Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological features
Abstract Background We investigated the similarities and differences between two experimental approaches using tachy‐pacing technology to induce desynchronized heart failure in canines. Methods A total of eight dogs were included in the experiment, four were tachy‐paced in right ventricle apex (RVAP...
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Wiley
2025-01-01
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| Series: | Animal Models and Experimental Medicine |
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| Online Access: | https://doi.org/10.1002/ame2.12385 |
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| author | Han Jin Shengwen Yang Hao Huang Sijing Cheng Pengkang He Sixian Weng Min Gu Hongxia Niu Wei Hua Yiran Hu Hui Li |
| author_facet | Han Jin Shengwen Yang Hao Huang Sijing Cheng Pengkang He Sixian Weng Min Gu Hongxia Niu Wei Hua Yiran Hu Hui Li |
| author_sort | Han Jin |
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| description | Abstract Background We investigated the similarities and differences between two experimental approaches using tachy‐pacing technology to induce desynchronized heart failure in canines. Methods A total of eight dogs were included in the experiment, four were tachy‐paced in right ventricle apex (RVAP) and 4 were paced in right atrium after the ablation of left bundle branch to achieve left bundle branch block (RAP+LBBB). Three weeks of follow‐up were conducted to observe the changes in cardiac function and myocardial staining was performed at the end of the experiment. Results Both experimental approaches successfully established heart failure with reduced ejection fraction models, with similar trends in declining cardiac function. The RAP+LBBB group exhibited a prolonged overall ventricular activation time, delayed left ventricular activation, and lesser impact on the right ventricle. The RVAP approach led to a reduction in overall right ventricular compliance and right ventricular enlargement. The RAP+LBBB group exhibited significant reductions in left heart compliance (LVGLS, %: RAP+LBBB −12.60 ± 0.12 to −5.93 ± 1.25; RVAP −13.28 ± 0.62 to −8.05 ± 0.63, p = 0.023; LASct, %: RAP+LBBB −15.75 ± 6.85 to −1.50 ± 1.00; RVAP −15.75 ± 2.87 to −10.05 ± 6.16, p = 0.035). Histological examination revealed more pronounced fibrosis in the left ventricular wall and left atrium in the RAP+LBBB group while the RVAP group showed more prominent fibrosis in the right ventricular myocardium. Conclusion Both approaches establish HFrEF models with comparable trends. The RVAP group shows impaired right ventricular function, while the RAP+LBBB group exhibits more severe decreased compliance and fibrosis in left ventricle. |
| format | Article |
| id | doaj-art-243af8b41b4a4eb8a80f7d84ca977eee |
| institution | Kabale University |
| issn | 2576-2095 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Animal Models and Experimental Medicine |
| spelling | doaj-art-243af8b41b4a4eb8a80f7d84ca977eee2025-02-06T03:52:56ZengWileyAnimal Models and Experimental Medicine2576-20952025-01-018114215310.1002/ame2.12385Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological featuresHan Jin0Shengwen Yang1Hao Huang2Sijing Cheng3Pengkang He4Sixian Weng5Min Gu6Hongxia Niu7Wei Hua8Yiran Hu9Hui Li10Cardiology Department Peking University First Hospital Beijing ChinaDepartment of Heart Center, Beijing Chaoyang Hospital Capital Medical University Beijing ChinaCardiac Arrhythmia Center, Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaCardiac Arrhythmia Center, Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaCardiology Department Peking University First Hospital Beijing ChinaCardiac Arrhythmia Center, Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaCardiac Arrhythmia Center, Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaCardiac Arrhythmia Center, Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaCardiac Arrhythmia Center, Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaCardiac Arrhythmia Center, Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Ultrasound, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaAbstract Background We investigated the similarities and differences between two experimental approaches using tachy‐pacing technology to induce desynchronized heart failure in canines. Methods A total of eight dogs were included in the experiment, four were tachy‐paced in right ventricle apex (RVAP) and 4 were paced in right atrium after the ablation of left bundle branch to achieve left bundle branch block (RAP+LBBB). Three weeks of follow‐up were conducted to observe the changes in cardiac function and myocardial staining was performed at the end of the experiment. Results Both experimental approaches successfully established heart failure with reduced ejection fraction models, with similar trends in declining cardiac function. The RAP+LBBB group exhibited a prolonged overall ventricular activation time, delayed left ventricular activation, and lesser impact on the right ventricle. The RVAP approach led to a reduction in overall right ventricular compliance and right ventricular enlargement. The RAP+LBBB group exhibited significant reductions in left heart compliance (LVGLS, %: RAP+LBBB −12.60 ± 0.12 to −5.93 ± 1.25; RVAP −13.28 ± 0.62 to −8.05 ± 0.63, p = 0.023; LASct, %: RAP+LBBB −15.75 ± 6.85 to −1.50 ± 1.00; RVAP −15.75 ± 2.87 to −10.05 ± 6.16, p = 0.035). Histological examination revealed more pronounced fibrosis in the left ventricular wall and left atrium in the RAP+LBBB group while the RVAP group showed more prominent fibrosis in the right ventricular myocardium. Conclusion Both approaches establish HFrEF models with comparable trends. The RVAP group shows impaired right ventricular function, while the RAP+LBBB group exhibits more severe decreased compliance and fibrosis in left ventricle.https://doi.org/10.1002/ame2.12385dyssynchronyheart failuretachy‐pacing technology |
| spellingShingle | Han Jin Shengwen Yang Hao Huang Sijing Cheng Pengkang He Sixian Weng Min Gu Hongxia Niu Wei Hua Yiran Hu Hui Li Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological features Animal Models and Experimental Medicine dyssynchrony heart failure tachy‐pacing technology |
| title | Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological features |
| title_full | Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological features |
| title_fullStr | Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological features |
| title_full_unstemmed | Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological features |
| title_short | Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological features |
| title_sort | dyssynchronous heart failure models in canines new insights into electrocardiographic echocardiographic and histological features |
| topic | dyssynchrony heart failure tachy‐pacing technology |
| url | https://doi.org/10.1002/ame2.12385 |
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