UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry Fruit

Mulberry (Morus alba L.) has been used in East Asia (Korea, China, and Japan) as a medicine because of its various pharmacological effects including the excellent antioxidant properties of its fruit. This study analyzed extracts from 12 varieties of Korean mulberry fruit for flavonoids using ultrahi...

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Main Authors: O-Chul Kwon, Wan-Taek Ju, Hyun-Bok Kim, Gyoo-Byung Sung, Yong-Soon Kim
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Journal of Food Quality
Online Access:http://dx.doi.org/10.1155/2019/1528917
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author O-Chul Kwon
Wan-Taek Ju
Hyun-Bok Kim
Gyoo-Byung Sung
Yong-Soon Kim
author_facet O-Chul Kwon
Wan-Taek Ju
Hyun-Bok Kim
Gyoo-Byung Sung
Yong-Soon Kim
author_sort O-Chul Kwon
collection DOAJ
description Mulberry (Morus alba L.) has been used in East Asia (Korea, China, and Japan) as a medicine because of its various pharmacological effects including the excellent antioxidant properties of its fruit. This study analyzed extracts from 12 varieties of Korean mulberry fruit for flavonoids using ultrahigh-performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS). Six quercetin derivatives were identified by mass spectrometry (MS) based on the [quercetin + H]+ ion (m/z 303), while four kaempferol derivatives were identified based on the [kaempferol + H]+ ion (m/z 287). Two new compounds (morkotin A and morkotin C, quercetin derivatives) were identified for the first time in mulberry fruit. The total flavonoid contents of the mulberry fruits ranged from 35.0 ± 2.3 mg/100 g DW in the Baek Ok Wang variety (white mulberry) to 119.9 ± 7.0 mg/100 g DW in the Dae Shim variety. This study has, for the first time, evaluated the flavonoid chromatographic profiles of 12 varieties of Korean mulberry fruits in a following quali-quantitative approach, which will contribute to improved utilization of these fruits as health foods.
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language English
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publisher Wiley
record_format Article
series Journal of Food Quality
spelling doaj-art-26d51f222cb34aee8e44e7c26f06f08f2025-02-03T05:58:39ZengWileyJournal of Food Quality0146-94281745-45572019-01-01201910.1155/2019/15289171528917UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry FruitO-Chul Kwon0Wan-Taek Ju1Hyun-Bok Kim2Gyoo-Byung Sung3Yong-Soon Kim4National Institute of Agricultural Sciences, Rural Development Administration, Wanju‐gun 55365, Republic of KoreaNational Institute of Agricultural Sciences, Rural Development Administration, Wanju‐gun 55365, Republic of KoreaNational Institute of Agricultural Sciences, Rural Development Administration, Wanju‐gun 55365, Republic of KoreaNational Institute of Agricultural Sciences, Rural Development Administration, Wanju‐gun 55365, Republic of KoreaNational Institute of Agricultural Sciences, Rural Development Administration, Wanju‐gun 55365, Republic of KoreaMulberry (Morus alba L.) has been used in East Asia (Korea, China, and Japan) as a medicine because of its various pharmacological effects including the excellent antioxidant properties of its fruit. This study analyzed extracts from 12 varieties of Korean mulberry fruit for flavonoids using ultrahigh-performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS). Six quercetin derivatives were identified by mass spectrometry (MS) based on the [quercetin + H]+ ion (m/z 303), while four kaempferol derivatives were identified based on the [kaempferol + H]+ ion (m/z 287). Two new compounds (morkotin A and morkotin C, quercetin derivatives) were identified for the first time in mulberry fruit. The total flavonoid contents of the mulberry fruits ranged from 35.0 ± 2.3 mg/100 g DW in the Baek Ok Wang variety (white mulberry) to 119.9 ± 7.0 mg/100 g DW in the Dae Shim variety. This study has, for the first time, evaluated the flavonoid chromatographic profiles of 12 varieties of Korean mulberry fruits in a following quali-quantitative approach, which will contribute to improved utilization of these fruits as health foods.http://dx.doi.org/10.1155/2019/1528917
spellingShingle O-Chul Kwon
Wan-Taek Ju
Hyun-Bok Kim
Gyoo-Byung Sung
Yong-Soon Kim
UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry Fruit
Journal of Food Quality
title UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry Fruit
title_full UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry Fruit
title_fullStr UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry Fruit
title_full_unstemmed UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry Fruit
title_short UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry Fruit
title_sort uplc dad qtof ms analysis of flavonoids from 12 varieties of korean mulberry fruit
url http://dx.doi.org/10.1155/2019/1528917
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