Biosynthesized ZnO NPs loaded-electrospun PVA/sodium alginate/glycine nanofibers: synthesis, spinning optimization and antimicrobial activity evaluation
Abstract In this work, microalgae-based zinc oxide nanoparticles loaded with electrospun polyvinyl alcohol (PVA)/sodium alginate (SA) nanofibers were fabricated by electro-spinner. PVA/SA fibrous mats were crosslinked by citric acid, which enhanced their thermal stability and swelling behavior. Gree...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-81939-1 |
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| author | El-Refaie Kenawy Elbadawy A. Kamoun Amany Shehata Shahira H. El-Moslamy Adel A.-H. Abdel-Rahman |
| author_facet | El-Refaie Kenawy Elbadawy A. Kamoun Amany Shehata Shahira H. El-Moslamy Adel A.-H. Abdel-Rahman |
| author_sort | El-Refaie Kenawy |
| collection | DOAJ |
| description | Abstract In this work, microalgae-based zinc oxide nanoparticles loaded with electrospun polyvinyl alcohol (PVA)/sodium alginate (SA) nanofibers were fabricated by electro-spinner. PVA/SA fibrous mats were crosslinked by citric acid, which enhanced their thermal stability and swelling behavior. Green-synthesized ZnO NPs were laboratory synthesized and characterized by FTIR, XRD, EDX, SEM, TEM and TGA analyses. Electrospinning of PVA/SA with optimized mixing ratios was further carried out with different ratios of ZnO NPs of (0.25, 0.50 and 0.75%, wt./v) to fabricate SA/PVA/ZnO composite nanofibers. The formula of NF of (SA/PVA/ZnO (0.75%) with different ratios of glycine was used for testing as topical wound dressing as promised biomaterials. Results revealed that NF formula coded AS3.3; composed of (10%PVA/1.5%SA/10%CA/0.75%ZnO/0.5% glycine), in contrast to all the other formulations examined; showed marked reduced percentages of biofilm development of both Gram-positive and Gram-negative bacteria in relation to fungal cells. The relative viable count for Bacillus cereus (98.40 ± 0.51%), Salmonella paratyphi (98.33 ± 0.79%), and Candida albicans (94.29 ± 0.76%) decreased significantly after 36-hour of incubation period with AS3.3 treatment. The obtained findings offered that the prepared composite nanofiber based on ZnO NPs and glycine; could be used as a promising and excellent antimicrobial dressing for inhibiting the microbial growth for the topical wounds healing. |
| format | Article |
| id | doaj-art-2614941b6bdb4e8db7cb03e9aa486bf8 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-2614941b6bdb4e8db7cb03e9aa486bf82025-01-19T12:23:10ZengNature PortfolioScientific Reports2045-23222025-01-0115111910.1038/s41598-024-81939-1Biosynthesized ZnO NPs loaded-electrospun PVA/sodium alginate/glycine nanofibers: synthesis, spinning optimization and antimicrobial activity evaluationEl-Refaie Kenawy0Elbadawy A. Kamoun1Amany Shehata2Shahira H. El-Moslamy3Adel A.-H. Abdel-Rahman4Chemistry Dep, Faculty of Science, Tanta UniversityDepartment of Chemistry, College of Science, King Faisal UniversityChemistry Department, Faculty of Science, Menoufia UniversityBioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City)Chemistry Department, Faculty of Science, Menoufia UniversityAbstract In this work, microalgae-based zinc oxide nanoparticles loaded with electrospun polyvinyl alcohol (PVA)/sodium alginate (SA) nanofibers were fabricated by electro-spinner. PVA/SA fibrous mats were crosslinked by citric acid, which enhanced their thermal stability and swelling behavior. Green-synthesized ZnO NPs were laboratory synthesized and characterized by FTIR, XRD, EDX, SEM, TEM and TGA analyses. Electrospinning of PVA/SA with optimized mixing ratios was further carried out with different ratios of ZnO NPs of (0.25, 0.50 and 0.75%, wt./v) to fabricate SA/PVA/ZnO composite nanofibers. The formula of NF of (SA/PVA/ZnO (0.75%) with different ratios of glycine was used for testing as topical wound dressing as promised biomaterials. Results revealed that NF formula coded AS3.3; composed of (10%PVA/1.5%SA/10%CA/0.75%ZnO/0.5% glycine), in contrast to all the other formulations examined; showed marked reduced percentages of biofilm development of both Gram-positive and Gram-negative bacteria in relation to fungal cells. The relative viable count for Bacillus cereus (98.40 ± 0.51%), Salmonella paratyphi (98.33 ± 0.79%), and Candida albicans (94.29 ± 0.76%) decreased significantly after 36-hour of incubation period with AS3.3 treatment. The obtained findings offered that the prepared composite nanofiber based on ZnO NPs and glycine; could be used as a promising and excellent antimicrobial dressing for inhibiting the microbial growth for the topical wounds healing.https://doi.org/10.1038/s41598-024-81939-1PVA/Sodium AlginateGreen-synthesized ZnO NPsElectrospinningGlycineWound dressing |
| spellingShingle | El-Refaie Kenawy Elbadawy A. Kamoun Amany Shehata Shahira H. El-Moslamy Adel A.-H. Abdel-Rahman Biosynthesized ZnO NPs loaded-electrospun PVA/sodium alginate/glycine nanofibers: synthesis, spinning optimization and antimicrobial activity evaluation Scientific Reports PVA/Sodium Alginate Green-synthesized ZnO NPs Electrospinning Glycine Wound dressing |
| title | Biosynthesized ZnO NPs loaded-electrospun PVA/sodium alginate/glycine nanofibers: synthesis, spinning optimization and antimicrobial activity evaluation |
| title_full | Biosynthesized ZnO NPs loaded-electrospun PVA/sodium alginate/glycine nanofibers: synthesis, spinning optimization and antimicrobial activity evaluation |
| title_fullStr | Biosynthesized ZnO NPs loaded-electrospun PVA/sodium alginate/glycine nanofibers: synthesis, spinning optimization and antimicrobial activity evaluation |
| title_full_unstemmed | Biosynthesized ZnO NPs loaded-electrospun PVA/sodium alginate/glycine nanofibers: synthesis, spinning optimization and antimicrobial activity evaluation |
| title_short | Biosynthesized ZnO NPs loaded-electrospun PVA/sodium alginate/glycine nanofibers: synthesis, spinning optimization and antimicrobial activity evaluation |
| title_sort | biosynthesized zno nps loaded electrospun pva sodium alginate glycine nanofibers synthesis spinning optimization and antimicrobial activity evaluation |
| topic | PVA/Sodium Alginate Green-synthesized ZnO NPs Electrospinning Glycine Wound dressing |
| url | https://doi.org/10.1038/s41598-024-81939-1 |
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