Neuroprotective Effect of Taurine against Cell Death, Glial Changes, and Neuronal Loss in the Cerebellum of Rats Exposed to Chronic-Recurrent Neuroinflammation Induced by LPS
The present study investigated the neuroprotective effect of taurine against the deleterious effects of chronic-recurrent neuroinflammation induced by LPS in the cerebellum of rats. Adult male Wistar rats were treated with taurine for 28 days. Taurine was administered at a dose of 30 or 100 mg/kg, b...
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/7497185 |
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| author | Samara P. Silva Adriana M. Zago Fabiano B. Carvalho Lucas Germann Gabriela de M. Colombo Francine L. Rahmeier Jessié M. Gutierres Cristina R. Reschke Margarete D. Bagatini Charles E. Assmann Marilda da C. Fernandes |
| author_facet | Samara P. Silva Adriana M. Zago Fabiano B. Carvalho Lucas Germann Gabriela de M. Colombo Francine L. Rahmeier Jessié M. Gutierres Cristina R. Reschke Margarete D. Bagatini Charles E. Assmann Marilda da C. Fernandes |
| author_sort | Samara P. Silva |
| collection | DOAJ |
| description | The present study investigated the neuroprotective effect of taurine against the deleterious effects of chronic-recurrent neuroinflammation induced by LPS in the cerebellum of rats. Adult male Wistar rats were treated with taurine for 28 days. Taurine was administered at a dose of 30 or 100 mg/kg, by gavage. On days 7, 14, 21, and 28, the animals received LPS (250 μg/kg) intraperitoneally. The vehicle used was saline. The animals were divided into six groups: vehicle, taurine 30 mg/kg, taurine 100 mg/kg, LPS, LPS plus taurine 30 mg/kg, and LPS plus taurine 100 mg/kg. On day 29, the animals were euthanized, and the cerebellum was removed and prepared for immunofluorescence analysis using antibodies of GFAP, NeuN, CD11b, and cleaved caspase-3. LPS group showed a reduction in the immunoreactivity of GFAP in the arbor vitae and medullary center and of NeuN in the granular layer of the cerebellar cortex. LPS increased the immunoreactivity of CD11b in the arbor vitae and in the medullary center. Taurine protected against these effects induced by LPS in immunoreactivity of GFAP, NeuN, and CD11b, with the 100 mg/kg dose being the most effective. LPS induced an increase in the number of positive cleaved caspase-3 cells in the Purkinje cell layers, granular layer, arbor vitae, and medullary center. Taurine showed its antiapoptotic activity by reducing the cleaved caspase-3 cells in relation to the LPS group. Here, a potential neuroprotective role of taurine can be seen since this amino acid was effective in protecting the cerebellum of rats against cell death and changes in glial and neuronal cells in the face of chronic-recurrent neuroinflammation. |
| format | Article |
| id | doaj-art-25bfee40502d45b9857d8eca760a5c5f |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | Journal of Immunology Research |
| spelling | doaj-art-25bfee40502d45b9857d8eca760a5c5f2025-02-03T05:43:46ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/74971857497185Neuroprotective Effect of Taurine against Cell Death, Glial Changes, and Neuronal Loss in the Cerebellum of Rats Exposed to Chronic-Recurrent Neuroinflammation Induced by LPSSamara P. Silva0Adriana M. Zago1Fabiano B. Carvalho2Lucas Germann3Gabriela de M. Colombo4Francine L. Rahmeier5Jessié M. Gutierres6Cristina R. Reschke7Margarete D. Bagatini8Charles E. Assmann9Marilda da C. Fernandes10Pathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, BrazilPathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, BrazilPathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, BrazilPathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, BrazilPathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, BrazilPathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, BrazilPathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, BrazilSchool of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, IrelandGraduate Program in Medical Sciences, Federal University of Fronteira Sul, Chapecó, SC, BrazilPostgraduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, BrazilPathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, BrazilThe present study investigated the neuroprotective effect of taurine against the deleterious effects of chronic-recurrent neuroinflammation induced by LPS in the cerebellum of rats. Adult male Wistar rats were treated with taurine for 28 days. Taurine was administered at a dose of 30 or 100 mg/kg, by gavage. On days 7, 14, 21, and 28, the animals received LPS (250 μg/kg) intraperitoneally. The vehicle used was saline. The animals were divided into six groups: vehicle, taurine 30 mg/kg, taurine 100 mg/kg, LPS, LPS plus taurine 30 mg/kg, and LPS plus taurine 100 mg/kg. On day 29, the animals were euthanized, and the cerebellum was removed and prepared for immunofluorescence analysis using antibodies of GFAP, NeuN, CD11b, and cleaved caspase-3. LPS group showed a reduction in the immunoreactivity of GFAP in the arbor vitae and medullary center and of NeuN in the granular layer of the cerebellar cortex. LPS increased the immunoreactivity of CD11b in the arbor vitae and in the medullary center. Taurine protected against these effects induced by LPS in immunoreactivity of GFAP, NeuN, and CD11b, with the 100 mg/kg dose being the most effective. LPS induced an increase in the number of positive cleaved caspase-3 cells in the Purkinje cell layers, granular layer, arbor vitae, and medullary center. Taurine showed its antiapoptotic activity by reducing the cleaved caspase-3 cells in relation to the LPS group. Here, a potential neuroprotective role of taurine can be seen since this amino acid was effective in protecting the cerebellum of rats against cell death and changes in glial and neuronal cells in the face of chronic-recurrent neuroinflammation.http://dx.doi.org/10.1155/2021/7497185 |
| spellingShingle | Samara P. Silva Adriana M. Zago Fabiano B. Carvalho Lucas Germann Gabriela de M. Colombo Francine L. Rahmeier Jessié M. Gutierres Cristina R. Reschke Margarete D. Bagatini Charles E. Assmann Marilda da C. Fernandes Neuroprotective Effect of Taurine against Cell Death, Glial Changes, and Neuronal Loss in the Cerebellum of Rats Exposed to Chronic-Recurrent Neuroinflammation Induced by LPS Journal of Immunology Research |
| title | Neuroprotective Effect of Taurine against Cell Death, Glial Changes, and Neuronal Loss in the Cerebellum of Rats Exposed to Chronic-Recurrent Neuroinflammation Induced by LPS |
| title_full | Neuroprotective Effect of Taurine against Cell Death, Glial Changes, and Neuronal Loss in the Cerebellum of Rats Exposed to Chronic-Recurrent Neuroinflammation Induced by LPS |
| title_fullStr | Neuroprotective Effect of Taurine against Cell Death, Glial Changes, and Neuronal Loss in the Cerebellum of Rats Exposed to Chronic-Recurrent Neuroinflammation Induced by LPS |
| title_full_unstemmed | Neuroprotective Effect of Taurine against Cell Death, Glial Changes, and Neuronal Loss in the Cerebellum of Rats Exposed to Chronic-Recurrent Neuroinflammation Induced by LPS |
| title_short | Neuroprotective Effect of Taurine against Cell Death, Glial Changes, and Neuronal Loss in the Cerebellum of Rats Exposed to Chronic-Recurrent Neuroinflammation Induced by LPS |
| title_sort | neuroprotective effect of taurine against cell death glial changes and neuronal loss in the cerebellum of rats exposed to chronic recurrent neuroinflammation induced by lps |
| url | http://dx.doi.org/10.1155/2021/7497185 |
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