Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citri
Abstract Citrus red mites (P. citri) are key pests affecting citrus production worldwide due to pesticide resistance. The resistance mechanisms of ten pesticides are known, but a comprehensive study using transcriptome data is missing. This study employed deeptools, cuffdiff, rmats, bcftools and oth...
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Nature Portfolio
2024-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-74820-8 |
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| author | Xiaoqing Han Mengyuan Peng Yunlong Zhang Pan Wu Xiaoyu Zheng Xinze Zhang Shuchen Guo Yanhong Ding Na Yang Meng Li Yidong Lv Yi Zhang Shuzhen Liu Guohua Yu Bin Liu Liujun Tian Chongbin Sun |
| author_facet | Xiaoqing Han Mengyuan Peng Yunlong Zhang Pan Wu Xiaoyu Zheng Xinze Zhang Shuchen Guo Yanhong Ding Na Yang Meng Li Yidong Lv Yi Zhang Shuzhen Liu Guohua Yu Bin Liu Liujun Tian Chongbin Sun |
| author_sort | Xiaoqing Han |
| collection | DOAJ |
| description | Abstract Citrus red mites (P. citri) are key pests affecting citrus production worldwide due to pesticide resistance. The resistance mechanisms of ten pesticides are known, but a comprehensive study using transcriptome data is missing. This study employed deeptools, cuffdiff, rmats, bcftools and other software to examine gene expression variation, alternative splicing (AS), and mutations in mite resistance. The research highlighted that pesticides can regulate gene transcription, and red mites with resistance increase cytochrome P450, glutathione S-transferases, carboxylesterase, and acetylcholinesterase expression. Pyridaben also induces new AS events. Fluazinam-induced mites show mRNA splicing peaking earlier than transcription, both peaking at one day and returning to baseline after two days. AS profiles are similar in different mite populations with overlapping pesticide resistances. Lastly, specific mitochondrial SNPs in mites might mediate resistance against select pesticides. |
| format | Article |
| id | doaj-art-24b60c5bae8d40ad8dcf1d7d1603f489 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-24b60c5bae8d40ad8dcf1d7d1603f4892025-08-20T02:18:31ZengNature PortfolioScientific Reports2045-23222024-10-0114111310.1038/s41598-024-74820-8Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citriXiaoqing Han0Mengyuan Peng1Yunlong Zhang2Pan Wu3Xiaoyu Zheng4Xinze Zhang5Shuchen Guo6Yanhong Ding7Na Yang8Meng Li9Yidong Lv10Yi Zhang11Shuzhen Liu12Guohua Yu13Bin Liu14Liujun Tian15Chongbin Sun16Central Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai UniversityThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityCentral Laboratory of the First Affiliated Hospital, WeiFang People’s Hospital, Shandong Second Medical UniversityAbstract Citrus red mites (P. citri) are key pests affecting citrus production worldwide due to pesticide resistance. The resistance mechanisms of ten pesticides are known, but a comprehensive study using transcriptome data is missing. This study employed deeptools, cuffdiff, rmats, bcftools and other software to examine gene expression variation, alternative splicing (AS), and mutations in mite resistance. The research highlighted that pesticides can regulate gene transcription, and red mites with resistance increase cytochrome P450, glutathione S-transferases, carboxylesterase, and acetylcholinesterase expression. Pyridaben also induces new AS events. Fluazinam-induced mites show mRNA splicing peaking earlier than transcription, both peaking at one day and returning to baseline after two days. AS profiles are similar in different mite populations with overlapping pesticide resistances. Lastly, specific mitochondrial SNPs in mites might mediate resistance against select pesticides.https://doi.org/10.1038/s41598-024-74820-8 |
| spellingShingle | Xiaoqing Han Mengyuan Peng Yunlong Zhang Pan Wu Xiaoyu Zheng Xinze Zhang Shuchen Guo Yanhong Ding Na Yang Meng Li Yidong Lv Yi Zhang Shuzhen Liu Guohua Yu Bin Liu Liujun Tian Chongbin Sun Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citri Scientific Reports |
| title | Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citri |
| title_full | Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citri |
| title_fullStr | Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citri |
| title_full_unstemmed | Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citri |
| title_short | Transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in Panonychus citri |
| title_sort | transcriptome reanalysis and gene expression of 13 detoxification genes for avermectin and pyridaben resistance in panonychus citri |
| url | https://doi.org/10.1038/s41598-024-74820-8 |
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