Tailoring and characterization of bioactive graft material for alveolar bone preservation and regeneration in fresh extraction sockets of dog model
Abstract The objective of this study was to tailor an osteoinductive scaffold for alveolar bone regeneration and around immediately placed implants in extraction sockets of dogs. Tailored amorphous multiporous bioactive glass (TAMP -BG) was prepared and characterized for bioactivity and response of...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86408-x |
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| author | Ayat Hamdy Manal Saad Rania ElBackly Samir Nouh Himanshu Jain Mona Marei |
| author_facet | Ayat Hamdy Manal Saad Rania ElBackly Samir Nouh Himanshu Jain Mona Marei |
| author_sort | Ayat Hamdy |
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| description | Abstract The objective of this study was to tailor an osteoinductive scaffold for alveolar bone regeneration and around immediately placed implants in extraction sockets of dogs. Tailored amorphous multiporous bioactive glass (TAMP -BG) was prepared and characterized for bioactivity and response of human alveolar bone marrow mesenchymal stem cells (hABMSCs). Extraction sockets of twenty-two male mongrel dogs received TAMP-BG in the right side around implant in the distal socket of the mandibular fourth premolar (P4), while the adjacent empty mesial socket of the same tooth was filled with the same graft. Autologous bone chips were used in the left side. Animals were euthanized at 1, 2, 4, 8 &12 weeks for histological and SEM analyses. SEM/EDX, FTIR and XRD analyses displayed formation of a bioactive hydroxycarbonated apatite layer. TAMP-BG significantly increased proliferation and migration of hABMSCs. Histologically, at the 1st week, advanced bone healing was shown in TAMP-BG group. At four weeks bone density was significantly higher forTAMP-BG (92.71% ± 1.71) versus control (62.92% ± 2.02) (P = 0.0001).At eight weeks, significant increase in width of buccal plate and height of lingual bony plate was observedfor TAMP-BG grafted implant.The socket orifice width significantly decreased for autologous bone from 1 to 12 weeks (P < 0.001), while it significantly increased for TAMP-BG (P = 0.03). We conclude that TAMP-BG can provide a preservative dynamic microenvironment following extraction up to three months which can be attributed to its unique physico-chemical characteristics. |
| format | Article |
| id | doaj-art-2653b38fdbb44826b688d7108830ca1c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Scientific Reports |
| spelling | doaj-art-2653b38fdbb44826b688d7108830ca1c2025-02-02T12:24:23ZengNature PortfolioScientific Reports2045-23222025-01-0115111610.1038/s41598-025-86408-xTailoring and characterization of bioactive graft material for alveolar bone preservation and regeneration in fresh extraction sockets of dog modelAyat Hamdy0Manal Saad1Rania ElBackly2Samir Nouh3Himanshu Jain4Mona Marei5Tissue Engineering Laboratories, Faculty of Dentistry, Alexandria UniversityTissue Engineering Laboratories, Faculty of Dentistry, Alexandria UniversityTissue Engineering Laboratories, Faculty of Dentistry, Alexandria UniversityTissue Engineering Laboratories, Faculty of Dentistry, Alexandria UniversityInstitute for Functional Materials and Devices (I-FMD), P.C. Rossin College of Engineering and Applied Science, Lehigh UniversityTissue Engineering Laboratories, Faculty of Dentistry, Alexandria UniversityAbstract The objective of this study was to tailor an osteoinductive scaffold for alveolar bone regeneration and around immediately placed implants in extraction sockets of dogs. Tailored amorphous multiporous bioactive glass (TAMP -BG) was prepared and characterized for bioactivity and response of human alveolar bone marrow mesenchymal stem cells (hABMSCs). Extraction sockets of twenty-two male mongrel dogs received TAMP-BG in the right side around implant in the distal socket of the mandibular fourth premolar (P4), while the adjacent empty mesial socket of the same tooth was filled with the same graft. Autologous bone chips were used in the left side. Animals were euthanized at 1, 2, 4, 8 &12 weeks for histological and SEM analyses. SEM/EDX, FTIR and XRD analyses displayed formation of a bioactive hydroxycarbonated apatite layer. TAMP-BG significantly increased proliferation and migration of hABMSCs. Histologically, at the 1st week, advanced bone healing was shown in TAMP-BG group. At four weeks bone density was significantly higher forTAMP-BG (92.71% ± 1.71) versus control (62.92% ± 2.02) (P = 0.0001).At eight weeks, significant increase in width of buccal plate and height of lingual bony plate was observedfor TAMP-BG grafted implant.The socket orifice width significantly decreased for autologous bone from 1 to 12 weeks (P < 0.001), while it significantly increased for TAMP-BG (P = 0.03). We conclude that TAMP-BG can provide a preservative dynamic microenvironment following extraction up to three months which can be attributed to its unique physico-chemical characteristics.https://doi.org/10.1038/s41598-025-86408-xBioactive glassDynamic tissue regenerationAlveolar bone defectMulti-porous scaffoldDegradable biomaterialsOsseointegration |
| spellingShingle | Ayat Hamdy Manal Saad Rania ElBackly Samir Nouh Himanshu Jain Mona Marei Tailoring and characterization of bioactive graft material for alveolar bone preservation and regeneration in fresh extraction sockets of dog model Scientific Reports Bioactive glass Dynamic tissue regeneration Alveolar bone defect Multi-porous scaffold Degradable biomaterials Osseointegration |
| title | Tailoring and characterization of bioactive graft material for alveolar bone preservation and regeneration in fresh extraction sockets of dog model |
| title_full | Tailoring and characterization of bioactive graft material for alveolar bone preservation and regeneration in fresh extraction sockets of dog model |
| title_fullStr | Tailoring and characterization of bioactive graft material for alveolar bone preservation and regeneration in fresh extraction sockets of dog model |
| title_full_unstemmed | Tailoring and characterization of bioactive graft material for alveolar bone preservation and regeneration in fresh extraction sockets of dog model |
| title_short | Tailoring and characterization of bioactive graft material for alveolar bone preservation and regeneration in fresh extraction sockets of dog model |
| title_sort | tailoring and characterization of bioactive graft material for alveolar bone preservation and regeneration in fresh extraction sockets of dog model |
| topic | Bioactive glass Dynamic tissue regeneration Alveolar bone defect Multi-porous scaffold Degradable biomaterials Osseointegration |
| url | https://doi.org/10.1038/s41598-025-86408-x |
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