Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach
Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimenta...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/765909 |
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| author | Daniel V. Oliveira Tomé S. Silva Odete D. Cordeiro Sofia I. Cavaco Dina C. Simes |
| author_facet | Daniel V. Oliveira Tomé S. Silva Odete D. Cordeiro Sofia I. Cavaco Dina C. Simes |
| author_sort | Daniel V. Oliveira |
| collection | DOAJ |
| description | Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications. |
| format | Article |
| id | doaj-art-268f9db12bae43d3b6841e9008e51972 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-268f9db12bae43d3b6841e9008e519722025-02-03T05:43:49ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/765909765909Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic ApproachDaniel V. Oliveira0Tomé S. Silva1Odete D. Cordeiro2Sofia I. Cavaco3Dina C. Simes4Center of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, PortugalCenter of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, PortugalCenter of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, PortugalCenter of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, PortugalCenter of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, PortugalNacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications.http://dx.doi.org/10.1100/2012/765909 |
| spellingShingle | Daniel V. Oliveira Tomé S. Silva Odete D. Cordeiro Sofia I. Cavaco Dina C. Simes Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach The Scientific World Journal |
| title | Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach |
| title_full | Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach |
| title_fullStr | Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach |
| title_full_unstemmed | Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach |
| title_short | Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach |
| title_sort | identification of proteins with potential osteogenic activity present in the water soluble matrix proteins from crassostrea gigas nacre using a proteomic approach |
| url | http://dx.doi.org/10.1100/2012/765909 |
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