Role of Cathepsin S in Periodontal Inflammation and Infection
Cathepsin S is a cysteine protease and regulator of autophagy with possible involvement in periodontitis. The objective of this study was to investigate whether cathepsin S is involved in the pathogenesis of periodontal diseases. Human periodontal fibroblasts were cultured under inflammatory and inf...
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| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/4786170 |
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| author | S. Memmert A. Damanaki A. V. B. Nogueira S. Eick M. Nokhbehsaim A. K. Papadopoulou A. Till B. Rath S. Jepsen W. Götz C. Piperi E. K. Basdra J. A. Cirelli A. Jäger J. Deschner |
| author_facet | S. Memmert A. Damanaki A. V. B. Nogueira S. Eick M. Nokhbehsaim A. K. Papadopoulou A. Till B. Rath S. Jepsen W. Götz C. Piperi E. K. Basdra J. A. Cirelli A. Jäger J. Deschner |
| author_sort | S. Memmert |
| collection | DOAJ |
| description | Cathepsin S is a cysteine protease and regulator of autophagy with possible involvement in periodontitis. The objective of this study was to investigate whether cathepsin S is involved in the pathogenesis of periodontal diseases. Human periodontal fibroblasts were cultured under inflammatory and infectious conditions elicited by interleukin-1β and Fusobacterium nucleatum, respectively. An array-based approach was used to analyze differential expression of autophagy-associated genes. Cathepsin S was upregulated most strongly and thus further studied in vitro at gene and protein levels. In vivo, gingival tissue biopsies from rats with ligature-induced periodontitis and from periodontitis patients were also analyzed at transcriptional and protein levels. Multiple gene expression changes due to interleukin-1β and F. nucleatum were observed in vitro. Both stimulants caused a significant cathepsin S upregulation. A significantly elevated cathepsin S expression in gingival biopsies from rats with experimental periodontitis was found in vivo, as compared to that from control. Gingival biopsies from periodontitis patients showed a significantly higher cathepsin S expression than those from healthy gingiva. Our findings provide original evidence that cathepsin S is increased in periodontal cells and tissues under inflammatory and infectious conditions, suggesting a critical role of this autophagy-associated molecule in the pathogenesis of periodontitis. |
| format | Article |
| id | doaj-art-25f310afe6034165a67654f127794968 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-25f310afe6034165a67654f1277949682025-02-03T01:28:48ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/47861704786170Role of Cathepsin S in Periodontal Inflammation and InfectionS. Memmert0A. Damanaki1A. V. B. Nogueira2S. Eick3M. Nokhbehsaim4A. K. Papadopoulou5A. Till6B. Rath7S. Jepsen8W. Götz9C. Piperi10E. K. Basdra11J. A. Cirelli12A. Jäger13J. Deschner14Section of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanySection of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanyDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University (UNESP), Araraquara, SP, BrazilDepartment of Periodontology, Laboratory for Oral Microbiology, School of Dental Medicine, University of Bern, Bern, SwitzerlandSection of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanyDiscipline of Orthodontics, Faculty of Dentistry, University of Sydney, Sydney, NSW, AustraliaInstitute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn, Bonn, GermanyDepartment of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanyDepartment of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, GermanyDepartment of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanyDepartment of Biological Chemistry, Medical School, National and Kapodistrian University of Athens, Athens, GreeceDepartment of Biological Chemistry, Medical School, National and Kapodistrian University of Athens, Athens, GreeceDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University (UNESP), Araraquara, SP, BrazilDepartment of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanySection of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanyCathepsin S is a cysteine protease and regulator of autophagy with possible involvement in periodontitis. The objective of this study was to investigate whether cathepsin S is involved in the pathogenesis of periodontal diseases. Human periodontal fibroblasts were cultured under inflammatory and infectious conditions elicited by interleukin-1β and Fusobacterium nucleatum, respectively. An array-based approach was used to analyze differential expression of autophagy-associated genes. Cathepsin S was upregulated most strongly and thus further studied in vitro at gene and protein levels. In vivo, gingival tissue biopsies from rats with ligature-induced periodontitis and from periodontitis patients were also analyzed at transcriptional and protein levels. Multiple gene expression changes due to interleukin-1β and F. nucleatum were observed in vitro. Both stimulants caused a significant cathepsin S upregulation. A significantly elevated cathepsin S expression in gingival biopsies from rats with experimental periodontitis was found in vivo, as compared to that from control. Gingival biopsies from periodontitis patients showed a significantly higher cathepsin S expression than those from healthy gingiva. Our findings provide original evidence that cathepsin S is increased in periodontal cells and tissues under inflammatory and infectious conditions, suggesting a critical role of this autophagy-associated molecule in the pathogenesis of periodontitis.http://dx.doi.org/10.1155/2017/4786170 |
| spellingShingle | S. Memmert A. Damanaki A. V. B. Nogueira S. Eick M. Nokhbehsaim A. K. Papadopoulou A. Till B. Rath S. Jepsen W. Götz C. Piperi E. K. Basdra J. A. Cirelli A. Jäger J. Deschner Role of Cathepsin S in Periodontal Inflammation and Infection Mediators of Inflammation |
| title | Role of Cathepsin S in Periodontal Inflammation and Infection |
| title_full | Role of Cathepsin S in Periodontal Inflammation and Infection |
| title_fullStr | Role of Cathepsin S in Periodontal Inflammation and Infection |
| title_full_unstemmed | Role of Cathepsin S in Periodontal Inflammation and Infection |
| title_short | Role of Cathepsin S in Periodontal Inflammation and Infection |
| title_sort | role of cathepsin s in periodontal inflammation and infection |
| url | http://dx.doi.org/10.1155/2017/4786170 |
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