Metabolites Profiling of Manilkara mabokeensis Aubrév Bark and Investigation of Biological Activities
Manilkara mabokeensis Aubrév is a tree that belongs to the Sapotaceae family, native to the tropical forest in Latin America, Asia, Australia, and Africa. The bark of this species is used as traditional medicine to treat diseases. The present study is the first phytochemical investigation on M. mabo...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/4066783 |
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| author | Xavier Worowounga Rami Rahmani Armel-Frederic Namkona Sylvie Cazaux Jean-Laurent Syssa-Magalé Hubert Matondo Jalloul Bouajila |
| author_facet | Xavier Worowounga Rami Rahmani Armel-Frederic Namkona Sylvie Cazaux Jean-Laurent Syssa-Magalé Hubert Matondo Jalloul Bouajila |
| author_sort | Xavier Worowounga |
| collection | DOAJ |
| description | Manilkara mabokeensis Aubrév is a tree that belongs to the Sapotaceae family, native to the tropical forest in Latin America, Asia, Australia, and Africa. The bark of this species is used as traditional medicine to treat diseases. The present study is the first phytochemical investigation on M. mabokeensis Aubrév bark in terms of phytochemical composition and bioactivity. Among the different extracts, ethyl acetate (EtOAc) exhibited the highest values of total phenolic content (TPC), total flavonoid content (TFC), condensed tannin content (CTC), and reducing sugar content, as well as a high antioxidant activity. Interestingly, gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) analysis were enabled to identify 23 compounds in the essential oil of M. mabokeensis Aubrév bark, which have not been previously described in the literature. Phytol and 8,14-cedranoxide were the major identified compounds, with area percentages of 27.9 and 18.8%, respectively. For HPLC analysis, 3,4-dihydroxy-benzoic acid methyl ester showed the highest concentration with 61.8 mg/g of dry residue (dr) among other identified molecules. Manilkara mabokeensis Aubrév bark MeOH extract showed a good anti-15-lipoxygenase (anti-15-LOX) and anti-acetylcholinesterase (anti-AChE) activities of 65.8 and 71.0%, respectively, while it exhibited a moderate antixanthine oxidase (anti-XOD) activity (41.5%) at 50 μg/mL. Furthermore, cyclohexane (CYHA) and ethyl acetate induced the highest cytotoxicity against the human ovarian cancer cell lines, OVCAR (49.5%) and IGROV (48.7%), respectively. Taken together, obtained results argue that M. mabokeensis Aubrév bark is an excellent source of natural compounds and justify its use in folk medicine. |
| format | Article |
| id | doaj-art-24ce25a3d5a3462ba85a2d5d6ae76090 |
| institution | Kabale University |
| issn | 1687-8779 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-24ce25a3d5a3462ba85a2d5d6ae760902025-02-03T05:53:38ZengWileyInternational Journal of Analytical Chemistry1687-87792022-01-01202210.1155/2022/4066783Metabolites Profiling of Manilkara mabokeensis Aubrév Bark and Investigation of Biological ActivitiesXavier Worowounga0Rami Rahmani1Armel-Frederic Namkona2Sylvie Cazaux3Jean-Laurent Syssa-Magalé4Hubert Matondo5Jalloul Bouajila6Laboratoire de Génie ChimiqueResearch Unit of Valorisation of Actives BiomoleculesLaboratoire de Génie ChimiqueLaboratoire de Génie ChimiqueLaboratoire d’Analyse, d’Architecture et de Réactivité des Substances Naturelles (LAARSN)Laboratoire de Génie ChimiqueLaboratoire de Génie ChimiqueManilkara mabokeensis Aubrév is a tree that belongs to the Sapotaceae family, native to the tropical forest in Latin America, Asia, Australia, and Africa. The bark of this species is used as traditional medicine to treat diseases. The present study is the first phytochemical investigation on M. mabokeensis Aubrév bark in terms of phytochemical composition and bioactivity. Among the different extracts, ethyl acetate (EtOAc) exhibited the highest values of total phenolic content (TPC), total flavonoid content (TFC), condensed tannin content (CTC), and reducing sugar content, as well as a high antioxidant activity. Interestingly, gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) analysis were enabled to identify 23 compounds in the essential oil of M. mabokeensis Aubrév bark, which have not been previously described in the literature. Phytol and 8,14-cedranoxide were the major identified compounds, with area percentages of 27.9 and 18.8%, respectively. For HPLC analysis, 3,4-dihydroxy-benzoic acid methyl ester showed the highest concentration with 61.8 mg/g of dry residue (dr) among other identified molecules. Manilkara mabokeensis Aubrév bark MeOH extract showed a good anti-15-lipoxygenase (anti-15-LOX) and anti-acetylcholinesterase (anti-AChE) activities of 65.8 and 71.0%, respectively, while it exhibited a moderate antixanthine oxidase (anti-XOD) activity (41.5%) at 50 μg/mL. Furthermore, cyclohexane (CYHA) and ethyl acetate induced the highest cytotoxicity against the human ovarian cancer cell lines, OVCAR (49.5%) and IGROV (48.7%), respectively. Taken together, obtained results argue that M. mabokeensis Aubrév bark is an excellent source of natural compounds and justify its use in folk medicine.http://dx.doi.org/10.1155/2022/4066783 |
| spellingShingle | Xavier Worowounga Rami Rahmani Armel-Frederic Namkona Sylvie Cazaux Jean-Laurent Syssa-Magalé Hubert Matondo Jalloul Bouajila Metabolites Profiling of Manilkara mabokeensis Aubrév Bark and Investigation of Biological Activities International Journal of Analytical Chemistry |
| title | Metabolites Profiling of Manilkara mabokeensis Aubrév Bark and Investigation of Biological Activities |
| title_full | Metabolites Profiling of Manilkara mabokeensis Aubrév Bark and Investigation of Biological Activities |
| title_fullStr | Metabolites Profiling of Manilkara mabokeensis Aubrév Bark and Investigation of Biological Activities |
| title_full_unstemmed | Metabolites Profiling of Manilkara mabokeensis Aubrév Bark and Investigation of Biological Activities |
| title_short | Metabolites Profiling of Manilkara mabokeensis Aubrév Bark and Investigation of Biological Activities |
| title_sort | metabolites profiling of manilkara mabokeensis aubrev bark and investigation of biological activities |
| url | http://dx.doi.org/10.1155/2022/4066783 |
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