Effect of Grovth Medium on L-Dopa and Dopamine Production Using Citrobacter freundii (NRRL B-2643)
In this study, microbial production of L-Dopa and Dopamine which is an important substrance for the treatment of Parkinson's disease were investigated by using Citrobacter freundii (NRRL B-2643). The effects of carbon source (sucrose) and, salt concentrations (NaCl, CaCl2), initial pH, temperat...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Çanakkale Onsekiz Mart University
2022-12-01
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| Series: | Journal of Advanced Research in Natural and Applied Sciences |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/download/article-file/2397873 |
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| Summary: | In this study, microbial production of L-Dopa and Dopamine which is an important substrance for the treatment of Parkinson's disease were investigated by using Citrobacter freundii (NRRL B-2643). The effects of carbon source (sucrose) and, salt concentrations (NaCl, CaCl2), initial pH, temperature, inoculum level and shaking speed on L-Dopa and dopamine production were investigated. The amounts of extracellular L-dopa and dopamine were determined by using HPLC. Maximum L-dopa and dopamine production, under optimized conditions (sucrose: 2.5 g/L, NaCl and CaCl2: 1.0 g/L, inoculum level: 1.0% (v/v), initial pH: 6.5, temperature: 33°C, shaking speed: 200 rpm) were found to be 458 and 592 mg/L, respectively. Although the experiments were carried out for 60 hours, but the maximum production of L-Dopa and Dopamine was realized at around the 30th hour of the experiments. |
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| ISSN: | 2757-5195 |