Optimization of Conditions for Production of Soluble <i>E. coli</i> Poly(A)-Polymerase for Biotechnological Applications
Poly(A) polymerase (PAP 1) from <i>Escherichia coli</i> is the primary enzyme responsible for synthesizing poly(A) tails on RNA molecules, signaling RNA degradation in bacterial cells. In vitro, PAP 1 is used to prepare libraries for RNAseq and to produce mRNA vaccines. However, <i>...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
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MDPI AG
2025-01-01
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Series: | Biology |
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Online Access: | https://www.mdpi.com/2079-7737/14/1/48 |
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