Biogenic Synthesis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial Activity
The spread of infectious diseases and the increase in the drug resistance among microbes has forced the researchers to synthesize biologically active nanoparticles. Improvement of the ecofriendly procedure for the synthesis of nanoparticles is growing day-by-day in the field of nanobiotechnology. In...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2018/7879403 |
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| author | Anju Arya Khushbu Gupta Tejpal Singh Chundawat Dipti Vaya |
| author_facet | Anju Arya Khushbu Gupta Tejpal Singh Chundawat Dipti Vaya |
| author_sort | Anju Arya |
| collection | DOAJ |
| description | The spread of infectious diseases and the increase in the drug resistance among microbes has forced the researchers to synthesize biologically active nanoparticles. Improvement of the ecofriendly procedure for the synthesis of nanoparticles is growing day-by-day in the field of nanobiotechnology. In the present study, we use the extract of green alga Botryococcus braunii for the synthesis of copper and silver nanoparticles. The characterization of copper and silver nanoparticles was carried out by using UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron spectroscopy (SEM). FTIR measurements showed all functional groups having control over reduction and stabilization of the nanoparticles. The X-ray diffraction pattern revealed that the particles were crystalline in nature with a face-centred cubic (FCC) geometry. SEM micrographs have shown the morphology of biogenically synthesized metal nanoparticles. Furthermore, these biosynthesized nanoparticles were found to be highly toxic against two Gram-negative bacterial strains Pseudomonas aeruginosa (MTCC 441) and Escherichia coli (MTCC 442), two Gram-positive bacterial strains Klebsiella pneumoniae (MTCC 109) and Staphylococcus aureus (MTCC 96), and a fungal strain Fusarium oxysporum (MTCC 2087). The zone of inhibition was measured by the agar well plate method, and furthermore, minimum inhibitory concentration was determined by the broth dilution assay. |
| format | Article |
| id | doaj-art-264ea63ee19e4017851a5adc35fa67f5 |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-264ea63ee19e4017851a5adc35fa67f52025-02-03T05:43:37ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2018-01-01201810.1155/2018/78794037879403Biogenic Synthesis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial ActivityAnju Arya0Khushbu Gupta1Tejpal Singh Chundawat2Dipti Vaya3Department of Applied Sciences, The North Cap University, Sector 23-A, Gurugram 122017, Haryana, IndiaDepartment of Applied Sciences, The North Cap University, Sector 23-A, Gurugram 122017, Haryana, IndiaDepartment of Applied Sciences, The North Cap University, Sector 23-A, Gurugram 122017, Haryana, IndiaDepartment of Chemistry, Amity University, Gurugram, Haryana, IndiaThe spread of infectious diseases and the increase in the drug resistance among microbes has forced the researchers to synthesize biologically active nanoparticles. Improvement of the ecofriendly procedure for the synthesis of nanoparticles is growing day-by-day in the field of nanobiotechnology. In the present study, we use the extract of green alga Botryococcus braunii for the synthesis of copper and silver nanoparticles. The characterization of copper and silver nanoparticles was carried out by using UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron spectroscopy (SEM). FTIR measurements showed all functional groups having control over reduction and stabilization of the nanoparticles. The X-ray diffraction pattern revealed that the particles were crystalline in nature with a face-centred cubic (FCC) geometry. SEM micrographs have shown the morphology of biogenically synthesized metal nanoparticles. Furthermore, these biosynthesized nanoparticles were found to be highly toxic against two Gram-negative bacterial strains Pseudomonas aeruginosa (MTCC 441) and Escherichia coli (MTCC 442), two Gram-positive bacterial strains Klebsiella pneumoniae (MTCC 109) and Staphylococcus aureus (MTCC 96), and a fungal strain Fusarium oxysporum (MTCC 2087). The zone of inhibition was measured by the agar well plate method, and furthermore, minimum inhibitory concentration was determined by the broth dilution assay.http://dx.doi.org/10.1155/2018/7879403 |
| spellingShingle | Anju Arya Khushbu Gupta Tejpal Singh Chundawat Dipti Vaya Biogenic Synthesis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial Activity Bioinorganic Chemistry and Applications |
| title | Biogenic Synthesis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial Activity |
| title_full | Biogenic Synthesis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial Activity |
| title_fullStr | Biogenic Synthesis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial Activity |
| title_full_unstemmed | Biogenic Synthesis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial Activity |
| title_short | Biogenic Synthesis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial Activity |
| title_sort | biogenic synthesis of copper and silver nanoparticles using green alga botryococcus braunii and its antimicrobial activity |
| url | http://dx.doi.org/10.1155/2018/7879403 |
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