Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia
Introduction. Müller glial cells typically activate to react to hypoxic tissue damage in several retinal diseases. We evaluated the in vitro response to a hypoxia-mimicking stimulus on the expression of a set of genes, known to contribute to eye morphogenesis and cell differentiation. Materials and...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2021/6265553 |
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| author | Claudio Azzolini Simone Donati Giovanni Micheloni Vittoria Moretti Roberto Valli Francesco Acquati Lucy Costantino Fulvio Ferrara Davide Borroni Elias Premi Francesco Testa Francesca Simonelli Giovanni Porta |
| author_facet | Claudio Azzolini Simone Donati Giovanni Micheloni Vittoria Moretti Roberto Valli Francesco Acquati Lucy Costantino Fulvio Ferrara Davide Borroni Elias Premi Francesco Testa Francesca Simonelli Giovanni Porta |
| author_sort | Claudio Azzolini |
| collection | DOAJ |
| description | Introduction. Müller glial cells typically activate to react to hypoxic tissue damage in several retinal diseases. We evaluated the in vitro response to a hypoxia-mimicking stimulus on the expression of a set of genes, known to contribute to eye morphogenesis and cell differentiation. Materials and Methods. A MIO-M1 Müller cell line was cultured in a hypoxia-mimicking environment by the addition of cobalt chloride to the culture medium, followed by a recovery time in which we mimic restoration from the hypoxic insult. The HIF-1α protein and VEGF-A gene expression were quantified to verify the induction of a hypoxia-like state. Results. Among the genes under study, we did not observe any difference in the expression levels of Otx1 and Otx2 during treatment; conversely, Otx1 was overexpressed during recovery steps. The VEGF-A gene was strongly upregulated at both the CoCl2 and recovery time points. The transactivated isoform (TA) of the TP73 gene showed an overexpression in long-term exposure to the hypoxic stimulus with a further increase after recovery. Discussion. Our molecular analysis is able to describe the activation of a set of genes, never before described, that can drive the response to a hypoxia-like status. The improved comprehension of these cellular events will be useful for designing new therapeutical approaches for retinal pathologies. |
| format | Article |
| id | doaj-art-27730412e2af4bcf917e2045c4ded3ea |
| institution | Kabale University |
| issn | 2090-0058 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-27730412e2af4bcf917e2045c4ded3ea2025-02-03T06:45:28ZengWileyJournal of Ophthalmology2090-00582021-01-01202110.1155/2021/6265553Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal HypoxiaClaudio Azzolini0Simone Donati1Giovanni Micheloni2Vittoria Moretti3Roberto Valli4Francesco Acquati5Lucy Costantino6Fulvio Ferrara7Davide Borroni8Elias Premi9Francesco Testa10Francesca Simonelli11Giovanni Porta12Department of Medicine and SurgeryDepartment of Medicine and SurgeryGenomic Medicine Research CenterGenomic Medicine Research CenterGenomic Medicine Research CenterGenomic Medicine Research CenterDepartment of Molecular GeneticsDepartment of Molecular GeneticsFondazione Banca Degli Occhi Del Veneto OnlusOphthalmology UnitEye ClinicEye ClinicGenomic Medicine Research CenterIntroduction. Müller glial cells typically activate to react to hypoxic tissue damage in several retinal diseases. We evaluated the in vitro response to a hypoxia-mimicking stimulus on the expression of a set of genes, known to contribute to eye morphogenesis and cell differentiation. Materials and Methods. A MIO-M1 Müller cell line was cultured in a hypoxia-mimicking environment by the addition of cobalt chloride to the culture medium, followed by a recovery time in which we mimic restoration from the hypoxic insult. The HIF-1α protein and VEGF-A gene expression were quantified to verify the induction of a hypoxia-like state. Results. Among the genes under study, we did not observe any difference in the expression levels of Otx1 and Otx2 during treatment; conversely, Otx1 was overexpressed during recovery steps. The VEGF-A gene was strongly upregulated at both the CoCl2 and recovery time points. The transactivated isoform (TA) of the TP73 gene showed an overexpression in long-term exposure to the hypoxic stimulus with a further increase after recovery. Discussion. Our molecular analysis is able to describe the activation of a set of genes, never before described, that can drive the response to a hypoxia-like status. The improved comprehension of these cellular events will be useful for designing new therapeutical approaches for retinal pathologies.http://dx.doi.org/10.1155/2021/6265553 |
| spellingShingle | Claudio Azzolini Simone Donati Giovanni Micheloni Vittoria Moretti Roberto Valli Francesco Acquati Lucy Costantino Fulvio Ferrara Davide Borroni Elias Premi Francesco Testa Francesca Simonelli Giovanni Porta Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia Journal of Ophthalmology |
| title | Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia |
| title_full | Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia |
| title_fullStr | Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia |
| title_full_unstemmed | Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia |
| title_short | Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia |
| title_sort | expression of otx genes in muller cells using an in vitro experimental model of retinal hypoxia |
| url | http://dx.doi.org/10.1155/2021/6265553 |
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