Phenotypic detection of carbapenem-resistant Enterobacterales in clinical isolates at a tertiary care hospital
Background: In the past decade, there has been a global emergence of carbapenem-resistant Gram-negative bacilli, especially Enterobacterales. Carbapenem resistance is attributed to the ability of the bacteria to produce carbapenemases. The aim of the study is to detect carbapenem-resistant Enterobac...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-01-01
|
| Series: | Journal of Current Research in Scientific Medicine |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jcrsm.jcrsm_113_23 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832590939299774464 |
|---|---|
| author | Astha Sinha Mamta Gour Riti Jain Seth |
| author_facet | Astha Sinha Mamta Gour Riti Jain Seth |
| author_sort | Astha Sinha |
| collection | DOAJ |
| description | Background:
In the past decade, there has been a global emergence of carbapenem-resistant Gram-negative bacilli, especially Enterobacterales. Carbapenem resistance is attributed to the ability of the bacteria to produce carbapenemases. The aim of the study is to detect carbapenem-resistant Enterobacterales (CRE) in different clinical isolates and study carbapenemase production by phenotypic methods in CRE.
Materials and Methods:
A total of 379 Enterobacterales were isolated from different clinical samples from patients attending outpatient departments and admitted in wards and intensive care units (ICUs). They were tested for carbapenem resistance by Kirby–Bauer disk diffusion method and then tested for carbapenemase production by ethylenediaminetetraacetic acid (EDTA)–disk synergy test and Modified Carbapenem Inactivation Method (mCIM).
Results:
This study was conducted from February 2021 to August 2022. Out of 379 Enterobacterales, 70 (18.47%) were CRE, out of which maximum carbapenem resistance of 23.53% was shown by Klebsiella pneumoniae isolates. The maximum carbapenem resistance was seen in the age group of 16–45 years and the most number of CRE isolates were from ICUs. Phenotypic test results indicated that 54.28% (38/70) of isolates were positive for carbapenemase production by either of the phenotypic methods.
Conclusion:
About one-fifth of the Enterobacterales isolates were carbapenem resistant. This study highlights the use of phenotypic methods to detect carbapenemase production in CRE, which is responsible for multidrug resistance. This information is relevant for surveillance, to implement infection prevention and control practices and antibiotic policies. |
| format | Article |
| id | doaj-art-25710823d77d4e719670a0202727a3c6 |
| institution | Kabale University |
| issn | 2542-6273 2455-3069 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Current Research in Scientific Medicine |
| spelling | doaj-art-25710823d77d4e719670a0202727a3c62025-01-23T05:25:56ZengWolters Kluwer Medknow PublicationsJournal of Current Research in Scientific Medicine2542-62732455-30692024-01-01101747810.4103/jcrsm.jcrsm_113_23Phenotypic detection of carbapenem-resistant Enterobacterales in clinical isolates at a tertiary care hospitalAstha SinhaMamta GourRiti Jain SethBackground: In the past decade, there has been a global emergence of carbapenem-resistant Gram-negative bacilli, especially Enterobacterales. Carbapenem resistance is attributed to the ability of the bacteria to produce carbapenemases. The aim of the study is to detect carbapenem-resistant Enterobacterales (CRE) in different clinical isolates and study carbapenemase production by phenotypic methods in CRE. Materials and Methods: A total of 379 Enterobacterales were isolated from different clinical samples from patients attending outpatient departments and admitted in wards and intensive care units (ICUs). They were tested for carbapenem resistance by Kirby–Bauer disk diffusion method and then tested for carbapenemase production by ethylenediaminetetraacetic acid (EDTA)–disk synergy test and Modified Carbapenem Inactivation Method (mCIM). Results: This study was conducted from February 2021 to August 2022. Out of 379 Enterobacterales, 70 (18.47%) were CRE, out of which maximum carbapenem resistance of 23.53% was shown by Klebsiella pneumoniae isolates. The maximum carbapenem resistance was seen in the age group of 16–45 years and the most number of CRE isolates were from ICUs. Phenotypic test results indicated that 54.28% (38/70) of isolates were positive for carbapenemase production by either of the phenotypic methods. Conclusion: About one-fifth of the Enterobacterales isolates were carbapenem resistant. This study highlights the use of phenotypic methods to detect carbapenemase production in CRE, which is responsible for multidrug resistance. This information is relevant for surveillance, to implement infection prevention and control practices and antibiotic policies.https://journals.lww.com/10.4103/jcrsm.jcrsm_113_23carbapenemase productioncarbapenem-resistant enterobacteralesethylenediaminetetraacetic acid–disk synergy testmodified carbapenem inactivation methodmultidrug resistance |
| spellingShingle | Astha Sinha Mamta Gour Riti Jain Seth Phenotypic detection of carbapenem-resistant Enterobacterales in clinical isolates at a tertiary care hospital Journal of Current Research in Scientific Medicine carbapenemase production carbapenem-resistant enterobacterales ethylenediaminetetraacetic acid–disk synergy test modified carbapenem inactivation method multidrug resistance |
| title | Phenotypic detection of carbapenem-resistant Enterobacterales in clinical isolates at a tertiary care hospital |
| title_full | Phenotypic detection of carbapenem-resistant Enterobacterales in clinical isolates at a tertiary care hospital |
| title_fullStr | Phenotypic detection of carbapenem-resistant Enterobacterales in clinical isolates at a tertiary care hospital |
| title_full_unstemmed | Phenotypic detection of carbapenem-resistant Enterobacterales in clinical isolates at a tertiary care hospital |
| title_short | Phenotypic detection of carbapenem-resistant Enterobacterales in clinical isolates at a tertiary care hospital |
| title_sort | phenotypic detection of carbapenem resistant enterobacterales in clinical isolates at a tertiary care hospital |
| topic | carbapenemase production carbapenem-resistant enterobacterales ethylenediaminetetraacetic acid–disk synergy test modified carbapenem inactivation method multidrug resistance |
| url | https://journals.lww.com/10.4103/jcrsm.jcrsm_113_23 |
| work_keys_str_mv | AT asthasinha phenotypicdetectionofcarbapenemresistantenterobacteralesinclinicalisolatesatatertiarycarehospital AT mamtagour phenotypicdetectionofcarbapenemresistantenterobacteralesinclinicalisolatesatatertiarycarehospital AT ritijainseth phenotypicdetectionofcarbapenemresistantenterobacteralesinclinicalisolatesatatertiarycarehospital |