Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp health
This study evaluated the efficacy of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum in improving the immune response and resistance of whiteleg shrimp to prevent Vibrio parahaemolyticus infection. The study consisted of two phases, an in vitro phase to determine the compounds contained i...
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Asosiasi Sains Akuakultur Indonesia
2025-01-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/54134 |
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| author | Mohamad Iqbal Kurniawinata Sukenda Sukenda Dinamella Wahjuningrum Widanarni Widanarni Julie Ekasari |
| author_facet | Mohamad Iqbal Kurniawinata Sukenda Sukenda Dinamella Wahjuningrum Widanarni Widanarni Julie Ekasari |
| author_sort | Mohamad Iqbal Kurniawinata |
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This study evaluated the efficacy of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum in improving the immune response and resistance of whiteleg shrimp to prevent Vibrio parahaemolyticus infection. The study consisted of two phases, an in vitro phase to determine the compounds contained in three medicinal plants as antibacterials, followed by an in vivo phase to evaluate the effect of the medical plant extract on immune response and robustness against V. parahaemolyticus. The results from the first phase revealed that bioactive compounds present in E. longifolia were more varied and had higher concentrations with a lower bactericidal value when compared to those found in C. zedoaria or A. sativum. In the second phase of the experiment, the medicinal plant extract was added to the feed with a dose that was determined according to the first phase results. The treatments tested in the second phase were 1.6% E. longifolia extract dietary addition (EL16), 6.4% C. zedoaria extract dietary addition (CZ64), 6.4% A. sativum extract dietary addition (AS64) and phytobiotics mixture of 1:1:1 (C1) dietary addition, as well as no phytobiotic for negative control treatment and positive control. The results from the second stage demonstrated that dietary phytobiotic extract addition enhances the immunological responses and improves the shrimp survival against V. parahaemolyticus challenge compared to the control group. In conclusion, E. longifolia, C. zedoaria, and A. sativum showed different bioactive compound profiles, which affect their efficacy against V. parahaemolyticus, with EL16 showing higher efficacy.
Keywords: A. sativum, C. zedoaria, E. longifolia, Penaeus vannamei, Phytobiotic
ABSTRAK
Penelitian ini mengevaluasi efikasi Eurycoma longifolia, Curcuma zedoaria, dan Allium sativum dalam meningkatkan respon imun dan resistensi udang vaname untuk mencegah infeksi Vibrio parahaemolyticus. Penelitian ini terdiri dari dua tahap: tahap in vitro untuk menentukan senyawa yang terkandung dalam tiga tanaman obat sebagai antibakteri, diikuti oleh tahap in vivo untuk mengevaluasi efek ekstrak tanaman obat terhadap respon imun dan ketahanan terhadap V. parahaemolyticus. Hasil dari tahap pertama mengungkapkan bahwa senyawa bioaktif yang ada dalam E. longifolia lebih bervariasi dan memiliki konsentrasi yang lebih tinggi dengan nilai bakterisida yang lebih rendah jika dibandingkan dengan yang ditemukan di C. zedoaria atau A. sativum. Pada percobaan tahap kedua, ekstrak tanaman obat ditambahkan ke pakan dengan dosis yang ditentukan sesuai dengan hasil tahap pertama. Perlakuan yang diuji pada tahap kedua adalah penambahan 1,6% ekstrak E. longifolia dalam pakan (EL16), penambahan 6,4% ekstrak C. zedoaria dalam pakan (CZ64), penambahan 6,4% ekstrak A. sativum dalam pakan (AS64) dan campuran fitobiotik 1:1:1 (C1) dalam pakan, serta tanpa fitobiotik untuk perlakuan kontrol negatif dan kontrol positif. Hasil dari tahap kedua menunjukkan bahwa penambahan ekstrak fitobiotik dalam pakan meningkatkan respons imunologi dan memperbaiki kelangsungan hidup udang terhadap tantangan V. parahaemolyticus dibandingkan dengan kelompok kontrol. Sebagai kesimpulan, E. longifolia, C. zedoaria, dan A. sativum menunjukkan profil senyawa bioaktif yang berbeda, yang mempengaruhi efikasinya terhadap V. parahaemolyticus, dengan EL16 menunjukkan efikasi yang lebih tinggi.
