Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig Hearts
In cardiovascular surgery ischemia-reperfusion injury is a challenging problem, which needs medical intervention. We investigated the effects of curcumin on cardiac, myocardial, and mitochondrial parameters in perfused isolated working Guinea pig hearts. After preliminary experiments to establish th...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Advances in Pharmacological Sciences |
| Online Access: | http://dx.doi.org/10.1155/2016/6173648 |
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| author | Ermita I. Ibrahim Ilyas Busjra M. Nur Sonny P. Laksono Anton Bahtiar Ari Estuningtyas Caecilia Vitasyana Dede Kusmana Frans D. Suyatna Muhammad Kamil Tadjudin Hans-Joachim Freisleben |
| author_facet | Ermita I. Ibrahim Ilyas Busjra M. Nur Sonny P. Laksono Anton Bahtiar Ari Estuningtyas Caecilia Vitasyana Dede Kusmana Frans D. Suyatna Muhammad Kamil Tadjudin Hans-Joachim Freisleben |
| author_sort | Ermita I. Ibrahim Ilyas |
| collection | DOAJ |
| description | In cardiovascular surgery ischemia-reperfusion injury is a challenging problem, which needs medical intervention. We investigated the effects of curcumin on cardiac, myocardial, and mitochondrial parameters in perfused isolated working Guinea pig hearts. After preliminary experiments to establish the model, normoxia was set at 30 minutes, hypoxia was set at 60, and subsequent reoxygenation was set at 30 minutes. Curcumin was applied in the perfusion buffer at 0.25 and 0.5 μM concentrations. Cardiac parameters measured were afterload, coronary and aortic flows, and systolic and diastolic pressure. In the myocardium histopathology and AST in the perfusate indicated cell damage after hypoxia and malondialdehyde (MDA) levels increased to 232.5% of controls during reoxygenation. Curcumin protected partially against reoxygenation injury without statistically significant differences between the two dosages. Mitochondrial MDA was also increased in reoxygenation (165% of controls), whereas glutathione was diminished (35.2%) as well as glutathione reductase (29.3%), which was significantly increased again to 62.0% by 0.05 μM curcumin. Glutathione peroxidase (GPx) was strongly increased in hypoxia and even more in reoxygenation (255% of controls). Curcumin partly counteracted this increase and attenuated GPx activity independently in hypoxia and in reoxygenation, 0.25 μM concentration to 150% and 0.5 μM concentration to 200% of normoxic activity. |
| format | Article |
| id | doaj-art-26415e3afbd14a2080d751d6a45de6ed |
| institution | Kabale University |
| issn | 1687-6334 1687-6342 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological Sciences |
| spelling | doaj-art-26415e3afbd14a2080d751d6a45de6ed2025-02-03T01:03:05ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422016-01-01201610.1155/2016/61736486173648Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig HeartsErmita I. Ibrahim Ilyas0Busjra M. Nur1Sonny P. Laksono2Anton Bahtiar3Ari Estuningtyas4Caecilia Vitasyana5Dede Kusmana6Frans D. Suyatna7Muhammad Kamil Tadjudin8Hans-Joachim Freisleben9Department of Physiology, Faculty of Medicine, University of Indonesia, Jakarta 10430, IndonesiaDepartment of Physiology, Faculty of Medicine, University of Indonesia, Jakarta 10430, IndonesiaDepartment of Physiology, Faculty of Medicine, University of Indonesia, Jakarta 10430, IndonesiaDepartment of Physiology, Faculty of Medicine, University of Indonesia, Jakarta 10430, IndonesiaDepartment of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta 10430, IndonesiaDepartment of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta 10430, IndonesiaNational Cardiovascular Center, Harapan Kita Hospital and Department of Cardiology and Vascular Medicine, University of Indonesia, Jakarta 10430, IndonesiaDepartment of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta 10430, IndonesiaDepartment of Medical Biology, Faculty of Medicine, University of Indonesia, Jakarta 10430, IndonesiaMedical Research Unit, Faculty of Medicine, University of Indonesia, Jakarta 10430, IndonesiaIn cardiovascular surgery ischemia-reperfusion injury is a challenging problem, which needs medical intervention. We investigated the effects of curcumin on cardiac, myocardial, and mitochondrial parameters in perfused isolated working Guinea pig hearts. After preliminary experiments to establish the model, normoxia was set at 30 minutes, hypoxia was set at 60, and subsequent reoxygenation was set at 30 minutes. Curcumin was applied in the perfusion buffer at 0.25 and 0.5 μM concentrations. Cardiac parameters measured were afterload, coronary and aortic flows, and systolic and diastolic pressure. In the myocardium histopathology and AST in the perfusate indicated cell damage after hypoxia and malondialdehyde (MDA) levels increased to 232.5% of controls during reoxygenation. Curcumin protected partially against reoxygenation injury without statistically significant differences between the two dosages. Mitochondrial MDA was also increased in reoxygenation (165% of controls), whereas glutathione was diminished (35.2%) as well as glutathione reductase (29.3%), which was significantly increased again to 62.0% by 0.05 μM curcumin. Glutathione peroxidase (GPx) was strongly increased in hypoxia and even more in reoxygenation (255% of controls). Curcumin partly counteracted this increase and attenuated GPx activity independently in hypoxia and in reoxygenation, 0.25 μM concentration to 150% and 0.5 μM concentration to 200% of normoxic activity.http://dx.doi.org/10.1155/2016/6173648 |
| spellingShingle | Ermita I. Ibrahim Ilyas Busjra M. Nur Sonny P. Laksono Anton Bahtiar Ari Estuningtyas Caecilia Vitasyana Dede Kusmana Frans D. Suyatna Muhammad Kamil Tadjudin Hans-Joachim Freisleben Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig Hearts Advances in Pharmacological Sciences |
| title | Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig Hearts |
| title_full | Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig Hearts |
| title_fullStr | Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig Hearts |
| title_full_unstemmed | Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig Hearts |
| title_short | Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig Hearts |
| title_sort | effects of curcumin on parameters of myocardial oxidative stress and of mitochondrial glutathione turnover in reoxygenation after 60 minutes of hypoxia in isolated perfused working guinea pig hearts |
| url | http://dx.doi.org/10.1155/2016/6173648 |
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