Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa Stems
The study aims to determine the hepatoprotective effect of n-hexane, ethyl acetate, and methanol extracts of the leaves and stems of two Cleome species against carbon tetrachloride- (CCl4-) induced liver toxicity both in vitro using human hepatoma (HepG2) cells and in vivo in rats as well as the hep...
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Wiley
2021-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/5578667 |
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| author | Nhat Minh Phan Thi Hong Tuoi Do Le Thanh Tuyen Nguyen Trong Tuan Nguyen Quoc Luan Ngo Trong Duc Tran Quan Hien Nguyen Bui Linh Chi Huynh Diep Xuan Ky Nguyen Trong Dat Bui Dinh Tri Mai Tan Phat Nguyen |
| author_facet | Nhat Minh Phan Thi Hong Tuoi Do Le Thanh Tuyen Nguyen Trong Tuan Nguyen Quoc Luan Ngo Trong Duc Tran Quan Hien Nguyen Bui Linh Chi Huynh Diep Xuan Ky Nguyen Trong Dat Bui Dinh Tri Mai Tan Phat Nguyen |
| author_sort | Nhat Minh Phan |
| collection | DOAJ |
| description | The study aims to determine the hepatoprotective effect of n-hexane, ethyl acetate, and methanol extracts of the leaves and stems of two Cleome species against carbon tetrachloride- (CCl4-) induced liver toxicity both in vitro using human hepatoma (HepG2) cells and in vivo in rats as well as the hepatoprotective property of all isolated compounds on HepG2. After 72 h of treatment, at the concentrations of 25, 50, and 100 μg/mL, the methanol of C. chelidonii stems (CCSM) ranged from 18.6% to 20.8%, whereas the methanol of C. chelidonii stems (CVSM) increased from 12.3% to 17.2% cell viability. The results show that CCSM and CVSM significantly expressed in vitro hepaprotective activity on HepG2. Therefore, the animals were daily treated with these extracts at the doses of 15, 30, and 45 mg/kg body weight for 5 days, and CCl4 was injected (2 ml/kg body weight, i.p.) on the 2nd and 3rd days. Levels of aspartate aminotransferase (ALT) and alanine aminotransferase (AST) in the blood were measured and compared to the silymarin control. The treatments with CCSM and CVSM (30, and 45 mg/kg) possessed significant hepatoprotection and were comparable with the activity of silymarin. Further, phytochemical studies of these ones were conducted and led to the identification of eight flavonoids: visconoside A (1), visconoside B (2), quercetin 3-O-β-D-glucopyranoside 7-O-α-L-rhamnopyranoside (3), kaempferol 3-O-β-D-glucopyranoside 7-O-α-L-rhamnopyranoside (4), cleomeside A (5), cleomeside B (6), cleomeside C (7), and quercetin-3-O-[β-D-glucopyranosyl-(1⟶2)]-α-L-rhamnopyranoside 7-O-α-L-rhamnopyranoside (8). Two major flavonoids (1 and 4) displayed significant hepatoprotective property (at the concentration of 100 μM, the prevention percentage values were 66.5% and 74.2%, respectively, compared to the quercetin control, with value of 80.3%). |
| format | Article |
| id | doaj-art-2598daef107c42708eb9ed480c02ffc7 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | Journal of Chemistry |
| spelling | doaj-art-2598daef107c42708eb9ed480c02ffc72025-02-03T00:58:51ZengWileyJournal of Chemistry2090-90632090-90712021-01-01202110.1155/2021/55786675578667Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa StemsNhat Minh Phan0Thi Hong Tuoi Do1Le Thanh Tuyen Nguyen2Trong Tuan Nguyen3Quoc Luan Ngo4Trong Duc Tran5Quan Hien Nguyen6Bui Linh Chi Huynh7Diep Xuan Ky Nguyen8Trong Dat Bui9Dinh Tri Mai10Tan Phat Nguyen11Faculty of Chemistry, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Cau Giay, Hanoi, VietnamFaculty of Pharmacy, University of Medicine and Pharmacy, 41 Dinh Tien Hoang District 1, Ho Chi Minh City, VietnamSaigon Pharmaceutical Sciences & Technologies Center, University of Medicine and Pharmacy, 41 Dinh Tien Hoang District 1, Ho Chi