Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 Pair
Abstract Ovarian dysfunction is a major factor leading to female infertility. Understanding how to improve or reshape ovarian function has become an important entry point for preventing and treating female infertility caused by ovarian dysfunction. Here, plant‐derived compounds are screened for in v...
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Wiley
2025-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202410098 |
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| author | Chunlan Mu Xiaoyong Li Jiamei Yang Geng G. Tian Hepeng Bai Wenhui Lin Linhui Wang Ji Wu |
| author_facet | Chunlan Mu Xiaoyong Li Jiamei Yang Geng G. Tian Hepeng Bai Wenhui Lin Linhui Wang Ji Wu |
| author_sort | Chunlan Mu |
| collection | DOAJ |
| description | Abstract Ovarian dysfunction is a major factor leading to female infertility. Understanding how to improve or reshape ovarian function has become an important entry point for preventing and treating female infertility caused by ovarian dysfunction. Here, plant‐derived compounds are screened for in vitro activity upon ovarian organoids derived from feeder‐free female germline stem cells. Tetrahydroxy stilbene glucoside (TSG) is found to promote the development of ovarian organoids. Single nucleus transcriptome sequencing and spatial transcriptome sequencing are used to establish a comprehensive spatiotemporal map to elucidate the role of TSG in ovarian organoid development, encompassing cell types and subtypes, transcription factors, pseudo‐time sequence, and cell communication dynamics. This analysis indicates that TSG promotes ovarian organoid development through the vascular endothelial growth factor A‐Eph receptor B2 ligand‐receptor pair between granulosa cells and oocytes. This study has enhanced the understanding of the mechanisms of ovarian organoid development, establishes a technical platform for screening compounds for treating infertility and related diseases, and lays a foundation for clinically applying plant‐derived compounds. |
| format | Article |
| id | doaj-art-2529cef0a6c143aaa39aa83e9e274c17 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Advanced Science |
| spelling | doaj-art-2529cef0a6c143aaa39aa83e9e274c172025-01-29T09:50:19ZengWileyAdvanced Science2198-38442025-01-01124n/an/a10.1002/advs.202410098Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 PairChunlan Mu0Xiaoyong Li1Jiamei Yang2Geng G. Tian3Hepeng Bai4Wenhui Lin5Linhui Wang6Ji Wu7School of Basic Medical Sciences Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education Ningxia Medical University Yinchuan 750004 ChinaKey Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education) Bio‐X Institutes Shanghai Jiao Tong University Shanghai 200240 ChinaSchool of Basic Medical Sciences Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education Ningxia Medical University Yinchuan 750004 ChinaSchool of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 ChinaKey Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education) Bio‐X Institutes Shanghai Jiao Tong University Shanghai 200240 ChinaSchool of Life Sciences and Biotechnology Joint International Research Laboratory of Metabolic and Developmental Sciences Shanghai Jiao Tong University Shanghai 200240 ChinaDepartment of Urology, Changhai Hospital Second Military Medical University (Naval Medical University) Shanghai 200433 ChinaSchool of Basic Medical Sciences Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education Ningxia Medical University Yinchuan 750004 ChinaAbstract Ovarian dysfunction is a major factor leading to female infertility. Understanding how to improve or reshape ovarian function has become an important entry point for preventing and treating female infertility caused by ovarian dysfunction. Here, plant‐derived compounds are screened for in vitro activity upon ovarian organoids derived from feeder‐free female germline stem cells. Tetrahydroxy stilbene glucoside (TSG) is found to promote the development of ovarian organoids. Single nucleus transcriptome sequencing and spatial transcriptome sequencing are used to establish a comprehensive spatiotemporal map to elucidate the role of TSG in ovarian organoid development, encompassing cell types and subtypes, transcription factors, pseudo‐time sequence, and cell communication dynamics. This analysis indicates that TSG promotes ovarian organoid development through the vascular endothelial growth factor A‐Eph receptor B2 ligand‐receptor pair between granulosa cells and oocytes. This study has enhanced the understanding of the mechanisms of ovarian organoid development, establishes a technical platform for screening compounds for treating infertility and related diseases, and lays a foundation for clinically applying plant‐derived compounds.https://doi.org/10.1002/advs.202410098ligand‐receptor pairsovarian organoidsplant‐derived compoundssingle nucleus transcriptome sequencingspatial transcriptome sequencing |
| spellingShingle | Chunlan Mu Xiaoyong Li Jiamei Yang Geng G. Tian Hepeng Bai Wenhui Lin Linhui Wang Ji Wu Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 Pair Advanced Science ligand‐receptor pairs ovarian organoids plant‐derived compounds single nucleus transcriptome sequencing spatial transcriptome sequencing |
| title | Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 Pair |
| title_full | Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 Pair |
| title_fullStr | Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 Pair |
| title_full_unstemmed | Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 Pair |
| title_short | Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 Pair |
| title_sort | spatial transcriptome and single nucleus transcriptome sequencing reveals tetrahydroxy stilbene glucoside promotes ovarian organoids development through the vegfa ephb2 pair |
| topic | ligand‐receptor pairs ovarian organoids plant‐derived compounds single nucleus transcriptome sequencing spatial transcriptome sequencing |
| url | https://doi.org/10.1002/advs.202410098 |
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