Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India
A previous study from West Bengal documented very high rate of occult HBV infection (OBI) among the HBsAg negative blood donors. This study was aimed to characterize the OBI strains circulating among the blood donors and to estimate the risk associated with the prevailing viral variants/mutants. Blo...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/212704 |
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| author | Avik Biswas Rajesh Panigrahi Partha Kumar Chandra Arup Banerjee Sibnarayan Datta Manisha Pal Subhashish Chakraborty Prasun Bhattacharya Sekhar Chakrabarti Runu Chakravarty |
| author_facet | Avik Biswas Rajesh Panigrahi Partha Kumar Chandra Arup Banerjee Sibnarayan Datta Manisha Pal Subhashish Chakraborty Prasun Bhattacharya Sekhar Chakrabarti Runu Chakravarty |
| author_sort | Avik Biswas |
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| description | A previous study from West Bengal documented very high rate of occult HBV infection (OBI) among the HBsAg negative blood donors. This study was aimed to characterize the OBI strains circulating among the blood donors and to estimate the risk associated with the prevailing viral variants/mutants. Blood samples from 2195 voluntary blood donors were included in the study. HBsAg, HBeAg, anti-HBc, and anti-HBs statuses of the samples were done by ELISA based detection. PCR amplification and sequencing were done to determine HBV genotypes, basal core promoter (BCP), and precore (Pre-C) mutations. Among the study samples, 268 were anti-HBc positive/HBsAg negative, among which 65 (24.25%) were HBV DNA positive. Phylogenetic analysis revealed the presence of HBV/D (87.23%), HBV/A (8.51%), and HBV/C (4.26%) (P<0.0001).
HBV/D3 (65.85%) was the significantly prevalent subgenotype over HBV/D2 (26.83%) and HBV/D1 (7.31%) (P=0.0003). Considerable prevalence of differential BCP (1752C, 1753C, 1762T/1764A, 1753C+1762T/1764A, 1773C, and 1814C) and reverse transcriptase (rt) gene (rtI91L, rtL93P, rtS106C, rtR110G, rtN118T, rtS119T, rtY126H, rtG127W/R, rtC136R, and rtY158H) mutations was identified. Association of specific HBV subgenotypes with OBI was interesting and needs further study. Clinically relevant mutations were prevalent among the OBI strains which are of serious concern. |
| format | Article |
| id | doaj-art-27937c15b2f5406b8c2eebf85f2ab0ee |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-27937c15b2f5406b8c2eebf85f2ab0ee2025-02-03T01:12:30ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/212704212704Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern IndiaAvik Biswas0Rajesh Panigrahi1Partha Kumar Chandra2Arup Banerjee3Sibnarayan Datta4Manisha Pal5Subhashish Chakraborty6Prasun Bhattacharya7Sekhar Chakrabarti8Runu Chakravarty9ICMR Virus Unit, Kolkata, GB 4, 1st Floor, ID & BG Hospital Campus, 57 Dr. Suresh Chandra Banerjee Road, Kolkata 700010, IndiaICMR Virus Unit, Kolkata, GB 4, 1st Floor, ID & BG Hospital Campus, 57 Dr. Suresh Chandra Banerjee Road, Kolkata 700010, IndiaICMR Virus Unit, Kolkata, GB 4, 1st Floor, ID & BG Hospital Campus, 57 Dr. Suresh Chandra Banerjee Road, Kolkata 700010, IndiaICMR Virus Unit, Kolkata, GB 4, 1st Floor, ID & BG Hospital Campus, 57 Dr. Suresh Chandra Banerjee Road, Kolkata 700010, IndiaICMR Virus Unit, Kolkata, GB 4, 1st Floor, ID & BG Hospital Campus, 57 Dr. Suresh Chandra Banerjee Road, Kolkata 700010, IndiaDepartment of Statistics, University of Calcutta, Kolkata 700019, IndiaInstitute of Blood Transfusion Medicine and Immunohematology, Kolkata 700006, IndiaMedical College & Hospital, Kolkata 700073, West Bengal, IndiaNational Institute of Cholera and Enteric Diseases, Kolkata 700010, IndiaICMR Virus Unit, Kolkata, GB 4, 1st Floor, ID & BG Hospital Campus, 57 Dr. Suresh Chandra Banerjee Road, Kolkata 700010, IndiaA previous study from West Bengal documented very high rate of occult HBV infection (OBI) among the HBsAg negative blood donors. This study was aimed to characterize the OBI strains circulating among the blood donors and to estimate the risk associated with the prevailing viral variants/mutants. Blood samples from 2195 voluntary blood donors were included in the study. HBsAg, HBeAg, anti-HBc, and anti-HBs statuses of the samples were done by ELISA based detection. PCR amplification and sequencing were done to determine HBV genotypes, basal core promoter (BCP), and precore (Pre-C) mutations. Among the study samples, 268 were anti-HBc positive/HBsAg negative, among which 65 (24.25%) were HBV DNA positive. Phylogenetic analysis revealed the presence of HBV/D (87.23%), HBV/A (8.51%), and HBV/C (4.26%) (P<0.0001). HBV/D3 (65.85%) was the significantly prevalent subgenotype over HBV/D2 (26.83%) and HBV/D1 (7.31%) (P=0.0003). Considerable prevalence of differential BCP (1752C, 1753C, 1762T/1764A, 1753C+1762T/1764A, 1773C, and 1814C) and reverse transcriptase (rt) gene (rtI91L, rtL93P, rtS106C, rtR110G, rtN118T, rtS119T, rtY126H, rtG127W/R, rtC136R, and rtY158H) mutations was identified. Association of specific HBV subgenotypes with OBI was interesting and needs further study. Clinically relevant mutations were prevalent among the OBI strains which are of serious concern.http://dx.doi.org/10.1155/2013/212704 |
| spellingShingle | Avik Biswas Rajesh Panigrahi Partha Kumar Chandra Arup Banerjee Sibnarayan Datta Manisha Pal Subhashish Chakraborty Prasun Bhattacharya Sekhar Chakrabarti Runu Chakravarty Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India The Scientific World Journal |
| title | Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India |
| title_full | Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India |
| title_fullStr | Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India |
| title_full_unstemmed | Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India |
| title_short | Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India |
| title_sort | characterization of the occult hepatitis b virus variants circulating among the blood donors from eastern india |
| url | http://dx.doi.org/10.1155/2013/212704 |
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