest during early exponential phase of the bacterial growth. In oxidative stress studies, deletion of SaNOS led to increased susceptibility of the mutant cells compared to wild-type S. aureus. While inhibition of SaNOS activity by the addition of L-NAME increased sensitivity of the wild-type S. aureus to oxidative stress, the addition of a nitric oxide donor, sodium nitroprusside, restored oxidative stress tolerance of the SaNOS mutant. The SaNOS mutant also showed reduced survival after phagocytosis by PMN cells with respect to wild-type S. aureus.http://dx.doi.org/10.1155/2013/312146 |
| spellingShingle | Manisha Vaish Vineet K. Singh Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus International Journal of Microbiology |
| title | Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus |
| title_full | Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus |
| title_fullStr | Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus |
| title_full_unstemmed | Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus |
| title_short | Antioxidant Functions of Nitric Oxide Synthase in a Methicillin Sensitive Staphylococcus aureus |
| title_sort | antioxidant functions of nitric oxide synthase in a methicillin sensitive staphylococcus aureus |
| url | http://dx.doi.org/10.1155/2013/312146 |
| work_keys_str_mv | AT manishavaish antioxidantfunctionsofnitricoxidesynthaseinamethicillinsensitivestaphylococcusaureus AT vineetksingh antioxidantfunctionsofnitricoxidesynthaseinamethicillinsensitivestaphylococcusaureus |