Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds
In the present study ethanol and water extracts of 15 seaweeds, Dictyota dichotoma var. velutricata, Dictyota indica, Iyengaria stellata, Padina pavonia, Sargassum swartzii, Sargassum variegatum, Stoechospermum marginatum, Stokeyia indica, Jolyna laminarioides, Caulerpa taxifolia, Halimeda tuna, Ulv...
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2015-01-01
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| Series: | Global Journal of Environmental Science and Management |
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| author | A. Tariq M. Athar J. Ara V. Sultana S. Ehteshamul-Haque M. Ahmad |
| author_facet | A. Tariq M. Athar J. Ara V. Sultana S. Ehteshamul-Haque M. Ahmad |
| author_sort | A. Tariq |
| collection | DOAJ |
| description | In the present study ethanol and water extracts of 15 seaweeds, Dictyota dichotoma var. velutricata, Dictyota indica, Iyengaria stellata, Padina pavonia, Sargassum swartzii, Sargassum variegatum, Stoechospermum marginatum, Stokeyia indica, Jolyna laminarioides, Caulerpa taxifolia, Halimeda tuna, Ulva fasciata, Ulva lactuca, Solieria robusta, and Melanothamnus afaqhusainii, were evaluated for their antioxidant potential by ABTS or 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), superoxide and total antioxidant capacity (TAC) assays. The activity was concentration dependent and the variation in antioxidant potential was also observed by different assays in both extracts. Ethanol extract of D. dichotoma var. velutricata, D. indica and S. marginatum demonstrated highest activity by TAC assay. The antioxidant potential in organic solvent fractions of seaweeds namely P. pavonia, S. swartzii, S. marginatum and M. afaqhusainii was also determined and chloroform fraction of all the four seaweeds showed highest activity by superoxide assay. Antioxidant activity of extracted fractions of polysaccharides from S. indica, C. taxifolia and D. dichotoma var. velutricata was also evaluated by superoxide method. Polysaccharide fractions of S. indica obtained from HCl (at 700C and room temperature) and water extract demonstrated highest activity respectively. All the polysaccharide fractions of C. taxifolia showed excellent activity except CaClF70°C. Polysaccharide fractions of D. dichotoma var. velutricata also exhibited very good activity. |
| format | Article |
| id | doaj-art-2429c23952474bd1ad4f5df79b046d14 |
| institution | Kabale University |
| issn | 2383-3572 2383-3866 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | GJESM Publisher |
| record_format | Article |
| series | Global Journal of Environmental Science and Management |
| spelling | doaj-art-2429c23952474bd1ad4f5df79b046d142025-02-02T20:17:24ZengGJESM PublisherGlobal Journal of Environmental Science and Management2383-35722383-38662015-01-011147629555Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweedsA. Tariq0M. Athar1J. Ara2V. Sultana3S. Ehteshamul-Haque4M. Ahmad5Department of Food Science and Technology, University of Karachi, Karachi-75270, PakistanCalifornia Department of Food and Agriculture, 3288 Meadowview Road, Sacramento, CA 95832, USADepartment of Food Science and Technology, University of Karachi, Karachi-75270, PakistanDepartment of Biochemistry, University of Karachi, Karachi-75270, PakistanDepartment of Botany, University of Karachi, Karachi-75270, PakistanDepartment of Pharmacognosy, University of Karachi, Karachi-75270, PakistanIn the present study ethanol and water extracts of 15 seaweeds, Dictyota dichotoma var. velutricata, Dictyota indica, Iyengaria stellata, Padina pavonia, Sargassum swartzii, Sargassum variegatum, Stoechospermum marginatum, Stokeyia indica, Jolyna laminarioides, Caulerpa taxifolia, Halimeda tuna, Ulva fasciata, Ulva lactuca, Solieria robusta, and Melanothamnus afaqhusainii, were evaluated for their antioxidant potential by ABTS or 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), superoxide and total antioxidant capacity (TAC) assays. The activity was concentration dependent and the variation in antioxidant potential was also observed by different assays in both extracts. Ethanol extract of D. dichotoma var. velutricata, D. indica and S. marginatum demonstrated highest activity by TAC assay. The antioxidant potential in organic solvent fractions of seaweeds namely P. pavonia, S. swartzii, S. marginatum and M. afaqhusainii was also determined and chloroform fraction of all the four seaweeds showed highest activity by superoxide assay. Antioxidant activity of extracted fractions of polysaccharides from S. indica, C. taxifolia and D. dichotoma var. velutricata was also evaluated by superoxide method. Polysaccharide fractions of S. indica obtained from HCl (at 700C and room temperature) and water extract demonstrated highest activity respectively. All the polysaccharide fractions of C. taxifolia showed excellent activity except CaClF70°C. Polysaccharide fractions of D. dichotoma var. velutricata also exhibited very good activity.http://www.gjesm.net/pdf_9555_7a79bd12a017dfd9a334aa7a404aa3f7.htmlAntioxidant activityKarachi coastPolysaccharide fractionsSeaweeds |
| spellingShingle | A. Tariq M. Athar J. Ara V. Sultana S. Ehteshamul-Haque M. Ahmad Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds Global Journal of Environmental Science and Management Antioxidant activity Karachi coast Polysaccharide fractions Seaweeds |
| title | Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds |
| title_full | Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds |
| title_fullStr | Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds |
| title_full_unstemmed | Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds |
| title_short | Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds |
| title_sort | biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds |
| topic | Antioxidant activity Karachi coast Polysaccharide fractions Seaweeds |
| url | http://www.gjesm.net/pdf_9555_7a79bd12a017dfd9a334aa7a404aa3f7.html |
| work_keys_str_mv | AT atariq biochemicalevaluationofantioxidantactivityandpolysaccharidesfractionsinseaweeds AT mathar biochemicalevaluationofantioxidantactivityandpolysaccharidesfractionsinseaweeds AT jara biochemicalevaluationofantioxidantactivityandpolysaccharidesfractionsinseaweeds AT vsultana biochemicalevaluationofantioxidantactivityandpolysaccharidesfractionsinseaweeds AT sehteshamulhaque biochemicalevaluationofantioxidantactivityandpolysaccharidesfractionsinseaweeds AT mahmad biochemicalevaluationofantioxidantactivityandpolysaccharidesfractionsinseaweeds |