Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL <i>Escherichia coli</i>
<b>Background/Objectives:</b> Bacteriophage therapy represents a promising strategy to combat multidrug-resistant pathogens, such as <i>Escherichia coli</i>. In this study, we explored the effects of a bacteriophage infection on an Extended Spectrum Beta-Lactamase (ESBL) posi...
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| Format: | Article |
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MDPI AG
2025-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/14/1/76 |
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| author | Árpád Rózsa László Orosz Nikoletta Szemerédi Gabriella Spengler Gábor Kecskeméti Otília Vágó Károly Péter Sárvári Diana Szabó Zoltán Szabó Katalin Burián Dezső Péter Virok |
| author_facet | Árpád Rózsa László Orosz Nikoletta Szemerédi Gabriella Spengler Gábor Kecskeméti Otília Vágó Károly Péter Sárvári Diana Szabó Zoltán Szabó Katalin Burián Dezső Péter Virok |
| author_sort | Árpád Rózsa |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Bacteriophage therapy represents a promising strategy to combat multidrug-resistant pathogens, such as <i>Escherichia coli</i>. In this study, we explored the effects of a bacteriophage infection on an Extended Spectrum Beta-Lactamase (ESBL) positive <i>E. coli</i> isolate. <b>Methods:</b> We used next generation sequencing, proteomics and phenotypic screens to investigate the effect of bacteriophage infections on <i>E. coli</i> metabolism and resistance phenotypes. <b>Results:</b> The bacteriophage infection led to notable alterations in colony morphology, indicating profound changes in bacterial metabolism. Proteomic analysis revealed significant shifts in protein expression, with 65 proteins upregulated and 246 downregulated post-infection. The downregulated proteins were involved in various metabolic pathways, including nucleic acid, protein and lipid metabolism, and iron acquisition. Bacteriophage treatment also led to increased bacterial membrane permeability. Altogether, these alterations in bacterial metabolism and membrane permeability may lead to a general reduction in antibiotic resistance. Indeed, the bacteriophage-infected <i>E. coli</i> exhibited increased sensitivity to various classes of antibiotics, including beta-lactams, fluoroquinolones, trimethoprim-sulfamethoxazole, and aminoglycosides. <b>Conclusions:</b> Our findings highlight the potential of bacteriophage therapy as an adjunct to existing antibiotics, enhancing their efficacy against resistant strains. |
| format | Article |
| id | doaj-art-267a03f2d96a438e896c39aa8d9cf8c4 |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-267a03f2d96a438e896c39aa8d9cf8c42025-01-24T13:18:52ZengMDPI AGAntibiotics2079-63822025-01-011417610.3390/antibiotics14010076Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL <i>Escherichia coli</i>Árpád Rózsa0László Orosz1Nikoletta Szemerédi2Gabriella Spengler3Gábor Kecskeméti4Otília Vágó5Károly Péter Sárvári6Diana Szabó7Zoltán Szabó8Katalin Burián9Dezső Péter Virok10Pándy Kálmán County Hospital, Semmelweis Str. 1, H-5700 Gyula, HungaryDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis Str. 6, H-6725 Szeged, HungaryDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis Str. 6, H-6725 Szeged, HungaryDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis Str. 6, H-6725 Szeged, HungaryDepartment of Medical Chemistry, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Dóm Sq. 8, H-6720 Szeged, HungaryPándy Kálmán County Hospital, Semmelweis Str. 1, H-5700 Gyula, HungaryDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis Str. 6, H-6725 Szeged, HungaryDepartment of Oto-Rhino-Laryngology and Head & Neck Surgery, University of Szeged, Tisza Lajos Str. 111, H-6724 Szeged, HungaryDepartment of Medical Chemistry, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Dóm Sq. 8, H-6720 Szeged, HungaryDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis Str. 6, H-6725 Szeged, HungaryDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis Str. 6, H-6725 Szeged, Hungary<b>Background/Objectives:</b> Bacteriophage therapy represents a promising strategy to combat multidrug-resistant pathogens, such as <i>Escherichia coli</i>. In this study, we explored the effects of a bacteriophage infection on an Extended Spectrum Beta-Lactamase (ESBL) positive <i>E. coli</i> isolate. <b>Methods:</b> We used next generation sequencing, proteomics and phenotypic screens to investigate the effect of bacteriophage infections on <i>E. coli</i> metabolism and resistance phenotypes. <b>Results:</b> The bacteriophage infection led to notable alterations in colony morphology, indicating profound changes in bacterial metabolism. Proteomic analysis revealed significant shifts in protein expression, with 65 proteins upregulated and 246 downregulated post-infection. The downregulated proteins were involved in various metabolic pathways, including nucleic acid, protein and lipid metabolism, and iron acquisition. Bacteriophage treatment also led to increased bacterial membrane permeability. Altogether, these alterations in bacterial metabolism and membrane permeability may lead to a general reduction in antibiotic resistance. Indeed, the bacteriophage-infected <i>E. coli</i> exhibited increased sensitivity to various classes of antibiotics, including beta-lactams, fluoroquinolones, trimethoprim-sulfamethoxazole, and aminoglycosides. <b>Conclusions:</b> Our findings highlight the potential of bacteriophage therapy as an adjunct to existing antibiotics, enhancing their efficacy against resistant strains.https://www.mdpi.com/2079-6382/14/1/76bacteriophagecocktailantibioticresistance<i>Escherichia</i>siderophore |
| spellingShingle | Árpád Rózsa László Orosz Nikoletta Szemerédi Gabriella Spengler Gábor Kecskeméti Otília Vágó Károly Péter Sárvári Diana Szabó Zoltán Szabó Katalin Burián Dezső Péter Virok Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL <i>Escherichia coli</i> Antibiotics bacteriophage cocktail antibiotic resistance <i>Escherichia</i> siderophore |
| title | Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL <i>Escherichia coli</i> |
| title_full | Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL <i>Escherichia coli</i> |
| title_fullStr | Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL <i>Escherichia coli</i> |
| title_full_unstemmed | Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL <i>Escherichia coli</i> |
| title_short | Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL <i>Escherichia coli</i> |
| title_sort | bacteriophage treatment induces phenotype switching and alters antibiotic resistance of esbl i escherichia coli i |
| topic | bacteriophage cocktail antibiotic resistance <i>Escherichia</i> siderophore |
| url | https://www.mdpi.com/2079-6382/14/1/76 |
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