Adaptive Immunity in Ankylosing Spondylitis: Phenotype and Functional Alterations of T-Cells before and during Infliximab Therapy
Our aim was to assess the phenotype of T-cell subsets in patients with ankylosing spondylitis (AS), a chronic inflammatory rheumatic disease. In addition, we also tested short-term T-cell activation characteristics. Measurements were done in 13 AS patients before and during the intravenous therapy w...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/808724 |
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| author | Balázs Szalay Gergő Mészáros Áron Cseh Lilla Ács Magdolna Deák László Kovács Barna Vásárhelyi Attila Balog |
| author_facet | Balázs Szalay Gergő Mészáros Áron Cseh Lilla Ács Magdolna Deák László Kovács Barna Vásárhelyi Attila Balog |
| author_sort | Balázs Szalay |
| collection | DOAJ |
| description | Our aim was to assess the phenotype of T-cell subsets in patients with ankylosing spondylitis (AS), a chronic inflammatory rheumatic disease. In addition, we also tested short-term T-cell activation characteristics. Measurements were done in 13 AS patients before and during the intravenous therapy with anti-TNF agent infliximab (IFX).
Flow cytometry was used to determine T-cell subsets in peripheral blood and their intracellular signaling during activation. The prevalence of Th2 and Th17 cells responsible for the regulation of adaptive immunity was higher in AS than in 9 healthy controls. Although IFX therapy improved patients' condition, immune phenotype did not normalize. Cytoplasmic and mitochondrial calcium responses of CD4+ and CD8+ cells to a specific activation were delayed, while NO generation was increased in AS. NO generation normalized sooner upon IFX than calcium response. These results suggest an abnormal immune phenotype with functional disturbances of CD4+ and CD8+ cells in AS. |
| format | Article |
| id | doaj-art-25c572e5b8f6464b963fe1800d35e976 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-25c572e5b8f6464b963fe1800d35e9762025-02-03T01:11:38ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/808724808724Adaptive Immunity in Ankylosing Spondylitis: Phenotype and Functional Alterations of T-Cells before and during Infliximab TherapyBalázs Szalay0Gergő Mészáros1Áron Cseh2Lilla Ács3Magdolna Deák4László Kovács5Barna Vásárhelyi6Attila Balog7First Department of Pediatrics, Semmelweis University, Bókay János Utca 53-54, 1083 Budapest, HungaryFirst Department of Pediatrics, Semmelweis University, Bókay János Utca 53-54, 1083 Budapest, HungaryFirst Department of Pediatrics, Semmelweis University, Bókay János Utca 53-54, 1083 Budapest, HungaryAlbert Szent-Györgyi Health Center, Department of Rheumatology, University of Szeged, Kálvária sgt. 57, 6725, Szeged, HungaryAlbert Szent-Györgyi Health Center, Department of Rheumatology, University of Szeged, Kálvária sgt. 57, 6725, Szeged, HungaryAlbert Szent-Györgyi Health Center, Department of Rheumatology, University of Szeged, Kálvária sgt. 57, 6725, Szeged, HungaryResearch Group of Pediatrics and Nephrology, Hungarian Academy of Sciences, Bókay János Utca 53-54, 1083 Budapest, HungaryAlbert Szent-Györgyi Health Center, Department of Rheumatology, University of Szeged, Kálvária sgt. 57, 6725, Szeged, HungaryOur aim was to assess the phenotype of T-cell subsets in patients with ankylosing spondylitis (AS), a chronic inflammatory rheumatic disease. In addition, we also tested short-term T-cell activation characteristics. Measurements were done in 13 AS patients before and during the intravenous therapy with anti-TNF agent infliximab (IFX). Flow cytometry was used to determine T-cell subsets in peripheral blood and their intracellular signaling during activation. The prevalence of Th2 and Th17 cells responsible for the regulation of adaptive immunity was higher in AS than in 9 healthy controls. Although IFX therapy improved patients' condition, immune phenotype did not normalize. Cytoplasmic and mitochondrial calcium responses of CD4+ and CD8+ cells to a specific activation were delayed, while NO generation was increased in AS. NO generation normalized sooner upon IFX than calcium response. These results suggest an abnormal immune phenotype with functional disturbances of CD4+ and CD8+ cells in AS.http://dx.doi.org/10.1155/2012/808724 |
| spellingShingle | Balázs Szalay Gergő Mészáros Áron Cseh Lilla Ács Magdolna Deák László Kovács Barna Vásárhelyi Attila Balog Adaptive Immunity in Ankylosing Spondylitis: Phenotype and Functional Alterations of T-Cells before and during Infliximab Therapy Clinical and Developmental Immunology |
| title | Adaptive Immunity in Ankylosing Spondylitis: Phenotype and Functional Alterations of T-Cells before and during Infliximab Therapy |
| title_full | Adaptive Immunity in Ankylosing Spondylitis: Phenotype and Functional Alterations of T-Cells before and during Infliximab Therapy |
| title_fullStr | Adaptive Immunity in Ankylosing Spondylitis: Phenotype and Functional Alterations of T-Cells before and during Infliximab Therapy |
| title_full_unstemmed | Adaptive Immunity in Ankylosing Spondylitis: Phenotype and Functional Alterations of T-Cells before and during Infliximab Therapy |
| title_short | Adaptive Immunity in Ankylosing Spondylitis: Phenotype and Functional Alterations of T-Cells before and during Infliximab Therapy |
| title_sort | adaptive immunity in ankylosing spondylitis phenotype and functional alterations of t cells before and during infliximab therapy |
| url | http://dx.doi.org/10.1155/2012/808724 |
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