The effect of diclofenac on proliferation and production of growth factors by endothelial cells (HMEC-1) under hypoxia and inflammatory conditions
Diclofenac belongs to non-steroidal anti-inflammatory drugs (NSAIDs) and non-selective COX inhibitors. The aim of this study was to examine the effect of diclofenac on endothelial cell proliferation under the influence of hypoxia or inflammatory conditions. Another goal was to check whether diclofen...
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Sciendo
2014-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | http://www.degruyter.com/view/j/acph.2014.64.issue-1/acph-2014-0006/acph-2014-0006.xml?format=INT |
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| author | Wiktorowska-Owczarek Anna |
| author_facet | Wiktorowska-Owczarek Anna |
| author_sort | Wiktorowska-Owczarek Anna |
| collection | DOAJ |
| description | Diclofenac belongs to non-steroidal anti-inflammatory drugs (NSAIDs) and non-selective COX inhibitors. The aim of this study was to examine the effect of diclofenac on endothelial cell proliferation under the influence of hypoxia or inflammatory conditions. Another goal was to check whether diclofenac modulates the secretion of angiogenic factors such as VEGF and bFGF in human microvascular endothelial cells (HMEC-1) in the presence of CoCl2 or lipopolysaccharide (LPS), which could influence the endothelial cells in an autocrine manner or other cells in a paracrine manner. HMEC-1 cells were treated with 0.1 and 0.3 mmol L-1 diclofenac in the presence of 100 μg mL-1 LPS or 200 μmol L-1 CoCl2. Diclofenac decreased cell viability under hypoxia and inflammatory conditions. The stimulation of bFGF secretion by LPS in microvascular endothelial cells (HMEC-1 cell) was attenuated by diclofenac. Diclofenac increased the secretion of VEGF induced by LPS and hypoxia. |
| format | Article |
| id | doaj-art-2784fe63ba5e437099b5ab5b74df94e3 |
| institution | Kabale University |
| issn | 1330-0075 1846-9558 |
| language | English |
| publishDate | 2014-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-2784fe63ba5e437099b5ab5b74df94e32025-02-02T15:28:09ZengSciendoActa Pharmaceutica1330-00751846-95582014-03-0164113113810.2478/acph-2014-0006acph-2014-0006The effect of diclofenac on proliferation and production of growth factors by endothelial cells (HMEC-1) under hypoxia and inflammatory conditionsWiktorowska-Owczarek Anna0Department of Pharmacology Chair of Pharmacology and Clinical Pharmacology Medical University of Lodz, PolandDiclofenac belongs to non-steroidal anti-inflammatory drugs (NSAIDs) and non-selective COX inhibitors. The aim of this study was to examine the effect of diclofenac on endothelial cell proliferation under the influence of hypoxia or inflammatory conditions. Another goal was to check whether diclofenac modulates the secretion of angiogenic factors such as VEGF and bFGF in human microvascular endothelial cells (HMEC-1) in the presence of CoCl2 or lipopolysaccharide (LPS), which could influence the endothelial cells in an autocrine manner or other cells in a paracrine manner. HMEC-1 cells were treated with 0.1 and 0.3 mmol L-1 diclofenac in the presence of 100 μg mL-1 LPS or 200 μmol L-1 CoCl2. Diclofenac decreased cell viability under hypoxia and inflammatory conditions. The stimulation of bFGF secretion by LPS in microvascular endothelial cells (HMEC-1 cell) was attenuated by diclofenac. Diclofenac increased the secretion of VEGF induced by LPS and hypoxia.http://www.degruyter.com/view/j/acph.2014.64.issue-1/acph-2014-0006/acph-2014-0006.xml?format=INTLPSVEGFbFGFdiclofenachypoxiaendothelial cells |
| spellingShingle | Wiktorowska-Owczarek Anna The effect of diclofenac on proliferation and production of growth factors by endothelial cells (HMEC-1) under hypoxia and inflammatory conditions Acta Pharmaceutica LPS VEGF bFGF diclofenac hypoxia endothelial cells |
| title | The effect of diclofenac on proliferation and production of growth factors by endothelial cells (HMEC-1) under hypoxia and inflammatory conditions |
| title_full | The effect of diclofenac on proliferation and production of growth factors by endothelial cells (HMEC-1) under hypoxia and inflammatory conditions |
| title_fullStr | The effect of diclofenac on proliferation and production of growth factors by endothelial cells (HMEC-1) under hypoxia and inflammatory conditions |
| title_full_unstemmed | The effect of diclofenac on proliferation and production of growth factors by endothelial cells (HMEC-1) under hypoxia and inflammatory conditions |
| title_short | The effect of diclofenac on proliferation and production of growth factors by endothelial cells (HMEC-1) under hypoxia and inflammatory conditions |
| title_sort | effect of diclofenac on proliferation and production of growth factors by endothelial cells hmec 1 under hypoxia and inflammatory conditions |
| topic | LPS VEGF bFGF diclofenac hypoxia endothelial cells |
| url | http://www.degruyter.com/view/j/acph.2014.64.issue-1/acph-2014-0006/acph-2014-0006.xml?format=INT |
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