The Production of an Economical Culture Medium from Apple Pomace for the Propagation of Non-Conventional Cidermaking Yeast Starters
The aim of the present study was to evaluate the use of different chemical treatments of apple pomace in order to produce an economical culture medium for the propagation of two non-conventional yeast strains. An experimental design approach was used for the optimization of the hydrolysis conditions...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/1/33 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588468417462272 |
|---|---|
| author | Josefina M. Fontanini Andrea C. Origone Marcela P. Sangorrín Carmen R. Maturano Christian A. Lopes M. Eugenia Rodríguez |
| author_facet | Josefina M. Fontanini Andrea C. Origone Marcela P. Sangorrín Carmen R. Maturano Christian A. Lopes M. Eugenia Rodríguez |
| author_sort | Josefina M. Fontanini |
| collection | DOAJ |
| description | The aim of the present study was to evaluate the use of different chemical treatments of apple pomace in order to produce an economical culture medium for the propagation of two non-conventional yeast strains. An experimental design approach was used for the optimization of the hydrolysis conditions of apple pomace. Both acid and alkaline treatment conditions were tested. The optimal hydrolysis conditions to disrupt the pomace lignocellulosic structure were 1% <i>w</i>/<i>v</i> of H<sub>3</sub>PO<sub>4</sub>, 121 °C, and 40 min for acid treatment, while 6% <i>w</i>/<i>v</i> of NH<sub>4</sub>OH, 20 °C, and 2 h were optimal for the alkaline condition. <i>Saccharomyces uvarum</i> NPCC 1420 and <i>Saccharomyces eubayanus</i> NPCC 1292 yeasts were able to grow in the liquid fraction obtained from both acid and alkaline treatments. However, the medium with the acid treatment was found to be more suitable for yeast growth, showing, for both strains, higher µmax and biomass production and lower td and λ than that observed for the medium with the alkaline treatment. According to the growth parameter analysis for both strains, the acid treatment was selected for further studies. By taking advantage of this agroindustrial by-product, a circular economy approach is promoted, reducing environmental impact and fostering sustainable development. |
| format | Article |
| id | doaj-art-2462f03e924d4f03998cbcdb1f5030de |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-2462f03e924d4f03998cbcdb1f5030de2025-01-24T13:32:07ZengMDPI AGFermentation2311-56372025-01-011113310.3390/fermentation11010033The Production of an Economical Culture Medium from Apple Pomace for the Propagation of Non-Conventional Cidermaking Yeast StartersJosefina M. Fontanini0Andrea C. Origone1Marcela P. Sangorrín2Carmen R. Maturano3Christian A. Lopes4M. Eugenia Rodríguez5Institute for Research and Development in Process Engineering, Biotechnology and Alternative Energies, National University of Comahue, Neuquén 8300, ArgentinaInstitute for Research and Development in Process Engineering, Biotechnology and Alternative Energies, National University of Comahue, Neuquén 8300, ArgentinaInstitute for Research and Development in Process Engineering, Biotechnology and Alternative Energies, National University of Comahue, Neuquén 8300, ArgentinaInstitute for Research and Development in Process Engineering, Biotechnology and Alternative Energies, National University of Comahue, Neuquén 8300, ArgentinaInstitute for Research and Development in Process Engineering, Biotechnology and Alternative Energies, National University of Comahue, Neuquén 8300, ArgentinaInstitute for Research and Development in Process Engineering, Biotechnology and Alternative Energies, National University of Comahue, Neuquén 8300, ArgentinaThe aim of the present study was to evaluate the use of different chemical treatments of apple pomace in order to produce an economical culture medium for the propagation of two non-conventional yeast strains. An experimental design approach was used for the optimization of the hydrolysis conditions of apple pomace. Both acid and alkaline treatment conditions were tested. The optimal hydrolysis conditions to disrupt the pomace lignocellulosic structure were 1% <i>w</i>/<i>v</i> of H<sub>3</sub>PO<sub>4</sub>, 121 °C, and 40 min for acid treatment, while 6% <i>w</i>/<i>v</i> of NH<sub>4</sub>OH, 20 °C, and 2 h were optimal for the alkaline condition. <i>Saccharomyces uvarum</i> NPCC 1420 and <i>Saccharomyces eubayanus</i> NPCC 1292 yeasts were able to grow in the liquid fraction obtained from both acid and alkaline treatments. However, the medium with the acid treatment was found to be more suitable for yeast growth, showing, for both strains, higher µmax and biomass production and lower td and λ than that observed for the medium with the alkaline treatment. According to the growth parameter analysis for both strains, the acid treatment was selected for further studies. By taking advantage of this agroindustrial by-product, a circular economy approach is promoted, reducing environmental impact and fostering sustainable development.https://www.mdpi.com/2311-5637/11/1/33lignocellulosic wastescideracid treatmentalkaline treatmentculture mediumbiomass propagation |
| spellingShingle | Josefina M. Fontanini Andrea C. Origone Marcela P. Sangorrín Carmen R. Maturano Christian A. Lopes M. Eugenia Rodríguez The Production of an Economical Culture Medium from Apple Pomace for the Propagation of Non-Conventional Cidermaking Yeast Starters Fermentation lignocellulosic wastes cider acid treatment alkaline treatment culture medium biomass propagation |
| title | The Production of an Economical Culture Medium from Apple Pomace for the Propagation of Non-Conventional Cidermaking Yeast Starters |
| title_full | The Production of an Economical Culture Medium from Apple Pomace for the Propagation of Non-Conventional Cidermaking Yeast Starters |
| title_fullStr | The Production of an Economical Culture Medium from Apple Pomace for the Propagation of Non-Conventional Cidermaking Yeast Starters |
| title_full_unstemmed | The Production of an Economical Culture Medium from Apple Pomace for the Propagation of Non-Conventional Cidermaking Yeast Starters |
| title_short | The Production of an Economical Culture Medium from Apple Pomace for the Propagation of Non-Conventional Cidermaking Yeast Starters |
| title_sort | production of an economical culture medium from apple pomace for the propagation of non conventional cidermaking yeast starters |
| topic | lignocellulosic wastes cider acid treatment alkaline treatment culture medium biomass propagation |
| url | https://www.mdpi.com/2311-5637/11/1/33 |
| work_keys_str_mv | AT josefinamfontanini theproductionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT andreacorigone theproductionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT marcelapsangorrin theproductionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT carmenrmaturano theproductionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT christianalopes theproductionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT meugeniarodriguez theproductionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT josefinamfontanini productionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT andreacorigone productionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT marcelapsangorrin productionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT carmenrmaturano productionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT christianalopes productionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters AT meugeniarodriguez productionofaneconomicalculturemediumfromapplepomaceforthepropagationofnonconventionalcidermakingyeaststarters |