Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic induction
The anticancer activity of stevenleaf (SV) on the basis of cell viability, cell cycle, and apoptosis induction in HepG2 cancer cells were evaluated. SV controlled the growth of HepG2 cells with IC50 of 139.82 μmol/L for 24 h, IC50 of 119.12 μmol/L for 48 h and cell cycle arrested at G0/G1 phase, ind...
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| Format: | Article |
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Tsinghua University Press
2020-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453020301427 |
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| author | Sayed Sajid Hussain Fan Zhang Yuanyuan Zhang Kiran Thakur Mahrukh Naudhani Carlos L. Cespedes-Acuña Zhaojun Wei |
| author_facet | Sayed Sajid Hussain Fan Zhang Yuanyuan Zhang Kiran Thakur Mahrukh Naudhani Carlos L. Cespedes-Acuña Zhaojun Wei |
| author_sort | Sayed Sajid Hussain |
| collection | DOAJ |
| description | The anticancer activity of stevenleaf (SV) on the basis of cell viability, cell cycle, and apoptosis induction in HepG2 cancer cells were evaluated. SV controlled the growth of HepG2 cells with IC50 of 139.82 μmol/L for 24 h, IC50 of 119.12 μmol/L for 48 h and cell cycle arrested at G0/G1 phase, induced cell apoptosis and enhanced intracellular ROS generation. For cell cycle arrest, the mRNA expression levels of p21, p27 and p53 were up-regulated, while the expression levels of Cyclin A, Cyclin D1, Cyclin E and CDK1/2 were down-regulated. SV efficiently up-regulated TNF R1, TRADD1 and FADD and down-regulated Caspase8 for cell death receptors; similarly, up-regulated Bax, Bak, Cyt c, Apaf1, Caspase3 and Caspase9, and down-regulated Bcl2, Bcl xl and Bad for mitochondrial signal pathway. SV induced the mTOR-mediated cell apoptosis in HepG2 cells via activation of Akt and AMPK. The mechanistic explanation for the anticancer activity of SV as functional food can be derived from above results. |
| format | Article |
| id | doaj-art-243cfb44b7554ab3aabbd796d403b2a6 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2020-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-243cfb44b7554ab3aabbd796d403b2a62025-02-03T06:47:55ZengTsinghua University PressFood Science and Human Wellness2213-45302020-09-0193295303Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic inductionSayed Sajid Hussain0Fan Zhang1Yuanyuan Zhang2Kiran Thakur3Mahrukh Naudhani4Carlos L. Cespedes-Acuña5Zhaojun Wei6School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, ChinaSchool of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, ChinaSchool of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, ChinaSchool of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, ChinaSchool of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, ChinaDepartment of Basic Sciences, Research Group in Chemistry and Biotechnology of Bioactive Natural Products, Faculty of Sciences, University of Bio-Bío, Andrés Bello Avenue, Chillan, ChileSchool of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, China; College of Biological Science and Technology, Fuzhou University, Fuzhou, 350108, China; Biological Science and Engineering College, North Minzu University, Yinchuan, 750021, China; Corresponding author at: School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People’s Republic of China.The anticancer activity of stevenleaf (SV) on the basis of cell viability, cell cycle, and apoptosis induction in HepG2 cancer cells were evaluated. SV controlled the growth of HepG2 cells with IC50 of 139.82 μmol/L for 24 h, IC50 of 119.12 μmol/L for 48 h and cell cycle arrested at G0/G1 phase, induced cell apoptosis and enhanced intracellular ROS generation. For cell cycle arrest, the mRNA expression levels of p21, p27 and p53 were up-regulated, while the expression levels of Cyclin A, Cyclin D1, Cyclin E and CDK1/2 were down-regulated. SV efficiently up-regulated TNF R1, TRADD1 and FADD and down-regulated Caspase8 for cell death receptors; similarly, up-regulated Bax, Bak, Cyt c, Apaf1, Caspase3 and Caspase9, and down-regulated Bcl2, Bcl xl and Bad for mitochondrial signal pathway. SV induced the mTOR-mediated cell apoptosis in HepG2 cells via activation of Akt and AMPK. The mechanistic explanation for the anticancer activity of SV as functional food can be derived from above results.http://www.sciencedirect.com/science/article/pii/S2213453020301427Gynostemma PentaphyllumStevenleafHepG2 cellCell cycleApoptosis |
| spellingShingle | Sayed Sajid Hussain Fan Zhang Yuanyuan Zhang Kiran Thakur Mahrukh Naudhani Carlos L. Cespedes-Acuña Zhaojun Wei Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic induction Food Science and Human Wellness Gynostemma Pentaphyllum Stevenleaf HepG2 cell Cell cycle Apoptosis |
| title | Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic induction |
| title_full | Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic induction |
| title_fullStr | Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic induction |
| title_full_unstemmed | Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic induction |
| title_short | Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic induction |
| title_sort | stevenleaf from gynostemma pentaphyllum inhibits human hepatoma cell hepg2 through cell cycle arrest and apoptotic induction |
| topic | Gynostemma Pentaphyllum Stevenleaf HepG2 cell Cell cycle Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2213453020301427 |
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