Effect of Grovth Medium on L-Dopa and Dopamine Production Using Citrobacter freundii (NRRL B-2643)
In this study, microbial production of L-Dopa and Dopamine which is an important substrance for the treatment of Parkinson's disease were investigated by using Citrobacter freundii (NRRL B-2643). The effects of carbon source (sucrose) and, salt concentrations (NaCl, CaCl2), initial pH, temperat...
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Çanakkale Onsekiz Mart University
2022-12-01
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| Series: | Journal of Advanced Research in Natural and Applied Sciences |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/2397873 |
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| author | Sinan Saydam Dursun Özer Veyis Selen Meltem Çakmak Fikret Karataş |
| author_facet | Sinan Saydam Dursun Özer Veyis Selen Meltem Çakmak Fikret Karataş |
| author_sort | Sinan Saydam |
| collection | DOAJ |
| description | In this study, microbial production of L-Dopa and Dopamine which is an important substrance for the treatment of Parkinson's disease were investigated by using Citrobacter freundii (NRRL B-2643). The effects of carbon source (sucrose) and, salt concentrations (NaCl, CaCl2), initial pH, temperature, inoculum level and shaking speed on L-Dopa and dopamine production were investigated. The amounts of extracellular L-dopa and dopamine were determined by using HPLC. Maximum L-dopa and dopamine production, under optimized conditions (sucrose: 2.5 g/L, NaCl and CaCl2: 1.0 g/L, inoculum level: 1.0% (v/v), initial pH: 6.5, temperature: 33°C, shaking speed: 200 rpm) were found to be 458 and 592 mg/L, respectively. Although the experiments were carried out for 60 hours, but the maximum production of L-Dopa and Dopamine was realized at around the 30th hour of the experiments. |
| format | Article |
| id | doaj-art-261672a49e1a49fca5c7e4c586d82abc |
| institution | Kabale University |
| issn | 2757-5195 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Çanakkale Onsekiz Mart University |
| record_format | Article |
| series | Journal of Advanced Research in Natural and Applied Sciences |
| spelling | doaj-art-261672a49e1a49fca5c7e4c586d82abc2025-02-05T17:57:35ZengÇanakkale Onsekiz Mart UniversityJournal of Advanced Research in Natural and Applied Sciences2757-51952022-12-018478579510.28979/jarnas.1109192453Effect of Grovth Medium on L-Dopa and Dopamine Production Using Citrobacter freundii (NRRL B-2643)Sinan Saydam0https://orcid.org/0000-0003-1531-5454Dursun Özer1https://orcid.org/0000-0002-7225-8903Veyis Selen2https://orcid.org/0000-0003-0016-0840Meltem Çakmak3https://orcid.org/0000-0002-6291-863XFikret Karataş4https://orcid.org/0000-0002-0884-027XFIRAT ÜNİVERSİTESİFIRAT ÜNİVERSİTESİFIRAT ÜNİVERSİTESİFIRAT ÜNİVERSİTESİFIRAT ÜNİVERSİTESİ, FEN FAKÜLTESİIn this study, microbial production of L-Dopa and Dopamine which is an important substrance for the treatment of Parkinson's disease were investigated by using Citrobacter freundii (NRRL B-2643). The effects of carbon source (sucrose) and, salt concentrations (NaCl, CaCl2), initial pH, temperature, inoculum level and shaking speed on L-Dopa and dopamine production were investigated. The amounts of extracellular L-dopa and dopamine were determined by using HPLC. Maximum L-dopa and dopamine production, under optimized conditions (sucrose: 2.5 g/L, NaCl and CaCl2: 1.0 g/L, inoculum level: 1.0% (v/v), initial pH: 6.5, temperature: 33°C, shaking speed: 200 rpm) were found to be 458 and 592 mg/L, respectively. Although the experiments were carried out for 60 hours, but the maximum production of L-Dopa and Dopamine was realized at around the 30th hour of the experiments.https://dergipark.org.tr/en/download/article-file/2397873l-dopadopaminefermentationhplccitrobacterfreundii (nrrl b-2643) |
| spellingShingle | Sinan Saydam Dursun Özer Veyis Selen Meltem Çakmak Fikret Karataş Effect of Grovth Medium on L-Dopa and Dopamine Production Using Citrobacter freundii (NRRL B-2643) Journal of Advanced Research in Natural and Applied Sciences l-dopa dopamine fermentation hplc citrobacter freundii (nrrl b-2643) |
| title | Effect of Grovth Medium on L-Dopa and Dopamine Production Using Citrobacter freundii (NRRL B-2643) |
| title_full | Effect of Grovth Medium on L-Dopa and Dopamine Production Using Citrobacter freundii (NRRL B-2643) |
| title_fullStr | Effect of Grovth Medium on L-Dopa and Dopamine Production Using Citrobacter freundii (NRRL B-2643) |
| title_full_unstemmed | Effect of Grovth Medium on L-Dopa and Dopamine Production Using Citrobacter freundii (NRRL B-2643) |
| title_short | Effect of Grovth Medium on L-Dopa and Dopamine Production Using Citrobacter freundii (NRRL B-2643) |
| title_sort | effect of grovth medium on l dopa and dopamine production using citrobacter freundii nrrl b 2643 |
| topic | l-dopa dopamine fermentation hplc citrobacter freundii (nrrl b-2643) |
| url | https://dergipark.org.tr/en/download/article-file/2397873 |
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