The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model
Introduction. Acute kidney injury (AKI) pathogenesis is complex. Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients. Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis. We aimed to investigate the e...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2013/313528 |
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| author | Ender Hur Alev Garip Asuman Camyar Sibel Ilgun Melih Ozisik Sena Tuna Murat Olukman Zehra Narli Ozdemir Eser Yildirim Sozmen Sait Sen Fehmi Akcicek Soner Duman |
| author_facet | Ender Hur Alev Garip Asuman Camyar Sibel Ilgun Melih Ozisik Sena Tuna Murat Olukman Zehra Narli Ozdemir Eser Yildirim Sozmen Sait Sen Fehmi Akcicek Soner Duman |
| author_sort | Ender Hur |
| collection | DOAJ |
| description | Introduction. Acute kidney injury (AKI) pathogenesis is complex. Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients. Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis. We aimed to investigate the effect of vitamin D in an experimental rat model of gentamicin-induced AKI. Methods. Thirty nonuremic Wistar albino rats were divided into 3 groups: Control group, 1 mL saline intramuscular (im) daily; Genta group, gentamicin 100 mg/kg/day (im); Genta + vitamin D, gentamicin 100 mg/kg/day (im) in addition to 1α, 25 (OH)2D3 0.4 mcg/kg/day subcutaneously for 8 days. Blood pressures and 24-hour urine were measured. Blood urea and creatinine levels and urine tubular injury markers were measured. Renal histology was semiquantitatively assessed. Results. Urea, creatinine and urine neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 were all increased in Genta group indicating AKI model. Systolic blood pressure decreased, but urine volume and glutathione increased in Genta + Vit D group compared to Control group. Histological scores indicating tubular injury increased in Genta and Genta + Vit D groups. Conclusions. Vitamin D does not seem to be effective on histological findings although it has some beneficial effects via RAS system and a promising effect on antioxidant system. |
| format | Article |
| id | doaj-art-24f6f3a0bf544bbf922f63e706f087e3 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-24f6f3a0bf544bbf922f63e706f087e32025-02-03T01:10:09ZengWileyInternational Journal of Endocrinology1687-83371687-83452013-01-01201310.1155/2013/313528313528The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat ModelEnder Hur0Alev Garip1Asuman Camyar2Sibel Ilgun3Melih Ozisik4Sena Tuna5Murat Olukman6Zehra Narli Ozdemir7Eser Yildirim Sozmen8Sait Sen9Fehmi Akcicek10Soner Duman11Department of Nephrology, Bulent Ecevit University Medical School, Esenkoy, 67100 Zonguldak, TurkeyDepartment of Internal Medicine, Ege University Medical School, 35100 Izmir, TurkeyDepartment of Internal Medicine, Ege University Medical School, 35100 Izmir, TurkeyDepartment of Internal Medicine, Ege University Medical School, 35100 Izmir, TurkeyDepartment of Internal Medicine, Ege University Medical School, 35100 Izmir, TurkeyDepartment of Internal Medicine, Ege University Medical School, 35100 Izmir, TurkeyDepartment of Pharmacology, Ege University Medical School, 35100 Izmir, TurkeyDepartment of Internal Medicine, Ege University Medical School, 35100 Izmir, TurkeyDepartment of Biochemistry, Ege University Medical School, 35100 Izmir, TurkeyDepartment of Pathology, Ege University Medical School, 35100 Izmir, TurkeyDepartment of Internal Medicine, Ege University Medical School, 35100 Izmir, TurkeyDepartment of Internal Medicine, Ege University Medical School, 35100 Izmir, TurkeyIntroduction. Acute kidney injury (AKI) pathogenesis is complex. Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients. Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis. We aimed to investigate the effect of vitamin D in an experimental rat model of gentamicin-induced AKI. Methods. Thirty nonuremic Wistar albino rats were divided into 3 groups: Control group, 1 mL saline intramuscular (im) daily; Genta group, gentamicin 100 mg/kg/day (im); Genta + vitamin D, gentamicin 100 mg/kg/day (im) in addition to 1α, 25 (OH)2D3 0.4 mcg/kg/day subcutaneously for 8 days. Blood pressures and 24-hour urine were measured. Blood urea and creatinine levels and urine tubular injury markers were measured. Renal histology was semiquantitatively assessed. Results. Urea, creatinine and urine neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 were all increased in Genta group indicating AKI model. Systolic blood pressure decreased, but urine volume and glutathione increased in Genta + Vit D group compared to Control group. Histological scores indicating tubular injury increased in Genta and Genta + Vit D groups. Conclusions. Vitamin D does not seem to be effective on histological findings although it has some beneficial effects via RAS system and a promising effect on antioxidant system.http://dx.doi.org/10.1155/2013/313528 |
| spellingShingle | Ender Hur Alev Garip Asuman Camyar Sibel Ilgun Melih Ozisik Sena Tuna Murat Olukman Zehra Narli Ozdemir Eser Yildirim Sozmen Sait Sen Fehmi Akcicek Soner Duman The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model International Journal of Endocrinology |
| title | The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model |
| title_full | The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model |
| title_fullStr | The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model |
| title_full_unstemmed | The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model |
| title_short | The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model |
| title_sort | effects of vitamin d on gentamicin induced acute kidney injury in experimental rat model |
| url | http://dx.doi.org/10.1155/2013/313528 |
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