Nanocomposite of electrochemically reduced graphene oxide and gold nanourchins for electrochemical DNA detection
Abstract A nanocomposite of graphene oxide and gold nanourchins has been used here to modify the surface of a screen‐printed carbon electrode to enhance the sensitivity of the electrochemical DNA detection system. A specific single‐stranded DNA probe was designed based on the target DNA sequence and...
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12086 |
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| author | Mostafa Azimzadeh Zahra Aghili Behrooz Jannat Saeid Jafari Saeed Rafizadeh Tafti Navid Nasirizadeh |
| author_facet | Mostafa Azimzadeh Zahra Aghili Behrooz Jannat Saeid Jafari Saeed Rafizadeh Tafti Navid Nasirizadeh |
| author_sort | Mostafa Azimzadeh |
| collection | DOAJ |
| description | Abstract A nanocomposite of graphene oxide and gold nanourchins has been used here to modify the surface of a screen‐printed carbon electrode to enhance the sensitivity of the electrochemical DNA detection system. A specific single‐stranded DNA probe was designed based on the target DNA sequence and was thiolated to be self‐assembled on the surface of the gold nanourchins placed on the modified electrode. Doxorubicin was used as an electrochemical label to detect the DNA hybridisation using differential pulse voltammetry (DPV). The assembling process was confirmed using scanning electron microscopy (SEM) imaging, cyclic voltammetry (CV), and the EIS method. The high sensitivity of the proposed system led to a low detection limit of 0.16 fM and a wide linear range from 0.5 to 950.0 fM. The specificity of the DNA hybridisation and the signalling molecule (haematoxylin) caused very high selectivity towards the target DNA than other non‐specific sequences. |
| format | Article |
| id | doaj-art-256ac2910bfd4844938e2c2ee86d91ba |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-256ac2910bfd4844938e2c2ee86d91ba2025-02-03T01:29:37ZengWileyIET Nanobiotechnology1751-87411751-875X2022-07-0116519019810.1049/nbt2.12086Nanocomposite of electrochemically reduced graphene oxide and gold nanourchins for electrochemical DNA detectionMostafa Azimzadeh0Zahra Aghili1Behrooz Jannat2Saeid Jafari3Saeed Rafizadeh Tafti4Navid Nasirizadeh5Halal Research Center of IRI, MOH Tehran IranFood & Drug Control Reference Laboratories Center, FDA, MOH Tehran IranHalal Research Center of IRI, MOH Tehran IranDepartment of Textile and Polymer Engineering, Yazd Branch Islamic Azad University Yazd IranMedical Nanotechnology & Tissue Engineering Research Center Yazd Reproductive Sciences Institute Shahid Sadoughi University of Medical Sciences Yazd IranHalal Research Center of IRI, MOH Tehran IranAbstract A nanocomposite of graphene oxide and gold nanourchins has been used here to modify the surface of a screen‐printed carbon electrode to enhance the sensitivity of the electrochemical DNA detection system. A specific single‐stranded DNA probe was designed based on the target DNA sequence and was thiolated to be self‐assembled on the surface of the gold nanourchins placed on the modified electrode. Doxorubicin was used as an electrochemical label to detect the DNA hybridisation using differential pulse voltammetry (DPV). The assembling process was confirmed using scanning electron microscopy (SEM) imaging, cyclic voltammetry (CV), and the EIS method. The high sensitivity of the proposed system led to a low detection limit of 0.16 fM and a wide linear range from 0.5 to 950.0 fM. The specificity of the DNA hybridisation and the signalling molecule (haematoxylin) caused very high selectivity towards the target DNA than other non‐specific sequences.https://doi.org/10.1049/nbt2.12086biosensorsDNAelectrochemical sensorsnanocomposites |
| spellingShingle | Mostafa Azimzadeh Zahra Aghili Behrooz Jannat Saeid Jafari Saeed Rafizadeh Tafti Navid Nasirizadeh Nanocomposite of electrochemically reduced graphene oxide and gold nanourchins for electrochemical DNA detection IET Nanobiotechnology biosensors DNA electrochemical sensors nanocomposites |
| title | Nanocomposite of electrochemically reduced graphene oxide and gold nanourchins for electrochemical DNA detection |
| title_full | Nanocomposite of electrochemically reduced graphene oxide and gold nanourchins for electrochemical DNA detection |
| title_fullStr | Nanocomposite of electrochemically reduced graphene oxide and gold nanourchins for electrochemical DNA detection |
| title_full_unstemmed | Nanocomposite of electrochemically reduced graphene oxide and gold nanourchins for electrochemical DNA detection |
| title_short | Nanocomposite of electrochemically reduced graphene oxide and gold nanourchins for electrochemical DNA detection |
| title_sort | nanocomposite of electrochemically reduced graphene oxide and gold nanourchins for electrochemical dna detection |
| topic | biosensors DNA electrochemical sensors nanocomposites |
| url | https://doi.org/10.1049/nbt2.12086 |
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