Kata kunci: Allium sativum, Curcuma zedoaria, Eurycoma. longifolia, fitobiotik, Penaeus vannamei
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| format | Article |
| id | doaj-art-264b71799f924035b495d10f8838c08e |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-264b71799f924035b495d10f8838c08e2025-01-24T04:32:31ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002025-01-0124110.19027/jai.24.1.71-8154134Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp healthMohamad Iqbal Kurniawinata0Sukenda Sukenda1Dinamella Wahjuningrum2Widanarni Widanarni3Julie Ekasari4Technology and Management of Applied Aquaculutre Study Program, College of Vocational Studies, IPB University, Bogor, West Java, 16128, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java 16680, Indonesia This study evaluated the efficacy of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum in improving the immune response and resistance of whiteleg shrimp to prevent Vibrio parahaemolyticus infection. The study consisted of two phases, an in vitro phase to determine the compounds contained in three medicinal plants as antibacterials, followed by an in vivo phase to evaluate the effect of the medical plant extract on immune response and robustness against V. parahaemolyticus. The results from the first phase revealed that bioactive compounds present in E. longifolia were more varied and had higher concentrations with a lower bactericidal value when compared to those found in C. zedoaria or A. sativum. In the second phase of the experiment, the medicinal plant extract was added to the feed with a dose that was determined according to the first phase results. The treatments tested in the second phase were 1.6% E. longifolia extract dietary addition (EL16), 6.4% C. zedoaria extract dietary addition (CZ64), 6.4% A. sativum extract dietary addition (AS64) and phytobiotics mixture of 1:1:1 (C1) dietary addition, as well as no phytobiotic for negative control treatment and positive control. The results from the second stage demonstrated that dietary phytobiotic extract addition enhances the immunological responses and improves the shrimp survival against V. parahaemolyticus challenge compared to the control group. In conclusion, E. longifolia, C. zedoaria, and A. sativum showed different bioactive compound profiles, which affect their efficacy against V. parahaemolyticus, with EL16 showing higher efficacy. Keywords: A. sativum, C. zedoaria, E. longifolia, Penaeus vannamei, Phytobiotic ABSTRAK Penelitian ini mengevaluasi efikasi Eurycoma longifolia, Curcuma zedoaria, dan Allium sativum dalam meningkatkan respon imun dan resistensi udang vaname untuk mencegah infeksi Vibrio parahaemolyticus. Penelitian ini terdiri dari dua tahap: tahap in vitro untuk menentukan senyawa yang terkandung dalam tiga tanaman obat sebagai antibakteri, diikuti oleh tahap in vivo untuk mengevaluasi efek ekstrak tanaman obat terhadap respon imun dan ketahanan terhadap V. parahaemolyticus. Hasil dari tahap pertama mengungkapkan bahwa senyawa bioaktif yang ada dalam E. longifolia lebih bervariasi dan memiliki konsentrasi yang lebih tinggi dengan nilai bakterisida yang lebih rendah jika dibandingkan dengan yang ditemukan di C. zedoaria atau A. sativum. Pada percobaan tahap kedua, ekstrak tanaman obat ditambahkan ke pakan dengan dosis yang ditentukan sesuai dengan hasil tahap pertama. Perlakuan yang diuji pada tahap kedua adalah penambahan 1,6% ekstrak E. longifolia dalam pakan (EL16), penambahan 6,4% ekstrak C. zedoaria dalam pakan (CZ64), penambahan 6,4% ekstrak A. sativum dalam pakan (AS64) dan campuran fitobiotik 1:1:1 (C1) dalam pakan, serta tanpa fitobiotik untuk perlakuan kontrol negatif dan kontrol positif. Hasil dari tahap kedua menunjukkan bahwa penambahan ekstrak fitobiotik dalam pakan meningkatkan respons imunologi dan memperbaiki kelangsungan hidup udang terhadap tantangan V. parahaemolyticus dibandingkan dengan kelompok kontrol. Sebagai kesimpulan, E. longifolia, C. zedoaria, dan A. sativum menunjukkan profil senyawa bioaktif yang berbeda, yang mempengaruhi efikasinya terhadap V. parahaemolyticus, dengan EL16 menunjukkan efikasi yang lebih tinggi. Kata kunci: Allium sativum, Curcuma zedoaria, Eurycoma. longifolia, fitobiotik, Penaeus vannamei https://journal.ipb.ac.id/index.php/jai/article/view/54134 |
| spellingShingle | Mohamad Iqbal Kurniawinata Sukenda Sukenda Dinamella Wahjuningrum Widanarni Widanarni Julie Ekasari Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp health Jurnal Akuakultur Indonesia |
| title | Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp health |
| title_full | Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp health |
| title_fullStr | Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp health |
| title_full_unstemmed | Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp health |
| title_short | Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp health |
| title_sort | potential of eurycoma longifolia curcuma zedoaria and allium sativum extracts as phytobiotics for shrimp health |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/54134 |
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