The Acetone Extract of Sclerocarya birrea (Anacardiaceae) Possesses Antiproliferative and Apoptotic Potential against Human Breast Cancer Cell Lines (MCF-7)
Interesting antimicrobial data from the stem bark of Sclerocarya birrea, which support its use in traditional medicine for the treatment of many diseases, have been delineated. The current study was aimed to further study some pharmacological and toxicological properties of the plant to scientifical...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/956206 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546043791671296 |
|---|---|
| author | Nicoline Fri Tanih Roland Ndip Ndip |
| author_facet | Nicoline Fri Tanih Roland Ndip Ndip |
| author_sort | Nicoline Fri Tanih |
| collection | DOAJ |
| description | Interesting antimicrobial data from the stem bark of Sclerocarya birrea, which support its use in traditional medicine for the treatment of many diseases, have been delineated. The current study was aimed to further study some pharmacological and toxicological properties of the plant to scientifically justify its use. Anticancer activity of water and acetone extracts of S. birrea was evaluated on three different cell lines, HT-29, HeLa, and MCF-7 using the cell titre blue viability assay in 96-well plates. Apoptosis was evaluated using the acridine orange and propidium iodide staining method, while morphological structure of treated cells was examined using SEM. The acetone extract exhibited remarkable antiproliferative activities on MCF-7 cell lines at dose- and time-dependent manners (24 h and 48 h of incubation). The extract also exerted apoptotic programmed cell death in MCF-7 cells with significant effect on the DNA. Morphological examination also displayed apoptotic characteristics in the treated cells, including clumping, condensation, and culminating to budding of the cells to produce membrane-bound fragmentation, as well as formation of apoptotic bodies. The acetone extract of S. birrea possesses antiproliferative and apoptotic potential against MCF-7-treated cells and could be further exploited as a potential lead in anticancer therapy. |
| format | Article |
| id | doaj-art-242b9e0240b94c1cbcd318f95651176a |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-242b9e0240b94c1cbcd318f95651176a2025-02-03T07:23:55ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/956206956206The Acetone Extract of Sclerocarya birrea (Anacardiaceae) Possesses Antiproliferative and Apoptotic Potential against Human Breast Cancer Cell Lines (MCF-7)Nicoline Fri Tanih0Roland Ndip Ndip1Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, P/Bag X1314, Alice 5700, South AfricaDepartment of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, P/Bag X1314, Alice 5700, South AfricaInteresting antimicrobial data from the stem bark of Sclerocarya birrea, which support its use in traditional medicine for the treatment of many diseases, have been delineated. The current study was aimed to further study some pharmacological and toxicological properties of the plant to scientifically justify its use. Anticancer activity of water and acetone extracts of S. birrea was evaluated on three different cell lines, HT-29, HeLa, and MCF-7 using the cell titre blue viability assay in 96-well plates. Apoptosis was evaluated using the acridine orange and propidium iodide staining method, while morphological structure of treated cells was examined using SEM. The acetone extract exhibited remarkable antiproliferative activities on MCF-7 cell lines at dose- and time-dependent manners (24 h and 48 h of incubation). The extract also exerted apoptotic programmed cell death in MCF-7 cells with significant effect on the DNA. Morphological examination also displayed apoptotic characteristics in the treated cells, including clumping, condensation, and culminating to budding of the cells to produce membrane-bound fragmentation, as well as formation of apoptotic bodies. The acetone extract of S. birrea possesses antiproliferative and apoptotic potential against MCF-7-treated cells and could be further exploited as a potential lead in anticancer therapy.http://dx.doi.org/10.1155/2013/956206 |
| spellingShingle | Nicoline Fri Tanih Roland Ndip Ndip The Acetone Extract of Sclerocarya birrea (Anacardiaceae) Possesses Antiproliferative and Apoptotic Potential against Human Breast Cancer Cell Lines (MCF-7) The Scientific World Journal |
| title | The Acetone Extract of Sclerocarya birrea (Anacardiaceae) Possesses Antiproliferative and Apoptotic Potential against Human Breast Cancer Cell Lines (MCF-7) |
| title_full | The Acetone Extract of Sclerocarya birrea (Anacardiaceae) Possesses Antiproliferative and Apoptotic Potential against Human Breast Cancer Cell Lines (MCF-7) |
| title_fullStr | The Acetone Extract of Sclerocarya birrea (Anacardiaceae) Possesses Antiproliferative and Apoptotic Potential against Human Breast Cancer Cell Lines (MCF-7) |
| title_full_unstemmed | The Acetone Extract of Sclerocarya birrea (Anacardiaceae) Possesses Antiproliferative and Apoptotic Potential against Human Breast Cancer Cell Lines (MCF-7) |
| title_short | The Acetone Extract of Sclerocarya birrea (Anacardiaceae) Possesses Antiproliferative and Apoptotic Potential against Human Breast Cancer Cell Lines (MCF-7) |
| title_sort | acetone extract of sclerocarya birrea anacardiaceae possesses antiproliferative and apoptotic potential against human breast cancer cell lines mcf 7 |
| url | http://dx.doi.org/10.1155/2013/956206 |
| work_keys_str_mv | AT nicolinefritanih theacetoneextractofsclerocaryabirreaanacardiaceaepossessesantiproliferativeandapoptoticpotentialagainsthumanbreastcancercelllinesmcf7 AT rolandndipndip theacetoneextractofsclerocaryabirreaanacardiaceaepossessesantiproliferativeandapoptoticpotentialagainsthumanbreastcancercelllinesmcf7 AT nicolinefritanih acetoneextractofsclerocaryabirreaanacardiaceaepossessesantiproliferativeandapoptoticpotentialagainsthumanbreastcancercelllinesmcf7 AT rolandndipndip acetoneextractofsclerocaryabirreaanacardiaceaepossessesantiproliferativeandapoptoticpotentialagainsthumanbreastcancercelllinesmcf7 |