Salivary Protein Profile and Food Intake: A Dietary Pattern Analysis
Saliva research has gained interest due to its potential as a source of biomarkers. One of the factors inducing changes in saliva, in the short term, is food intake, and evidence exist about changes in salivary proteome induced by some food components. Since this topic of research is in its early st...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2021/6629951 |
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| author | Teresa Louro Carla Simões Wilmara Lima Laura Carreira Paula Midori Castelo Henrique Luis Pedro Moreira Elsa Lamy |
| author_facet | Teresa Louro Carla Simões Wilmara Lima Laura Carreira Paula Midori Castelo Henrique Luis Pedro Moreira Elsa Lamy |
| author_sort | Teresa Louro |
| collection | DOAJ |
| description | Saliva research has gained interest due to its potential as a source of biomarkers. One of the factors inducing changes in saliva, in the short term, is food intake, and evidence exist about changes in salivary proteome induced by some food components. Since this topic of research is in its early stages, it was hypothesized that saliva protein composition could be associated with different levels of adherence to dietary patterns that contain higher amounts of plant products. The aim of the present study was to test this hypothesis, in adults, by comparing salivary protein electrophoretic profiles of individuals with different diet characteristics, particularly dietary patterns (DP) that exhibit different proportions of animal and plant-based products. Dietary habits were assessed in 122 adults (61 from each sex, with ages ranging from 20 to 59 years) using Food Frequency Questionnaires. To identify the dietary patterns, a principal component analysis was used. Individual’s non-stimulated saliva was evaluated for flow rate, pH, protein concentration, α-amylase activity, and electrophoretic protein profiles. Seven dietary patterns (DP) were identified. Salivary amylase enzymatic activity was positively associated with animal-based and starchy foods DP, and with plant-based fatty foods without wine DP. At the same time, protein bands containing amylase and type S cystatins were positively associated with the cheese/yoghurt and wine DP. Our results support the association of salivary proteomics and different dietary patterns and highlight the need of considering food consumption habits in studies using saliva, since this is a factor associated with variations in the composition of this fluid. |
| format | Article |
| id | doaj-art-270068a07dcd47d188874266eb520490 |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-270068a07dcd47d188874266eb5204902025-02-03T06:47:02ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322021-01-01202110.1155/2021/66299516629951Salivary Protein Profile and Food Intake: A Dietary Pattern AnalysisTeresa Louro0Carla Simões1Wilmara Lima2Laura Carreira3Paula Midori Castelo4Henrique Luis5Pedro Moreira6Elsa Lamy7MED (Mediterranean Institute for Agriculture, Environment and Development), Institute for Advanced Studies and Research (IIFA)IIFA–Instituto de Investigação e Formação Avançada, University of Évora, Évora, PortugalMED (Mediterranean Institute for Agriculture, Environment and Development), Institute for Advanced Studies and Research (IIFA)IIFA–Instituto de Investigação e Formação Avançada, University of Évora, Évora, PortugalStudent from the Department of Chemistry, University of Évora, Évora, PortugalStudent from the Department of Chemistry, University of Évora, Évora, PortugalDept. Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, BrazilSchool of Dental Medicine, University of Lisbon, Research Unit of Oral and Biomedical Sciences (UICOB), Lisbon, PortugalFaculdade de Ciências da Nutrição e Alimentação da Universidade Do Porto, Porto 4150-180, PortugalMED (Mediterranean Institute for Agriculture, Environment and Development), Institute for Advanced Studies and Research (IIFA)IIFA–Instituto de Investigação e Formação Avançada, University of Évora, Évora, PortugalSaliva research has gained interest due to its potential as a source of biomarkers. One of the factors inducing changes in saliva, in the short term, is food intake, and evidence exist about changes in salivary proteome induced by some food components. Since this topic of research is in its early stages, it was hypothesized that saliva protein composition could be associated with different levels of adherence to dietary patterns that contain higher amounts of plant products. The aim of the present study was to test this hypothesis, in adults, by comparing salivary protein electrophoretic profiles of individuals with different diet characteristics, particularly dietary patterns (DP) that exhibit different proportions of animal and plant-based products. Dietary habits were assessed in 122 adults (61 from each sex, with ages ranging from 20 to 59 years) using Food Frequency Questionnaires. To identify the dietary patterns, a principal component analysis was used. Individual’s non-stimulated saliva was evaluated for flow rate, pH, protein concentration, α-amylase activity, and electrophoretic protein profiles. Seven dietary patterns (DP) were identified. Salivary amylase enzymatic activity was positively associated with animal-based and starchy foods DP, and with plant-based fatty foods without wine DP. At the same time, protein bands containing amylase and type S cystatins were positively associated with the cheese/yoghurt and wine DP. Our results support the association of salivary proteomics and different dietary patterns and highlight the need of considering food consumption habits in studies using saliva, since this is a factor associated with variations in the composition of this fluid.http://dx.doi.org/10.1155/2021/6629951 |
| spellingShingle | Teresa Louro Carla Simões Wilmara Lima Laura Carreira Paula Midori Castelo Henrique Luis Pedro Moreira Elsa Lamy Salivary Protein Profile and Food Intake: A Dietary Pattern Analysis Journal of Nutrition and Metabolism |
| title | Salivary Protein Profile and Food Intake: A Dietary Pattern Analysis |
| title_full | Salivary Protein Profile and Food Intake: A Dietary Pattern Analysis |
| title_fullStr | Salivary Protein Profile and Food Intake: A Dietary Pattern Analysis |
| title_full_unstemmed | Salivary Protein Profile and Food Intake: A Dietary Pattern Analysis |
| title_short | Salivary Protein Profile and Food Intake: A Dietary Pattern Analysis |
| title_sort | salivary protein profile and food intake a dietary pattern analysis |
| url | http://dx.doi.org/10.1155/2021/6629951 |
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