miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMT
Reprogramming of the tumor immune microenvironment is a salient feature during metastasis in LUAD. miR-24-3p and KLF8, which are key regulators of the tumor immune microenvironment, had been proved to show metastasis-promoting property in LUAD. However, whether miR-24-3p could regulate LUAD metastas...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/4036047 |
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| _version_ | 1832547622366216192 |
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| author | Pengyu Jing Nianlin Xie Nan Zhao Ximing Zhu Pei Li Guizhou Gao Haizhou Dang Zhongping Gu |
| author_facet | Pengyu Jing Nianlin Xie Nan Zhao Ximing Zhu Pei Li Guizhou Gao Haizhou Dang Zhongping Gu |
| author_sort | Pengyu Jing |
| collection | DOAJ |
| description | Reprogramming of the tumor immune microenvironment is a salient feature during metastasis in LUAD. miR-24-3p and KLF8, which are key regulators of the tumor immune microenvironment, had been proved to show metastasis-promoting property in LUAD. However, whether miR-24-3p could regulate LUAD metastasis by targeting KLF8 remains unclear. This study explored the functions and mechanisms of miR-24-3p/KLF8 signaling in advanced LUAD. The expression level of miR-24-3p and KLF8 were tested in LUAD patients, and the corelation of miR-24-3p and KLF8 was evaluated. The interaction of miR-24-3p and KLF8 was demonstrated by luciferase reporter activity assay, in vitro migration and invasion studies, and in vivo metastatic studies. miR-24-3p level was downregulated in LUAD and negatively associated with KLF8 mRNA expression. miR-24-3p controls LUAD metastasis by directly targeting KLF8 and inducing Snail and E-cadherin expressions. Targeting the miR-24-3p/KLF8/EMT axis might be of great therapeutic value to advanced LUAD patients. |
| format | Article |
| id | doaj-art-27a5e7d6b1244125801d2f99f0b77955 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-27a5e7d6b1244125801d2f99f0b779552025-02-03T06:43:50ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/40360474036047miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMTPengyu Jing0Nianlin Xie1Nan Zhao2Ximing Zhu3Pei Li4Guizhou Gao5Haizhou Dang6Zhongping Gu7Department of Thoracic Surgery, The Second Affiliated Hospital, Air Force Medical University, Xi’an 710038, ChinaDepartment of Thoracic Surgery, The Second Affiliated Hospital, Air Force Medical University, Xi’an 710038, ChinaDepartment of Public Health, Xi’an Medical University, Xi’an 710021, ChinaDepartment of Thoracic Surgery, The Second Affiliated Hospital, Air Force Medical University, Xi’an 710038, ChinaDepartment of Thoracic Surgery, The Second Affiliated Hospital, Air Force Medical University, Xi’an 710038, ChinaDepartment of Thoracic Surgery, The Second Affiliated Hospital, Air Force Medical University, Xi’an 710038, ChinaDepartment of Thoracic Surgery, The Second Affiliated Hospital, Air Force Medical University, Xi’an 710038, ChinaDepartment of Thoracic Surgery, The Second Affiliated Hospital, Air Force Medical University, Xi’an 710038, ChinaReprogramming of the tumor immune microenvironment is a salient feature during metastasis in LUAD. miR-24-3p and KLF8, which are key regulators of the tumor immune microenvironment, had been proved to show metastasis-promoting property in LUAD. However, whether miR-24-3p could regulate LUAD metastasis by targeting KLF8 remains unclear. This study explored the functions and mechanisms of miR-24-3p/KLF8 signaling in advanced LUAD. The expression level of miR-24-3p and KLF8 were tested in LUAD patients, and the corelation of miR-24-3p and KLF8 was evaluated. The interaction of miR-24-3p and KLF8 was demonstrated by luciferase reporter activity assay, in vitro migration and invasion studies, and in vivo metastatic studies. miR-24-3p level was downregulated in LUAD and negatively associated with KLF8 mRNA expression. miR-24-3p controls LUAD metastasis by directly targeting KLF8 and inducing Snail and E-cadherin expressions. Targeting the miR-24-3p/KLF8/EMT axis might be of great therapeutic value to advanced LUAD patients.http://dx.doi.org/10.1155/2020/4036047 |
| spellingShingle | Pengyu Jing Nianlin Xie Nan Zhao Ximing Zhu Pei Li Guizhou Gao Haizhou Dang Zhongping Gu miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMT Journal of Immunology Research |
| title | miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMT |
| title_full | miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMT |
| title_fullStr | miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMT |
| title_full_unstemmed | miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMT |
| title_short | miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMT |
| title_sort | mir 24 3p klf8 signaling axis contributes to luad metastasis by regulating emt |
| url | http://dx.doi.org/10.1155/2020/4036047 |
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