Interaction of Hyperoside with Human Serum Albumin and Effect of Glucose on the Binding
The interaction of hyperoside (Hyp) with human serum albumin (HSA) and effect of glucose on the binding were studied in simulating physiological condition (pH 7.40). The results suggested that Hyp quenched the endogenous fluorescence of HSA via a static quenching process with the distance of 1.95 nm...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2014/386586 |
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| author | Jie Yang Lingling Qu Wenyue Yang Yun Huang Ning Jiao Wenhong Zhan Ding Zhao Lijian Cui |
| author_facet | Jie Yang Lingling Qu Wenyue Yang Yun Huang Ning Jiao Wenhong Zhan Ding Zhao Lijian Cui |
| author_sort | Jie Yang |
| collection | DOAJ |
| description | The interaction of hyperoside (Hyp) with human serum albumin (HSA) and effect of glucose on the binding were studied in simulating physiological condition (pH 7.40). The results suggested that Hyp quenched the endogenous fluorescence of HSA via a static quenching process with the distance of 1.95 nm between Hyp and HSA. Hydrophobic forces played a major role in stabilizing the Hyp-HSA complex. Through synchronous fluorescence monitoring of conformation of HSA, we found that the binding to Hyp can change the microenvironment around tryptophan (Trp) residues. Increasing in glucose concentration over a range from 0 to 9 mM decreased the binding ability of HSA to Hyp, implying that increasing in glucose concentration would increase the concentration of free Hyp. |
| format | Article |
| id | doaj-art-2434b48fc72345eb812c39176b92898c |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-2434b48fc72345eb812c39176b92898c2025-02-03T01:11:59ZengWileyJournal of Spectroscopy2314-49202314-49392014-01-01201410.1155/2014/386586386586Interaction of Hyperoside with Human Serum Albumin and Effect of Glucose on the BindingJie Yang0Lingling Qu1Wenyue Yang2Yun Huang3Ning Jiao4Wenhong Zhan5Ding Zhao6Lijian Cui7The Third Hospital, Hebei Medical University, Shijiazhuang 050051, ChinaPharmaceutical College, Hebei Medical University, Shijiazhuang 050017, ChinaPharmaceutical College, Hebei Medical University, Shijiazhuang 050017, ChinaPharmaceutical College, Hebei Medical University, Shijiazhuang 050017, ChinaThe Third Hospital, Hebei Medical University, Shijiazhuang 050051, ChinaPharmaceutical College, Hebei Medical University, Shijiazhuang 050017, ChinaPharmaceutical College, Hebei Medical University, Shijiazhuang 050017, ChinaPharmaceutical College, Hebei University of Chinese Medicine, Shijiazhuang 050091, ChinaThe interaction of hyperoside (Hyp) with human serum albumin (HSA) and effect of glucose on the binding were studied in simulating physiological condition (pH 7.40). The results suggested that Hyp quenched the endogenous fluorescence of HSA via a static quenching process with the distance of 1.95 nm between Hyp and HSA. Hydrophobic forces played a major role in stabilizing the Hyp-HSA complex. Through synchronous fluorescence monitoring of conformation of HSA, we found that the binding to Hyp can change the microenvironment around tryptophan (Trp) residues. Increasing in glucose concentration over a range from 0 to 9 mM decreased the binding ability of HSA to Hyp, implying that increasing in glucose concentration would increase the concentration of free Hyp.http://dx.doi.org/10.1155/2014/386586 |
| spellingShingle | Jie Yang Lingling Qu Wenyue Yang Yun Huang Ning Jiao Wenhong Zhan Ding Zhao Lijian Cui Interaction of Hyperoside with Human Serum Albumin and Effect of Glucose on the Binding Journal of Spectroscopy |
| title | Interaction of Hyperoside with Human Serum Albumin and Effect of Glucose on the Binding |
| title_full | Interaction of Hyperoside with Human Serum Albumin and Effect of Glucose on the Binding |
| title_fullStr | Interaction of Hyperoside with Human Serum Albumin and Effect of Glucose on the Binding |
| title_full_unstemmed | Interaction of Hyperoside with Human Serum Albumin and Effect of Glucose on the Binding |
| title_short | Interaction of Hyperoside with Human Serum Albumin and Effect of Glucose on the Binding |
| title_sort | interaction of hyperoside with human serum albumin and effect of glucose on the binding |
| url | http://dx.doi.org/10.1155/2014/386586 |
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