Ecofriendly Synthesis of Silver Nanoparticles from Garden Rhubarb (Rheum rhabarbarum)
Bioreduction of silver ions following one pot process is described to achieve Rheum rhabarbarum (RR) based silver nanoparticles (SNPs) which is termed as “RR-SNPs.” The Ultraviolet–visible spectroscopy (UV–vis) confirms the characteristic surface plasmon resonance band for RR-SNPs in the range of 42...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2016/4964752 |
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| author | Palem Ramasubba Reddy Shimoga D. Ganesh Nabanita Saha Oyunchimeg Zandraa Petr Sáha |
| author_facet | Palem Ramasubba Reddy Shimoga D. Ganesh Nabanita Saha Oyunchimeg Zandraa Petr Sáha |
| author_sort | Palem Ramasubba Reddy |
| collection | DOAJ |
| description | Bioreduction of silver ions following one pot process is described to achieve Rheum rhabarbarum (RR) based silver nanoparticles (SNPs) which is termed as “RR-SNPs.” The Ultraviolet–visible spectroscopy (UV–vis) confirms the characteristic surface plasmon resonance band for RR-SNPs in the range of 420–460 nm. The crystalline nature of SNPs was confirmed by X-ray diffraction (XRD) peaks at 38.2°, 45.6°, 64.2°, and 76.8°. Transmission electron microscopy (TEM) and scanning electronic microscopy (SEM) confirm the shape of synthesized SNPs. They are roughly spherical but uniformly distributed, and size varies from 60 to 80 nm. These biogenic SNPs show persistent zeta potential value of 34.8 mV even after 120 days and exhibit potent antibacterial activity in presence of Escherichia coli (CCM 4517) and Staphylococcus aureus (CCM 4516). In addition, cytotoxicity of RR-SNPs against in vitro human epithelial carcinoma (HeLa) cell line showed a dose-response activity. The lethal concentration (LC50) value was found to be 28.5 μg/mL for RR-SNPs in the presence of HeLa cells. These findings help us to evaluate their appropriate applications in the field of nanotechnology and nanomedicine. |
| format | Article |
| id | doaj-art-249e437f7b634207928de0d0c2516343 |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-249e437f7b634207928de0d0c25163432025-02-03T06:45:28ZengWileyJournal of Nanotechnology1687-95031687-95112016-01-01201610.1155/2016/49647524964752Ecofriendly Synthesis of Silver Nanoparticles from Garden Rhubarb (Rheum rhabarbarum)Palem Ramasubba Reddy0Shimoga D. Ganesh1Nabanita Saha2Oyunchimeg Zandraa3Petr Sáha4Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tř. T. Bati 5678, 760 01 Zlin, Czech RepublicCentre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tř. T. Bati 5678, 760 01 Zlin, Czech RepublicCentre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tř. T. Bati 5678, 760 01 Zlin, Czech RepublicCentre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tř. T. Bati 5678, 760 01 Zlin, Czech RepublicCentre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tř. T. Bati 5678, 760 01 Zlin, Czech RepublicBioreduction of silver ions following one pot process is described to achieve Rheum rhabarbarum (RR) based silver nanoparticles (SNPs) which is termed as “RR-SNPs.” The Ultraviolet–visible spectroscopy (UV–vis) confirms the characteristic surface plasmon resonance band for RR-SNPs in the range of 420–460 nm. The crystalline nature of SNPs was confirmed by X-ray diffraction (XRD) peaks at 38.2°, 45.6°, 64.2°, and 76.8°. Transmission electron microscopy (TEM) and scanning electronic microscopy (SEM) confirm the shape of synthesized SNPs. They are roughly spherical but uniformly distributed, and size varies from 60 to 80 nm. These biogenic SNPs show persistent zeta potential value of 34.8 mV even after 120 days and exhibit potent antibacterial activity in presence of Escherichia coli (CCM 4517) and Staphylococcus aureus (CCM 4516). In addition, cytotoxicity of RR-SNPs against in vitro human epithelial carcinoma (HeLa) cell line showed a dose-response activity. The lethal concentration (LC50) value was found to be 28.5 μg/mL for RR-SNPs in the presence of HeLa cells. These findings help us to evaluate their appropriate applications in the field of nanotechnology and nanomedicine.http://dx.doi.org/10.1155/2016/4964752 |
| spellingShingle | Palem Ramasubba Reddy Shimoga D. Ganesh Nabanita Saha Oyunchimeg Zandraa Petr Sáha Ecofriendly Synthesis of Silver Nanoparticles from Garden Rhubarb (Rheum rhabarbarum) Journal of Nanotechnology |
| title | Ecofriendly Synthesis of Silver Nanoparticles from Garden Rhubarb (Rheum rhabarbarum) |
| title_full | Ecofriendly Synthesis of Silver Nanoparticles from Garden Rhubarb (Rheum rhabarbarum) |
| title_fullStr | Ecofriendly Synthesis of Silver Nanoparticles from Garden Rhubarb (Rheum rhabarbarum) |
| title_full_unstemmed | Ecofriendly Synthesis of Silver Nanoparticles from Garden Rhubarb (Rheum rhabarbarum) |
| title_short | Ecofriendly Synthesis of Silver Nanoparticles from Garden Rhubarb (Rheum rhabarbarum) |
| title_sort | ecofriendly synthesis of silver nanoparticles from garden rhubarb rheum rhabarbarum |
| url | http://dx.doi.org/10.1155/2016/4964752 |
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