Minh City, VietnamCan Tho University, 3/2 Street Ninh Kieu District, Can Tho City, VietnamCan Tho University, 3/2 Street Ninh Kieu District, Can Tho City, VietnamUniversity of the Sunshine Coast, 90 Sippy Downs Drive, Sippy Downs 4556, Queensland, AustraliaHo Chi Minh City Institute of Physics, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi District 1, Ho Chi Minh City, VietnamDepartment of Science, Dong Nai University, Bien Hoa City, Dong Nai, VietnamNatural Products Laboratory, Institute of Chemical Technology, Vietnam Academy of Science and Technology, 01A Thanh Loc 29 Thanh Loc District 12, Ho Chi Minh City, VietnamNatural Products Laboratory, Institute of Chemical Technology, Vietnam Academy of Science and Technology, 01A Thanh Loc 29 Thanh Loc District 12, Ho Chi Minh City, VietnamFaculty of Chemistry, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Cau Giay, Hanoi, VietnamFaculty of Chemistry, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Cau Giay, Hanoi, VietnamThe study aims to determine the hepatoprotective effect of n-hexane, ethyl acetate, and methanol extracts of the leaves and stems of two Cleome species against carbon tetrachloride- (CCl4-) induced liver toxicity both in vitro using human hepatoma (HepG2) cells and in vivo in rats as well as the hepatoprotective property of all isolated compounds on HepG2. After 72 h of treatment, at the concentrations of 25, 50, and 100 μg/mL, the methanol of C. chelidonii stems (CCSM) ranged from 18.6% to 20.8%, whereas the methanol of C. chelidonii stems (CVSM) increased from 12.3% to 17.2% cell viability. The results show that CCSM and CVSM significantly expressed in vitro hepaprotective activity on HepG2. Therefore, the animals were daily treated with these extracts at the doses of 15, 30, and 45 mg/kg body weight for 5 days, and CCl4 was injected (2 ml/kg body weight, i.p.) on the 2nd and 3rd days. Levels of aspartate aminotransferase (ALT) and alanine aminotransferase (AST) in the blood were measured and compared to the silymarin control. The treatments with CCSM and CVSM (30, and 45 mg/kg) possessed significant hepatoprotection and were comparable with the activity of silymarin. Further, phytochemical studies of these ones were conducted and led to the identification of eight flavonoids: visconoside A (1), visconoside B (2), quercetin 3-O-β-D-glucopyranoside 7-O-α-L-rhamnopyranoside (3), kaempferol 3-O-β-D-glucopyranoside 7-O-α-L-rhamnopyranoside (4), cleomeside A (5), cleomeside B (6), cleomeside C (7), and quercetin-3-O-[β-D-glucopyranosyl-(1⟶2)]-α-L-rhamnopyranoside 7-O-α-L-rhamnopyranoside (8). Two major flavonoids (1 and 4) displayed significant hepatoprotective property (at the concentration of 100 μM, the prevention percentage values were 66.5% and 74.2%, respectively, compared to the quercetin control, with value of 80.3%).http://dx.doi.org/10.1155/2021/5578667 |
| spellingShingle | Nhat Minh Phan Thi Hong Tuoi Do Le Thanh Tuyen Nguyen Trong Tuan Nguyen Quoc Luan Ngo Trong Duc Tran Quan Hien Nguyen Bui Linh Chi Huynh Diep Xuan Ky Nguyen Trong Dat Bui Dinh Tri Mai Tan Phat Nguyen Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa Stems Journal of Chemistry |
| title | Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa Stems |
| title_full | Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa Stems |
| title_fullStr | Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa Stems |
| title_full_unstemmed | Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa Stems |
| title_short | Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa Stems |
| title_sort | hepatoprotection and phytochemistry of the vietnamese herbs cleome chelidonii and cleome viscosa stems |
| url | http://dx.doi.org/10.1155/2021/5578667 |
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