One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal
A purification technique to obtain keratinolytic proteases produced by Bacillus sp. P45 in a medium containing chicken feather meal as substrate is presented. The experiments were carried out in a dead-end ultrafiltration unit, and the influence of the membrane cutoff, pH of enzymatic extract, and o...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Chemical Engineering |
| Online Access: | http://dx.doi.org/10.1155/2018/6729490 |
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| author | Juliana R. Machado Emanuelle E. Severo Janaina M. G. de Oliveira Joana da C. Ores Adriano Brandelli Susana J. Kalil |
| author_facet | Juliana R. Machado Emanuelle E. Severo Janaina M. G. de Oliveira Joana da C. Ores Adriano Brandelli Susana J. Kalil |
| author_sort | Juliana R. Machado |
| collection | DOAJ |
| description | A purification technique to obtain keratinolytic proteases produced by Bacillus sp. P45 in a medium containing chicken feather meal as substrate is presented. The experiments were carried out in a dead-end ultrafiltration unit, and the influence of the membrane cutoff, pH of enzymatic extract, and operating pressure on the purification of keratinase were studied. The one-step ultrafiltration process with the membrane molecular mass cutoff of 10 kDa at pH 8.0 and operating pressure of 0.147 MPa showed an enzyme recovery of 87.8% and a 4.1-fold purification factor. It is showed that ultrafiltration could be potentially used in the purification of keratinases. |
| format | Article |
| id | doaj-art-26d8de3e8fd7481eada57f618c834cb6 |
| institution | Kabale University |
| issn | 1687-806X 1687-8078 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Chemical Engineering |
| spelling | doaj-art-26d8de3e8fd7481eada57f618c834cb62025-02-03T05:49:46ZengWileyInternational Journal of Chemical Engineering1687-806X1687-80782018-01-01201810.1155/2018/67294906729490One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather MealJuliana R. Machado0Emanuelle E. Severo1Janaina M. G. de Oliveira2Joana da C. Ores3Adriano Brandelli4Susana J. Kalil5Escola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 474, 96203900 Rio Grande, RS, BrazilEscola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 474, 96203900 Rio Grande, RS, BrazilEscola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 474, 96203900 Rio Grande, RS, BrazilEscola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 474, 96203900 Rio Grande, RS, BrazilLaboratório de Bioquímica e Microbiologia Aplicada, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, RS, BrazilEscola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 474, 96203900 Rio Grande, RS, BrazilA purification technique to obtain keratinolytic proteases produced by Bacillus sp. P45 in a medium containing chicken feather meal as substrate is presented. The experiments were carried out in a dead-end ultrafiltration unit, and the influence of the membrane cutoff, pH of enzymatic extract, and operating pressure on the purification of keratinase were studied. The one-step ultrafiltration process with the membrane molecular mass cutoff of 10 kDa at pH 8.0 and operating pressure of 0.147 MPa showed an enzyme recovery of 87.8% and a 4.1-fold purification factor. It is showed that ultrafiltration could be potentially used in the purification of keratinases.http://dx.doi.org/10.1155/2018/6729490 |
| spellingShingle | Juliana R. Machado Emanuelle E. Severo Janaina M. G. de Oliveira Joana da C. Ores Adriano Brandelli Susana J. Kalil One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal International Journal of Chemical Engineering |
| title | One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal |
| title_full | One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal |
| title_fullStr | One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal |
| title_full_unstemmed | One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal |
| title_short | One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal |
| title_sort | one step ultrafiltration process for separation and purification of a keratinolytic protease produced with feather meal |
| url | http://dx.doi.org/10.1155/2018/6729490 |
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